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Radical Scavenging and Reducing Power of Salvia mirzayanii Subfractions
Department of Pharmacognosy and Pharmaceutical Sciences Research center, Faculty of Pharmacy, Shiraz University of Medical Sciences, Karafarin St., Shiraz 71345-1583, Iran
Biochemistry Department, Faculty of Medicine, and Infectious Disease Research Center, Hormozgan University of Medical Sciences, Shaheed Naser Blvd., Bandar Abbas 79149-64153, Iran
* Author to whom correspondence should be addressed.
Received: 29 June 2008; in revised form: 19 September 2008 / Accepted: 3 November 2008 / Published: 12 November 2008
Abstract: In this research, the radical scavenging activity and reducing power of the ethyl acetate fraction and subfractions of Salvia mirzayanii (SM) have been investigated. The plant material was initially extracted with ethanol. The fractionation was carried out using liquid-liquid extraction, then the ethyl acetate fraction, which showed the greatest antioxidant activity, was selected. This fraction was submitted to column chromatography on a Sephadex LH 20 column eluted with pure MeOH to obtain subfractions A-G. No significant differences exist between the IC50 of Salvia mirzayanii ethyl acetate subfraction C (IC50 = 37.9 ± 0.85), F (IC50 = 40.05 ± 1.4) and quercetin (38.84 ± 0.86), (P > 0.05), indicating that the radical scavenging capacity of these two subfractions and quercetin (antioxidant standard) were similar. The reducing power of the ethyl acetate fraction was less than that of all subfractions, except for subfraction A. The greatest amount of phenolic compounds was found in subfraction E (55.23 ± 4.2) and the lowest in subfraction F (5.23 ± 0.18). The greatest total flavonoid content was established in subfraction D (1.84 ± 0.01) and the lowest was in subfraction A (0.108 ± 0.007).
Keywords: Salvia mirzayanii; Ethyl acetate subfractions; Antioxidant activity; Phenolic compounds; Flavonoids
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MDPI and ACS Style
Moein, M.R.; Moein, S.; Ahmadizadeh, S. Radical Scavenging and Reducing Power of Salvia mirzayanii Subfractions. Molecules 2008, 13, 2804-2813.
Moein MR, Moein S, Ahmadizadeh S. Radical Scavenging and Reducing Power of Salvia mirzayanii Subfractions. Molecules. 2008; 13(11):2804-2813.
Moein, Mahmood R.; Moein, Soheila; Ahmadizadeh, Saeid. 2008. "Radical Scavenging and Reducing Power of Salvia mirzayanii Subfractions." Molecules 13, no. 11: 2804-2813.