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Molecules 2008, 13(6), 1321-1332; doi:10.3390/molecules13061321
Article

Do Cultivar, Geographical Location and Crop Season Influence Phenolic Profile of Walnut Leaves?

1,2,* , 1
, 1
, 3
 and 1
1 REQUIMTE, Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 164, 4099-030 Porto, Portugal 2 Escola Superior de Tecnologia e de Gestão, Instituto Politécnico de Bragança, Quinta de Sta. Apolónia, Apartado 134, 5301-857 Bragança, Portugal 3 Centre for Biological Engineering, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal
* Author to whom correspondence should be addressed.
Received: 7 May 2008 / Revised: 7 June 2008 / Accepted: 8 June 2008 / Published: 12 June 2008
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Abstract

Walnut leaves from nine different cultivars (Arco, Franquette, Hartley, Lara, Marbot, Mayette, Meylannaise, Parisienne and Rego) were studied for their phenolic compounds. Samples were harvested along three consecutive years, at two different geographical locations, in order to evaluate if significant differences in the phenolics composition can be related with genetic, climatic or geographical factors. Nine compounds (3-caffeoylquinic, 3-p-coumaroylquinic and 4-p-coumaroylquinic acids, quercetin 3- galactoside, quercetin 3-arabinoside, quercetin 3-xyloside, quercetin 3-rhamnoside, a quercetin 3-pentoside derivative and a kaempferol 3-pentoside derivative) were quantified using an HPLC-DAD methodology. The qualitative profiles were identical for all samples, but differences were observed in terms of individual compounds’ contents. Multivariate statistical analysis was carried out, showing that significant differences exist among production years, which can be related to climatic reasons.
Keywords: Walnut leaves; Juglans regia L.; phenolic compounds; flavonoids; HPLCDAD; multivariate analysis Walnut leaves; Juglans regia L.; phenolic compounds; flavonoids; HPLCDAD; multivariate analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Amaral, J.S.; Valentão, P.; Andrade, P.B.; Martins, R.C.; Seabra, R.M. Do Cultivar, Geographical Location and Crop Season Influence Phenolic Profile of Walnut Leaves? Molecules 2008, 13, 1321-1332.

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