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Bio-guided Isolation of Antioxidant Compounds from Chrysophyllum perpulchrum, a Plant Used in the Ivory Coast Pharmacopeia
Laboratoire de Pharmacodynamie-Biochimique; UFR Biosciences; Université de Cocody, 22 BP 582 Abidjan 22, Ivory Coast
Laboratoire de Pharmacognosie et de Phytochimie de l’Université de Genève, Quai Ernest-Ansermet 30/CH-1211 Geneva 4, Switzerland
Laboratoire de Chimie Organique Biologique; UFR SSMT; Université de Cocody, 22 BP 582 Abidjan 22, Ivory Coast
Laboratoire de Botanique; UFR Biosciences; Université de Cocody, 22 BP 582 Abidjan 22, Ivory Coast
* Author to whom correspondence should be addressed.
Received: 20 August 2010; Accepted: 25 August 2010 / Published: 13 September 2010
Abstract: Chrysophyllum perpulchrum (Sapotaceae) is used in the traditional Ivory Coast pharmacopeia to cure fevers. The extract of C. perpulchrum used for this study was the powdered form obtained from the maceration of the dried plant bark in 96% methanol, followed by evaporation to dryness. In the present study, the antioxidative and radical-scavenging activities of the methanolic extract were studied with three standard biological tests: DPPH reduction, ferric thiocyanate (FTC) lipidic peroxidation inhibition and thiobarbituric acid reacting substances (TBARS). Gallic acid and quercetin were used as references. The total amount of phenolic compounds in the extract was determined by ultraviolet (UV) spectrometry and calculated as gallic acid equivalents. Catechin and two dimeric procyanidins were found to be the compounds responsible for the activities. They were chemically dereplicated in the extract by LC-MS. For quantitation purposes, they were isolated by successive chromatographic methods and characterized by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectrometry. The quantities of these compounds in C. perpulchrum were 5.4% for catechin (P1), and 5.6 and 9.2% for dimers (compounds 2 (P2) and 3 (P3)), respectively. They displayed antioxidant activity with IC50 values of 2.50 ± 0.15 µg/mL (P1), 2.10 ± 0.2 µg/mL (P2) and 2.10 ± 0.1 µg/mL (P3). The total extract, the active fractions and the pure compounds inhibited the lipid peroxidation by the FTC method and the TBARS method in the range of 60%. These values were comparable to those seen for quercetin.
Keywords: Chrysophyllum perpulchrum; Sapotaceae; catechin; dimeric procyanidins; antioxidant activity; free radical scavenging activity; quercetin
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Philippe, B.A.; Karine, N.; Barthélemy, A.K.; Noél, Z.G.; David, N.J.; Joseph, D.A.; Hosttetmann, K. Bio-guided Isolation of Antioxidant Compounds from Chrysophyllum perpulchrum, a Plant Used in the Ivory Coast Pharmacopeia. Molecules 2010, 15, 6386-6398.
Philippe BA, Karine N, Barthélemy AK, Noél ZG, David NJ, Joseph DA, Hosttetmann K. Bio-guided Isolation of Antioxidant Compounds from Chrysophyllum perpulchrum, a Plant Used in the Ivory Coast Pharmacopeia. Molecules. 2010; 15(9):6386-6398.
Philippe, Bidie Alain; Karine, Ndjoko; Barthélemy, Attioua Koffi; Noél, Zirihi Guédé; David, N’guessan Jean; Joseph, Djaman Allico; Hosttetmann, Kurt. 2010. "Bio-guided Isolation of Antioxidant Compounds from Chrysophyllum perpulchrum, a Plant Used in the Ivory Coast Pharmacopeia." Molecules 15, no. 9: 6386-6398.