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Molecules 2009, 14(4), 1438-1447; doi:10.3390/molecules14041438

Chemical Composition and Biological Activities of Essential Oil from Salvia sclarea Plants Regenerated in vitro

1
Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151 Łódź, Poland
2
Institute of General Food Chemistry, Technical University of Łódź, Stefanowskiego 4/10, 90-924 Łódź, Poland
3
Department of Pharmaceutical Biochemistry, Medical University of Łódź, Muszyńskiego 1, 90-151 Łódź, Poland
4
Department of Immunology and Infectious Biology, Institute of Microbiology and Immunology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland
*
Author to whom correspondence should be addressed.
Received: 27 January 2009 / Revised: 23 February 2009 / Accepted: 26 February 2009 / Published: 2 April 2009
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Abstract

The essential oils obtained by hydrodistillation of dried aerial parts of Salvia sclarea L. plants, regenerated in vitro and reproduced from seeds, were analyzed by GC and GC-MS. The oils from in vitro and in vivo plants were compared in respect to their chemical composition as well as antimicrobial and cytotoxic activities. The chemical profiles of both oils were very similar, although the yield of essential oil from in vitro plants was lower (0.1%, v/w) than the oil yield isolated from in vivo S. sclarea plants (0.2%, v/w). Both oils showed antimicrobial and cytotoxic activity. The oil from in vitro regenerated plants of S. sclarea exhibited stronger cytotoxic action against NALM-6 cell lines in comparison with the essential oil from in vivo plants.
Keywords: Salvia sclarea in vitro and in vivo; Essential oil chemical composition; Antimicrobial and cytotoxic activity Salvia sclarea in vitro and in vivo; Essential oil chemical composition; Antimicrobial and cytotoxic activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kuźma, Ł.; Kalemba, D.; Różalski, M.; Różalska, B.; Więckowska-Szakiel, M.; Krajewska, U.; Wysokińska, H. Chemical Composition and Biological Activities of Essential Oil from Salvia sclarea Plants Regenerated in vitro. Molecules 2009, 14, 1438-1447.

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