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Characterization and Quantification of Polyphenols in Amazon Grape (Pourouma cecropiifolia Martius)
Department of Agricultural, Food and Nutritional Science, University of Alberta, 410 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada
* Author to whom correspondence should be addressed.
Received: 26 October 2010; in revised form: 18 November 2010 / Accepted: 23 November 2010 / Published: 26 November 2010
Abstract: The phenolic profile of Amazon grape fruit (Pourouma cecropiifolia Martius) was investigated by high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). For this purpose, suitable extraction and liquid chromatographic methods were developed. Anthocyanins, flavonols and chlorogenic acids were found mainly in the peel. Besides the main anthocyanins, i.e. delphinidin 3-glucoside, cyanidin 3-glucoside and cyanidin 3-(6”-malonyl)glucoside, several minor anthocyanins were identified in the peel. Among these, cyanidin 3,5-diglucoside, delphinidin 3-galactoside, cyanidin 3-rutinoside, cyanidin 3-(3”-malonyl)glucoside, malvidin 3-glucoside, pelargonidin 3-glucoside, peonidin 3-glucoside and petunidin 3-glucoside were characterized on the basis of their fragmentation patterns in MS/MS experiments. The total anthocyanin content in the peel was 420.26 ± 3.07 mg kg-1 fresh weight. The pulp contained mainly 5-O-caffeoylquinic acid (210.39 ± 3.43 mg kg-1 fresh weight). Rutin was the predominant flavonol found in Amazon grape (peel 155.45 ± 2.06 mg kg-1 fresh weight and pulp 2.64 ± 1.21 mg kg-1 fresh weight). Total polyphenols content was higher in the peel than in the pulp.
Keywords: Pourouma cecropiifolia Martius; Amazon grape; anthocyanins; polyphenols; LC-MS
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Lopes-Lutz, D.; Dettmann, J.; Nimalaratne, C.; Schieber, A. Characterization and Quantification of Polyphenols in Amazon Grape (Pourouma cecropiifolia Martius). Molecules 2010, 15, 8543-8552.
Lopes-Lutz D, Dettmann J, Nimalaratne C, Schieber A. Characterization and Quantification of Polyphenols in Amazon Grape (Pourouma cecropiifolia Martius). Molecules. 2010; 15(12):8543-8552.
Lopes-Lutz, Daise; Dettmann, Judith; Nimalaratne, Chamila; Schieber, Andreas. 2010. "Characterization and Quantification of Polyphenols in Amazon Grape (Pourouma cecropiifolia Martius)." Molecules 15, no. 12: 8543-8552.