Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin
Abstract
:Abbreviations
CAP | Cicer arietinum L. pectin |
MW | molecular weight |
DPPH | 2,2-diphenyl-1-picrylhydrazyl |
[η] | intrinsic viscosity |
HM | high methoxy |
LM | low methoxy |
FTIR | Fourier transform infrared spectroscopy |
DE | degree of esterification |
1. Introduction
2. Results and Discussion
2.1. Physical-chemical properties
Galacturonic acid | 69.0 ± 0.5 |
Galactose | 10.5 ± 0.7 |
Arabinose | 6.9 ± 0.5 |
Rhamnose | 6.1 ± 0.6 |
Glucose | 1.2 ± 0.3 |
Xylose | 0.9 ± 0.1 |
Mannose | 0.4 ± 0.1 |
2.2. Antioxidant activity
3. Experimental
3.1. Materials
3.2. Methods
3.2.1. Pectin extraction
3.2.2. Sugar composition
3.2.3. Degree of esterification (DE)
3.2.4. Intrinsic viscosity
3.2.5. Viscosimetric molecular weight
3.2.6. DPPH scavenging activity
3.2.7. Statistical analysis
4. Conclusions
Acknowledgements
- Sample Availability: Samples of the compounds are available from the authors.
References
- Proctor, P.H.; Reynolds, E.S. Free radicals and disease in Man. Physiol. Chem. Phys. Med. NMR 1984, 16, 175–195. [Google Scholar]
- Tomida, H.; Yasufuku, T.; Fujii, T.; Kondo, Y.; Kai, T.; Anraku, M. Polysaccharides as potential antioxidative compounds for extended-release matrix tablets. Carbohydr. Res. 2010, 345, 82–86. [Google Scholar] [CrossRef]
- Li, S.P.; Zhang, G.H.; Zeng, Q.; Huang, Z.G.; Wang, Y.T.; Dong, T.T.; Tsim, K.W. Hypoglycemic activity of polysaccharide, with antioxidation, isolated from cultured Cordyceps mycelia. Phytomedicine 2006, 13, 428–433. [Google Scholar] [CrossRef]
- Mateos-Aparicio, I.; Mateos-Peinado, C.; Jiménez-Escrig, A.; Rupérez, P. Multifunctional antioxidant activity of polysaccharide fractions from the soybean byproduct okara. Carbohydr. Polym. 2010, 82, 245–250. [Google Scholar] [CrossRef]
- Yang, S.S.; Cheng, K.T.; Lin, Y.S.; Liu, Y.W.; Hou, W.C. Pectin hydroxamic acid exhibit antioxidant activities in vitro. J. Agric. Food Chem. 2004, 52, 4270–4273. [Google Scholar]
- Willats, W.G.T.; Paul Knox, J.; Mikkelsen, J.D. Pectin: new insights into an old polymer are starting to gel. Trends Food Sci. Technol. 2006, 17, 97–104. [Google Scholar] [CrossRef]
- Voragen, A.G.J.; Pilnik, W.; Thibault, J.F.; Axelos, M.A.V.; Renard, C.M.G.C. Pectins. In Food Polysaccharides; Stephen, A.M., Ed.; Marcel Dekker: New York, NY, USA, 1995; pp. 287–339. [Google Scholar]
- Maheri-Sis, N.; Chamani, M.; Sadeghi, A.A.; Mirza-Aghazadeh, A.; Aghajanzadeh-Golshani, A. Nutritional evaluation of kabuli and desi type chickpeas (Cicer arietinum L.) for nutriments using in vitro gas production technique. Afr. J. Biotechnol. 2008, 7, 2946–2951. [Google Scholar]
- Aisa, H.A.; Zhen, C.; Yili, A.; Bahang, R.; Rakhmanberdyeva, R.K.; Sagdullaev, B.T. Polysaccharides from two Cicer species cultivated in China. Chem. Nat. Compd. 2006, 42, 349–350. [Google Scholar] [CrossRef]
- Dopico, B.; Nicolás, G.; Labrador, E. Cell wall localization of the natural substrate of β-galactosidase, the main enzyme reponsible for the autolytic process of Cicer arietinum epicotyl cell walls. Physiol. Plant. 1990, 80, 636–641. [Google Scholar] [CrossRef]
- Sevillano, J.M.; Zarra, I.; Acebes, J.L. Pectin depolymerase activities associated with cell walls from Cicer arietinum L.epicotyl. Plant Cell Physiol. 1997, 38, 1259–1263. [Google Scholar] [CrossRef]
- Kurita, O.; Fujiwara, T.; Yamazaki, E. Characterization of the pectin extracted from citrus peel in the presence of citric acid. Carbohydr. Polym. 2008, 74, 725–730. [Google Scholar] [CrossRef]
- Cardoso, S.M.; Coimbra, M.A.; Lopes da Silva, J.A. Temperature dependence of the formation and melting of pectin-Ca2+ networks: a rheological study. Food Hydrocolloid. 2003, 17, 801–807. [Google Scholar] [CrossRef]
- Chatijigakis, A.K.; Pappas, C.; Proxenia, N.; Kalantzi, O.; Rodis, P.; Polissiou, M. FT-IR spectroscopic determination of the degree of esterification of cell wall pectin from stored peaches and correlation to textural changes. Carbohydr. Polym. 1998, 37, 395–408. [Google Scholar] [CrossRef]
- Gnanasambandan, R.; Proctor, A. Determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy. Food Chem. 2000, 68, 327–332. [Google Scholar] [CrossRef]
- Zoete, V.; Vezin, H.; Bailly, F.; Vergoten, G.; Catteau, J.P.; Bernier, J.L. 4-Mercaptoimidazoles derived from naturally occuring antioxidant ovothiols 2. Computational and experimental approach of the radical scavenging mechanism. Free Radical Res. 2000, 32, 525–533. [Google Scholar] [CrossRef]
- Khasina, E.I.; Kolenchenko, E.A.; Sgrebneva, M.N.; Kovalev, V.V.; Khotimchenko, Y.S. Antioxidant activities of a low etherified pectin from the seagrass Zostera marina. Russ. J. Mar. Biol. 2003, 29, 259–261. [Google Scholar] [CrossRef]
- Chen, H.X.; Zhang, M.; Xie, B.J. Quantification of uronic acids in tea polysaccharides conjugates and its antioxidant properties. J. Agric. Food Chem. 2004, 52, 3333–3336. [Google Scholar] [CrossRef]
- Carvajal-Millán, E.; Rascón-Chu, A.; Márquez-Escalante, J.; Ponce de León, N.; Micard, V.; Gardea, A. Maize bran gum: Extraction, characterization and functional properties. Carbohydr. Polym. 2007, 6, 280–285. [Google Scholar]
- Hou, W.; Hsu, F.L.; Lee, M.H. Yam (Dioscorea batatas Deene) tuber mucilage exhibited antioxidant activities in vitro. Planta Med. 2002, 68, 1072–1076. [Google Scholar] [CrossRef]
© 2010 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Share and Cite
Urias-Orona, V.; Huerta-Oros, J.; Carvajal-Millán, E.; Lizardi-Mendoza, J.; Rascón-Chu, A.; Gardea, A.A. Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin. Molecules 2010, 15, 6948-6955. https://doi.org/10.3390/molecules15106948
Urias-Orona V, Huerta-Oros J, Carvajal-Millán E, Lizardi-Mendoza J, Rascón-Chu A, Gardea AA. Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin. Molecules. 2010; 15(10):6948-6955. https://doi.org/10.3390/molecules15106948
Chicago/Turabian StyleUrias-Orona, Vania, Joselina Huerta-Oros, Elizabeth Carvajal-Millán, Jaime Lizardi-Mendoza, Agustin Rascón-Chu, and Alfonso A. Gardea. 2010. "Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin" Molecules 15, no. 10: 6948-6955. https://doi.org/10.3390/molecules15106948