Abstract: Extracts from Dipteryx alata bark obtained with different solvents (hexane, dichloromethane, ethyl acetate and methanol) were mixed in vitro with Bothrops jararacussu (Bjssu, 40 μg/mL) and Crotalus durissus terrificus (Cdt, 15 μg/mL) snake venoms, and applied to a mouse phrenic nerve-diaphragm preparation to evaluate the possible neutralization of venom effects. Cdt venom neurotoxic effect was not inhibited by any of the extracts, while the neurotoxic and myotoxic actions of Bjssu venom were decreased by the methanolic extract. This inhibition appears to be augmented by tannins. Dichloromethane bark extract inhibited ~40% of Bjssu venom effects and delayed blockade induced by Cdt. The methodology used to determine which extract was active allows inferring that: (i) phenolic acids and flavonoids contained in the methanolic extract plus tannins were responsible mostly for neutralization of Bjssu effects; (ii) terpenoids from the dichloromethane extract may participate in the anti-Cdt and anti-Bjssu venom effects; (iii) a given extract could not inhibit venoms from different species even if those belong to the same family, so it is improper to generalize a certain plant as antiophidian; (iv) different polarity extracts do not present the same inhibitory capability, thus demonstrating the need for characterizing both venom pharmacology and the phytochemistry of medicinal plant compounds
Keywords: Bothrops jararacussu; Crotalus durissus terrificus; medicinal plant extract;myotoxicity; neurotoxicity
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Nazato, V.S.; Rubem-Mauro, L.; Vieira, N.A.G.; Dos Santos Rocha, D., Junior; Glauzer Silva, M.; Santos Lopes, P.; Dal-Belo, C.A.; Cogo, J.C.; Dos Santos, M.G.; Da Cruz-Höfling, M.A.; Oshima-Franco, Y. In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts. Molecules 2010, 15, 5956-5970.
Nazato VS, Rubem-Mauro L, Vieira NAG, Dos Santos Rocha D, Junior, Glauzer Silva M, Santos Lopes P, Dal-Belo CA, Cogo JC, Dos Santos MG, Da Cruz-Höfling MA, Oshima-Franco Y. In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts. Molecules. 2010; 15(9):5956-5970.
Nazato, Virgínia Sbrugnera; Rubem-Mauro, Leandro; Vieira, Nathalia Aparecida Gatto; Dos Santos Rocha, Dimas, Junior; Glauzer Silva, Magali; Santos Lopes, Patricia; Dal-Belo, Cháriston André; Cogo, Jose Carlos; Dos Santos, Marcio Galdino; Da Cruz-Höfling, Maria Alice; Oshima-Franco, Yoko. 2010. "In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts." Molecules 15, no. 9: 5956-5970.