Molecules 2010, 15(4), 2814-2824; doi:10.3390/molecules15042814
Article

Neuroprotective Effects of Herbal Ethanol Extracts from Gynostemma pentaphyllum in the 6-Hydroxydopamine-Lesioned Rat Model of Parkinson's Disease

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Received: 20 February 2010; in revised form: 25 March 2010 / Accepted: 1 April 2010 / Published: 16 April 2010
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Abstract: 6-Hydroxydopamine administration for 28 days (8 μg/2 μL) reduced the number of tyrosine hydroxylase (TH)-immunopositive neurons to 40.2% in the substantia nigra compared to the intact contralateral side. Dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and norepinephrine levels were reduced to 19.1%, 52.3%, 47.1% and 67.4% in the striatum of 6-hydroxydopamine-lesioned rats compared to the control group, respectively. However, an oral administration of herbal ethanol extracts from Gynostemma pentaphyllum (GP-EX) (10 mg/kg and 30 mg/kg) starting on day 3 post-lesion for 28 days markedly ameliorated the reduction of TH-immunopositive neurons induced by 6-hydroxydopamine-lesioned rat brain from 40.2% to 67.4% and 75.8% in the substantia nigra. GP-EX administration (10 and 30 mg/kg) also recovered the levels of dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and norepinephrine in post-lesion striatum to 64.1% and 65.0%, 77.9% and 89.7%, 82.6% and 90.2%, and 88.1% and 89.2% of the control group. GP-EX at the given doses did not produce any sign of toxicity such as weight loss, diarrhea and vomiting in rats during the 28 day treatment period and four gypenoside derivatives, gynosaponin TN-1, gynosaponin TN-2, gypenoside XLV and gypenoside LXXIV were identified from GP-EX. These results suggest that GP-EX might be helpful in the prevention of Parkinson’s disease.
Keywords: Gynostemma pentaphyllum;6-hydroxydopamine-lesioned rats; tyrosine hydroxylase; dopamine; Parkinson’s disease
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MDPI and ACS Style

Choi, H.S.; Park, M.S.; Kim, S.H.; Hwang, B.Y.; Lee, C.K.; Lee, M.K. Neuroprotective Effects of Herbal Ethanol Extracts from Gynostemma pentaphyllum in the 6-Hydroxydopamine-Lesioned Rat Model of Parkinson's Disease. Molecules 2010, 15, 2814-2824.

AMA Style

Choi HS, Park MS, Kim SH, Hwang BY, Lee CK, Lee MK. Neuroprotective Effects of Herbal Ethanol Extracts from Gynostemma pentaphyllum in the 6-Hydroxydopamine-Lesioned Rat Model of Parkinson's Disease. Molecules. 2010; 15(4):2814-2824.

Chicago/Turabian Style

Choi, Hyun Sook; Park, Mi Sook; Kim, Seung Hwan; Hwang, Bang Yeon; Lee, Chong Kil; Lee, Myung Koo. 2010. "Neuroprotective Effects of Herbal Ethanol Extracts from Gynostemma pentaphyllum in the 6-Hydroxydopamine-Lesioned Rat Model of Parkinson's Disease." Molecules 15, no. 4: 2814-2824.


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