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Entropy 2013, 15(12), 5362-5383; doi:10.3390/e15125362
Article

Ribozyme Activity of RNA Nonenzymatically Polymerized from 3′,5′-Cyclic GMP

1
, 2
, 3
, 4, 4
 and 1,*
1 Fondazione "Istituto Pasteur-Fondazione Cenci-Bolognetti" c/o Dipartimento di Biologia e Biotecnologie "Charles Darwin", "Sapienza" Università di Roma, P.le Aldo Moro, 5, Rome 00185, Italy 2 Istituto di Biologia e Patologia Molecolari, CNR, P.le Aldo Moro, 5, Rome 00185, Italy 3 Dipartimento di Scienze Biochimiche, "Sapienza" Università di Roma, P.le Aldo Moro, 5, Rome 00185, Italy 4 Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, Brno 61265, Czech Republic
* Author to whom correspondence should be addressed.
Received: 13 September 2013 / Revised: 19 November 2013 / Accepted: 22 November 2013 / Published: 3 December 2013
(This article belongs to the Special Issue Entropy and RNA Structure, Folding and Mechanics)
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Abstract

3′,5′-Cyclic GMP spontaneously nonenzymatically polymerizes in a base-catalyzed reaction affording G oligonucleotides. When reacted with fully or partially sequence-complementary RNA (oligo C), the abiotically generated oligo G RNA displays a typical ribozyme activity consisting of terminal ligation accompanied by cleavage of an internal phosphate site of the donor oligonucleotide stem upon attack of the acceptor 3′ terminal OH. This reaction is dubbed Ligation following Intermolecular Cleavage (LIC). In a prebiotic perspective, the ability of oligo G polynucleotides to react with other sequences outlines a simple and possible evolutionary scenario based on the autocatalytic properties of RNA.
Keywords: RNA; RNA polymerization; RNA origin; ribozymes; origin of life RNA; RNA polymerization; RNA origin; ribozymes; origin of life
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pino, S.; Costanzo, G.; Giorgi, A.; Šponer, J.; Šponer, J.E.; Mauro, E.D. Ribozyme Activity of RNA Nonenzymatically Polymerized from 3′,5′-Cyclic GMP. Entropy 2013, 15, 5362-5383.

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