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Molecules 2011, 16(12), 10123-10133; doi:10.3390/molecules161210123
Article

The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells

1,†, 2,†, 2,†, 1,†, 2, 2, 1, 1,*  and 1,*
1 Department of Orthopaedic Surgery, Xi Jing Hospital, Fourth Military Medical University, # 88 Jiefang Road, Xi’an 710032, China 2 Department of Orthopaedic Surgery, Southern Hospital, Southern Medical University, # 1838 North Guangzhou Road, Guangzhou 510515, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 4 November 2011 / Revised: 28 November 2011 / Accepted: 30 November 2011 / Published: 6 December 2011
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Abstract

Icariin had been reported as a potential agent for osteogenesis, but the dose-effect relationship needed further research to realize the clinical application of icariin. We isolated and purified human bone mesenchymal stem cells (hBMSCs) and stimulated them with different concentrations of icariin. The cytotoxicity of icariin was evaluated by the methylthiazolytetrazolium (MTT) assay method. The proliferation and osteogenic differentiation of such hBMSCs were investigated for different concentrations of icariin. We found that icariin had a dose-dependent effect on the proliferation and osteogenic differentiation of hBMSCs in a suitable concentration range from 109 M to 106 M, but at concentrations above 105 M, the cytotoxicity limited its use. The extremely low cost of icariin and its high abundance make it appealing for bone regeneration.
Keywords: icariin; dose-effect; human bone mesenchymal stem cells; osteogenic differentiation; proliferation icariin; dose-effect; human bone mesenchymal stem cells; osteogenic differentiation; proliferation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fan, J.-J.; Cao, L.-G.; Wu, T.; Wang, D.-X.; Jin, D.; Jiang, S.; Zhang, Z.-Y.; Bi, L.; Pei, G.-X. The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells. Molecules 2011, 16, 10123-10133.

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