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Molecules 2010, 15(10), 6948-6955; doi:10.3390/molecules15106948

Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin

1
Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, Hermosillo, Sonora, Mexico
2
Centro de Investigación en Alimentación y Desarrollo, A.C., Faculty of Human Nutrition, Autonomous University of Nuevo León, Nuevo León, Mexico
3
Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera al Varadero Nacional Km 6.6, Col. Las Playitas 85480 Guaymas, Sonora, Mexico
*
Author to whom correspondence should be addressed.
Received: 13 August 2010 / Revised: 6 September 2010 / Accepted: 13 September 2010 / Published: 11 October 2010
(This article belongs to the Section Natural Products)
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Abstract

A pectin (CAP) was extracted from the husk of Cicer arietinum L.. Monosaccharide analysis of CAP revealed the dominance of galacturonic acid and smaller amounts of galactose, arabinose, rhamnose, glucose, xylose and mannose. Viscosimetric analysis showed that the intrinsic viscosity ([η]) and the molecular weight (MW) of CAP were 296 mL/g and 105 kDa, respectively. The degree of esterification (DE = 10%) was determined by FTIR spectroscopy. CAP exhibited a dose-dependent free radical scavenging activity, as shown by its DPPH radical inhibition. At 1.0 mg/mL CAP exhibited a scavenging rate of 29% on DPPH radicals. The evaluation of antioxidant activity suggested that CAP had good potential for DPPH radical scavenging activity and should be explored as a novel potential antioxidant.
Keywords: Cicer arietinum; pectin polysaccharide; FTIR; antioxidant activity Cicer arietinum; pectin polysaccharide; FTIR; antioxidant activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Urias-Orona, V.; Huerta-Oros, J.; Carvajal-Millán, E.; Lizardi-Mendoza, J.; Rascón-Chu, A.; Gardea, A.A. Component Analysis and Free Radicals Scavenging Activity of Cicer arietinum L. Husk Pectin. Molecules 2010, 15, 6948-6955.

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