Application of RNA Interference (RNAi) in Agricultural Pest Management

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Crop Protection, Diseases, Pests and Weeds".

Deadline for manuscript submissions: closed (10 March 2024) | Viewed by 1131

Special Issue Editors


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Guest Editor
Chair of Plant Health, Estonian University of Life Sciences, 51014 Tartu, Estonia
Interests: integrated pest management; RNA interference; dsRNA; biopesticide; conservation biology; biological control; insect; crop protection; risk assessment

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Guest Editor
Plant Health Department, Research Triangle Park, GreenLight Biosciences, Medford, NC, USA
Interests: RNA interference; dsRNA; gene silencing; crop protection; biological control; agricultural biotechnology; pest control; biopesticide development

Special Issue Information

Dear Colleagues,

RNA interference (RNAi) technology represents a promising solution for managing outbreaks of pests and pathogens in various agricultural systems. Such approaches (including but not limited to transgenic and spray approaches) may furthermore help minimize reductions in non-target organism populations, with these reductions being a key aim in environmentally sustainable agriculture. However, while considerable advances have been made regarding this crop protection technology, numerous hurdles (biological, technical, social and legal) still stand in the way of its implementation on a wider scale.

The aim and scope of this Special Issue is to facilitate the dissemination of knowledge, data and ideas regarding how RNAi technology can be made more effective against agricultural pests and pathogens (e.g. invertebrates, vertebrates, fungi, weeds and viruses), and how RNAi approaches could push agriculture in a more environmentally sustainable direction. Furthermore, the Special Issue aims to address any other hurdles that prevent or slow progress towards developing such approaches.

We invite authors to submit original research (including short communications), as well as unique reviews and perspectives, that contribute to the evolution and broadening of RNAi’s place in agriculture.

Sub-topics that are especially welcome include (but are not limited to) the following:

  • Enhancing efficacy in otherwise RNAi-insensitive pests and pathogens;
  • Formulation development for enhancing dsRNA stability in vivo, under environmental conditions, and in soils and waters;
  • RNAi within integrated pest management (IPM) strategies;
  • Greenhouse, semi-field or field studies;
  • Socioeconomic aspects related to the implementation of RNAi approaches;
  • Ecotoxicology, risk assessment and biosafety;
  • RNAi’s role in biodiversity conservation in agricultural landscapes;
  • dsRNA seed treatments;
  • Novel molecular targets in agricultural pests and pathogens.

Dr. Jonathan Willow
Dr. Thais B. Rodrigues
Guest Editors

Manuscript Submission Information

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Keywords

  • RNA pesticide formulation
  • dsRNA stability
  • integrated pest management
  • greenhouse
  • semi-field
  • seed treatment
  • biodiversity conservation
  • ecotoxicology
  • risk assessment
  • molecular target

Published Papers (1 paper)

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Research

13 pages, 1041 KiB  
Article
Baseline Susceptibility to a Novel dsRNA-Based Insecticide across US Populations of Colorado Potato Beetle
by Samuel Pallis, Andrei Alyokhin, Brian Manley, Thais B. Rodrigues, Ethann Barnes and Kenneth Narva
Agriculture 2023, 13(12), 2283; https://doi.org/10.3390/agriculture13122283 - 16 Dec 2023
Cited by 1 | Viewed by 935
Abstract
The Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) is an insect defoliator of the potato, Solanum tuberosum L. This species thrives in agricultural environments because of its flexible and complex life history, as well as its ability to evolve insecticide resistance. As [...] Read more.
The Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) is an insect defoliator of the potato, Solanum tuberosum L. This species thrives in agricultural environments because of its flexible and complex life history, as well as its ability to evolve insecticide resistance. As a result, it has become a widely distributed agricultural pest. Ledprona (trade name Calantha) is a recently developed novel double-stranded RNA (dsRNA) insecticide that controls populations of Colorado potato beetle through RNA interference (RNAi). Previous studies have demonstrated the efficacy of ledprona through laboratory, greenhouse, and field studies. Colorado potato beetles from geographically distinct populations are known to vary in their response to insecticides, including experimental compounds based on RNAi. We tested the mortality and foliage consumption of beetles from different areas in the US treated with ledprona and found significant variation in both parameters. The beetles originating from New York were significantly less susceptible to ledprona in leaf disc assays compared to other populations. However, currently there is no evidence of reduced performance of ledprona against that population under field conditions, possibly because intoxicated beetles cannot withstand multiple stressors present in the field. The results of this study confirmed that the ledprona efficacy differs among geographically distinct populations, which may have implications for managing Colorado potato beetles. Full article
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