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Relaxant Effect of the Ethanol Extract of Helichrysum plicatum (Asteraceae) on Isolated Rat Ileum Contractions
Institute for Medicinal Plants Research, Tadeuša Košćuška 1, 11000 Belgrade, Serbia
Department of Physiology, Faculty of Medicine, University of Nis, Bulevar dr Zorana Djindjica 81, 18000 Nis, Serbia
Department of Pharmacology, Faculty of Medicine, University of Nis, Bulevar dr Zorana Djindjica 81, 18000 Nis, Serbia
* Author to whom correspondence should be addressed.
Received: 2 March 2010; in revised form: 22 April 2010 / Accepted: 29 April 2010 / Published: 10 May 2010
Abstract: Helichrysum plicatum (Turkish Helichrysum) has been used in folk medicine for the treatment of gastric and hepatic disorders. The aim of the present study was to examine the relaxant activity of an extract of H. plicatum flowers on isolated rat ileum. Segments of ileum of rats were suspended in an organ bath. Cumulative concentrations of H. plicatum ethanol extract induced a relaxant effect on spontaneous rat ileum contractions. H. plicatum extract caused a mean contractile response of 81.68 ± 6.17% (at a dose of 0.01 mg/mL) and 30.08 ± 9.07% (at a dose of 1 mg/mL). A similar effect was observed with papaverine (0.01–3 μg/mL). H. plicatum extract (0.01–1 mg/mL) relaxed high K+ (80 mM) precontractions, an effect similar to that caused by papaverine (0.01–3 μg/mL). The plant extract (0.03–0.3 mg/mL) also induced a significant depression of the cumulative concentration response curve for acetylcholine (5–1500 nM) (p < 0.01). Atropine (140 nM) abolished the acetylcholine effect. The extract (0.03–0.3 mg/mL) reduced the histamine (1–300 nM) and BaCl2 (3–900 μM) induced contractions (p < 0.01). Our results showed the relaxant effect of the ethanol extract of Helichrysum plicatum flowers on the isolated rat intestine Extract of H. plicatum can inhibit the spontaneous ileum contractions and contractions induced by acetylcholine, histamine, barium and potassium ions.
Keywords: spasmolytic; Helichrysum plicatum; ileum; rats
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Bigovic, D.; Brankovic, S.; Kitic, D.; Radenkovic, M.; Jankovic, T.; Savikin, K.; Zivanovic, S. Relaxant Effect of the Ethanol Extract of Helichrysum plicatum (Asteraceae) on Isolated Rat Ileum Contractions. Molecules 2010, 15, 3391-3401.
Bigovic D, Brankovic S, Kitic D, Radenkovic M, Jankovic T, Savikin K, Zivanovic S. Relaxant Effect of the Ethanol Extract of Helichrysum plicatum (Asteraceae) on Isolated Rat Ileum Contractions. Molecules. 2010; 15(5):3391-3401.
Bigovic, Dubravka; Brankovic, Suzana; Kitic, Dusanka; Radenkovic, Mirjana; Jankovic, Teodora; Savikin, Katarina; Zivanovic, Slavoljub. 2010. "Relaxant Effect of the Ethanol Extract of Helichrysum plicatum (Asteraceae) on Isolated Rat Ileum Contractions." Molecules 15, no. 5: 3391-3401.