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Molecules 2010, 15(12), 8543-8552; doi:10.3390/molecules15128543

Characterization and Quantification of Polyphenols in Amazon Grape (Pourouma cecropiifolia Martius)

, ,  and *
Department of Agricultural, Food and Nutritional Science, University of Alberta, 410 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada
* Author to whom correspondence should be addressed.
Received: 26 October 2010 / Revised: 18 November 2010 / Accepted: 23 November 2010 / Published: 26 November 2010
(This article belongs to the Section Natural Products)
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The phenolic profile of Amazon grape fruit (Pourouma cecropiifolia Martius) was investigated by high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). For this purpose, suitable extraction and liquid chromatographic methods were developed. Anthocyanins, flavonols and chlorogenic acids were found mainly in the peel. Besides the main anthocyanins, i.e. delphinidin 3-glucoside, cyanidin 3-glucoside and cyanidin 3-(6”-malonyl)glucoside, several minor anthocyanins were identified in the peel. Among these, cyanidin 3,5-diglucoside, delphinidin 3-galactoside, cyanidin 3-rutinoside, cyanidin 3-(3”-malonyl)glucoside, malvidin 3-glucoside, pelargonidin 3-glucoside, peonidin 3-glucoside and petunidin 3-glucoside were characterized on the basis of their fragmentation patterns in MS/MS experiments. The total anthocyanin content in the peel was 420.26 ± 3.07 mg kg-1 fresh weight. The pulp contained mainly 5-O-caffeoylquinic acid (210.39 ± 3.43 mg kg-1 fresh weight). Rutin was the predominant flavonol found in Amazon grape (peel 155.45 ± 2.06 mg kg-1 fresh weight and pulp 2.64 ± 1.21 mg kg-1 fresh weight). Total polyphenols content was higher in the peel than in the pulp.
Keywords: Pourouma cecropiifolia Martius; Amazon grape; anthocyanins; polyphenols; LC-MS Pourouma cecropiifolia Martius; Amazon grape; anthocyanins; polyphenols; LC-MS
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lopes-Lutz, D.; Dettmann, J.; Nimalaratne, C.; Schieber, A. Characterization and Quantification of Polyphenols in Amazon Grape (Pourouma cecropiifolia Martius). Molecules 2010, 15, 8543-8552.

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