Next Article in Journal
Bystander or No Bystander for Gene Directed Enzyme Prodrug Therapy
Previous Article in Journal
A New Triterpene Hexaglycoside from the Bark of Kalopanax septemlobus (Thunb.) Koidz.
Article Menu

Article Versions

Export Article

Open AccessArticle
Molecules 2009, 14(11), 4505-4516; doi:10.3390/molecules14114505

Antioxidant Effects of Some Drugs on Immobilization Stress Combined with Cold Restraint Stress

1
Department of Chemistry, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia
2
Institute of Pharmacology, Faculty of Medicine, University of Pristina (Kosovska Mitrovica), Serbia
3
The Health Center, 21000 Novi Sad, Serbia
*
Author to whom correspondence should be addressed.
Received: 7 September 2009 / Revised: 20 October 2009 / Accepted: 9 November 2009 / Published: 10 November 2009
Download PDF [354 KB, uploaded 18 June 2014]

Abstract

The aim of this work was to investigate the effect on antioxidant potential of some commonly used drugs (morphine, tramadol, bromocriptine, haloperidol and azithromycin) on immobilization stress (IS) combined with cold restraint stress (CRS) in the rat. After the drug treatment the animals were kept immobilized in the cold chamber at 4±0.3ºC for 3 hours and then decapitaed and the livers were extracted. The following parameters were determined in the liver homogenate: content of reduced glutathione, activities of catalase, xanthine oxidase, glutathione reductase, glutathione peroxidase, peroxidase, and lipid peroxidation intensity. A battery of biochemical assays was used and the resulting data were statistically analyzed. Combined stress exhibited a prooxidative action (increased catalase activity, lowered content of reduced glutathione). Significantly enhanced catalase activity that was observed in all groups compared to the control indicates that the primary reactive oxygen species (ROS) metabolite is hydrogen peroxide, which decomposes very rapidly (very high catalase activity), thus hindering formation of OH radicals as the most toxic ROS. None of the tested drugs showed a protective effect on combined IS and CRS. The intensity of lipid peroxidation did not change either in the combined stress or under additional influence of the drugs. Probably, under our experimental conditions, the time was not sufficiently long to observe damage of lipid membrane by ROS.
Keywords: immobilization stress; cold restraint stress; morphine; tramadol; bromocriptine; haloperidol; azithromycin; antioxidative status immobilization stress; cold restraint stress; morphine; tramadol; bromocriptine; haloperidol; azithromycin; antioxidative status
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Popovic, M.; Janicijevic-Hudomal, S.; Kaurinovic, B.; Rasic, J.; Trivic, S.; Vojnović, M. Antioxidant Effects of Some Drugs on Immobilization Stress Combined with Cold Restraint Stress. Molecules 2009, 14, 4505-4516.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top