The Applications of Flow Cytometry: Advances, Challenges, and Trends
A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cell Methods".
Deadline for manuscript submissions: 31 August 2024 | Viewed by 4673
Special Issue Editor
Interests: flow cytometry; signal amplification; cell-specific molecular expression levels; bipolar disorder; major depressive disorder; PTSD; multiple sclerosis; acute myocardial infarction
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
Flow cytometry is a well-established and user-friendly single-cell technology that simultaneously measures multiple analyte expression patterns in individual cells. Since its development in the 1960s, it has enabled important analytical and clinical breakthroughs in immunology, cell biology, oncology, bacteriology, infectious disease, rheumatology, and molecular biology.
With its widespread application, flow cytometry has made remarkable progress. Recently, advancements in the field include technological innovations (i.e., spectral cytometry, mass cytometry, and imaging cytometry) and methodological innovations in acquisition and analysis. These advances have rendered flow cytometry an invaluable tool in studies of the immune system and other areas of cell biology.
This Special Issue will provide insights into the applications of using flow cytometry, covering the latest advances, current challenges, and future trends. It aims to broaden our understanding of basic research flow cytometry findings and potentially lead in translating new applications or new protocols into clinical strategies.
We would highly welcome the submission of original article, review, or communication on flow cytometry in research biology or medical science. Interested authors should consult the instructions using the following link: https://www.mdpi.com/journal/cells/instructions.
Dr. David R. Kaplan
Guest Editor
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
Keywords
- flow cytometry
- single cell
- cell function
- T-cell subset
Planned Papers
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Flow-Cytometry Evaluation of Blood Cells-Bound Surface FVIII in Hemophilia A and Different Coagulation States.
Authors: Anjud Al-Mohannadi; Reem Mohammed Yahia; Hani Bibawi; Che-Ann Lachica; Watfa Ahmed; Igor Pavlovski; Giusy Gentilcore; Elkhansa Elbukhari Elgaali; Anila Ejaz; Mohammed Elanbari; Zainab Awada; Mohammed J Al-Kubaisi; Muhammad Elnaggar; Ayman Saleh; Chiara Cugno; Sara Deola
Affiliation: Research Department Sidra Medicine PO Box 26999 Doha Qatar
Abstract: Hemophilia A (HA) is associated with coagulation FVIII insufficiency or inactivity leading to excessive bleeding phenotype. Elevated FVIII on the contrary is associated with thrombophilia, thrombosis, myocardial infarctions, and stroke. Active FVIII (FVIIIa) uses its C2 domain to bind to blood cells’ membranes, consequently carrying out its coagulative function. We developed a re-liable Flow-Cytometry (FC) method for FVIII detection that can be utilized for assessing sur-face-bound FVIII on leukocytes, in different coagulation/clinical states. We analyzed 48 pediatric subjects, encompassing patients with HA, other coagulopathies, venous thrombosis, and normal coagulation. Interestingly, total leukocytes’ surface FVIII showed a declining trend across thrombosis, normal, and hypo-coagulation states. Expectedly, leukocytes of HA patients displayed significantly lower levels of cellular surface FVIII in comparison to patients with thrombosis. However, no significant correlation was observed between circulating levels of FVIII in plasma and the levels of FVIII bound to leukocytes, indicating that the differences in FVIII surface binding are not directly proportional to the availability of FVIII in the circulation and suggesting a specific binding mechanism governing the interaction between FVIII and leukocytes. Intriguingly, when analyzing the distinct blood subpopulations, we observed that surface FVIII levels were signifi-cantly elevated in classical monocytes of thrombosis patients (0.47%) compared to HA patients (0.072%, p=0.0338). Our study highlights the reliability of our FC platform in assessing FVIII abundance on leukocytes’ membranes across coagulation states. Monocytes, particularly in cases of thrombosis, exhibit active binding of FVIII on their surface, suggesting a potential role in the pathophysiology of thrombosis that requires further investigation.