Differentiation of Heterogeneous Mouse Liver from HCC by Hyperpolarized 13C Magnetic Resonance (Version 2, Revised)
|Reviewer 1 Rodolfo Sacco Cisanello Hospital, Pisa, Italy||Reviewer 2 Melissa Edwards Colorado State University|
Approved with revisions
Approved with revisions
Lev-Cohain, N.; Sapir, G.; Uppala, S.; Nardi-Schreiber, A.; Goldberg, S.N.; Adler-Levy, Y.; Sosna, J.; Gomori, J.M.; Katz-Brull, R. Differentiation of Heterogeneous Mouse Liver from HCC by Hyperpolarized 13C Magnetic Resonance. Sci 2020, 2, 43.
Lev-Cohain N, Sapir G, Uppala S, Nardi-Schreiber A, Goldberg SN, Adler-Levy Y, Sosna J, Gomori JM, Katz-Brull R. Differentiation of Heterogeneous Mouse Liver from HCC by Hyperpolarized 13C Magnetic Resonance. Sci. 2020; 2(2):43.Chicago/Turabian Style
Lev-Cohain, Naama; Sapir, Gal; Uppala, Sivaranjan; Nardi-Schreiber, Atara; Goldberg, Shraga N.; Adler-Levy, Yael; Sosna, Jacob; Gomori, J. M.; Katz-Brull, Rachel. 2020. "Differentiation of Heterogeneous Mouse Liver from HCC by Hyperpolarized 13C Magnetic Resonance." Sci 2, no. 2: 43.
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Cisanello Hospital, Pisa, Italy
The paper is interesting and worth of mentioning. I would like to ask Authors:
-if they have data on larger nodules from the same mouse.
-if Authors think the ratio LDH/ALT could be useful (using 13C MRI) to monitor efficacy of targeted therapy for HCC (see the role of monitoring LDH levels during Sorafenib therapy). Please, The authors should speculate on this matter.
Response to Reviewer 1Sent on 10 Jul 2020 by Naama Lev-Cohain, Gal Sapir, Sivaranjan Uppala, Shraga Nahum Goldberg, Atara Nardi-Schreiber, Yael Adler-Levy, Jacob Sosna, J. Moshe Gomori, Rachel Katz-Brull
Colorado State University
ONLY requested revision: Figure 1 would benefit from the inclusion of an image of the healthy liver tissue as all other figures compare the healthy, heterogeneously inflamed, and HCC tumor livers.
Are there other sources of these liver enzymatic rates (from either healthy or diseased animals) that can be re-calculated to expand your study and exhibit the consistent ratios? In your M&M you mentioned that the info for your healthy mice in Table 1. was obtained from your previous work, but are there other resources for similar information?
Understanding that the primary method is particularly to focus on in vivo and ex vivo models, do you know of any in vitro based methods that further support your results in being able to so clearly differentiate between the inflamed and HCC tissue types?
I understand how and why it was not possible for this study and I would be interested to know if the ratios would be comparable between male and female mice as all male mice were used in this study. I would also like to say that I appreciate your use of available mice and the minimization of sacrificed animals.
Overall, a fascinating study and rather sound.