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Molecules 2010, 15(9), 6423-6435; doi:10.3390/molecules15096423
Article

Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells

1,2
, 2
, 1,2
, 3
, 2,4
, 1,2
 and 1,2,*
1 Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, 222 Mai Chin Road, Keelung 204, Taiwan 2 College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 333, Taiwan 3 School of Medicine, Fu Jen Catholic University, Taipei County 242, Taiwan 4 Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Keelung, 204, Taiwan
* Author to whom correspondence should be addressed.
Received: 23 August 2010 / Revised: 8 September 2010 / Accepted: 9 September 2010 / Published: 14 September 2010
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Abstract

The use of indigo naturalis to treat psoriasis has proved effective in our previous clinical studies. The present study was designed to examine the anti-inflammatory effect of indigo naturalis in primary cultured human umbilical vein endothelial cells (HUVECs). Pretreatment of cells with indigo naturalis extract attenuated TNF-α-induced increase in Jurkat T cell adhesion to HUVECs as well as decreased the protein and messenger (m)RNA expression levels of vascular cell adhesion molecule-1 (VCAM-1) on HUVECs. Indigo naturalis extract also inhibited the protein expression of activator protein-1 (AP-1)/c-Jun, a critical transcription factor for the activation of VCAM-1 gene expression. Since the reduction of lymphocyte adhesion to vascular cells by indigo naturalis extract could subsequently reduce the inflammatory reactions caused by lymphocyte infiltration in the epidermal layer and help to improve psoriasis, this study provides a potential mechanism for the anti-inflammatory therapeutic effect of indigo naturalis extract in psoriasis.
Keywords: psoriasis; indigo naturalis; human umbilical vein endothelial cell; vascular cell adhesion-1; c-Jun psoriasis; indigo naturalis; human umbilical vein endothelial cell; vascular cell adhesion-1; c-Jun
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chang, H.-N.; Pang, J.-H.; Yang, S.-H.; Hung, C.-F.; Chiang, C.-H.; Lin, T.-Y.; Lin, Y.-K. Inhibitory Effect of Indigo Naturalis on Tumor Necrosis Factor-α-Induced Vascular Cell Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells. Molecules 2010, 15, 6423-6435.

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