Abstract: The free-radical-scavenging activities of various solvent extracts of Microcos paniculata were evaluated through in vitro model systems, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) and Co (II) EDTA-induced luminol chemiluminescence by flow injection. In all three of these systems the ethyl acetate (EtOAc) extract showed the highest free-radical-scavenging activity compared with the other three (n-BuOH, water and petroleum ether) extracts. Free-radical-scavenging assay-guided chromatographic separation of the EtOAc extract, using a normal-phase and reverse-phase silica gel column chromatography yielded five compounds: a new triterpene named methyl 3b-O-p-hydroxy-E-cinnamoyloxy-2a,23-dihydroxyolean-12-en-28-oate (1), whose spectral data are presented for the first time, together with four known compounds, epicatechin (2), 3-trans-feruloyl maslinic acid (3), maslinic acid (4) and sucrose (5). All of the compounds were isolated from Microcos paniculata for the first time. The compounds were identified by spectroscopic methods. Among them, compound 2 displayed significant free-radical-scavenging activity which is similar to that of standard antioxidant ascorbic acid (VC) and therefore may be a promising natural antioxidant.
Keywords: Microcos paniculata; free-radical-scavenging activities; DPPH; ABTS; Co (II) EDTA-induced luminol chemiluminescence by flow injection
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Fan, H.; Yang, G.-Z.; Zheng, T.; Mei, Z.-N.; Liu, X.-M.; Chen, Y.; Chen, S. Chemical Constituents with Free-Radical-Scavenging Activities from the Stem of Microcos paniculata. Molecules 2010, 15, 5547-5560.
Fan H, Yang G-Z, Zheng T, Mei Z-N, Liu X-M, Chen Y, Chen S. Chemical Constituents with Free-Radical-Scavenging Activities from the Stem of Microcos paniculata. Molecules. 2010; 15(8):5547-5560.
Fan, Hua; Yang, Guang-Zhong; Zheng, Tong; Mei, Zhi-Nan; Liu, Xiang-Ming; Chen, Yu; Chen, Su. 2010. "Chemical Constituents with Free-Radical-Scavenging Activities from the Stem of Microcos paniculata." Molecules 15, no. 8: 5547-5560.