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Open AccessReviewPost Publication Peer ReviewVersion 1, Original

LC-NMR for Natural Products Analysis: A Journey from an Academic Curiosity to a Robust Analytical Tool (Version 1, Original)

1
Department of Industrial Chemistry, Addis Ababa Science and Technology University, P.O. Box 16417, Addis Ababa, Ethiopia
2
Institute of Applied Synthetic Chemistry, Vienna University of Technology; Getreidemarkt 9/163-AC, A-1060 Vienna, Austria
3
Department of Chemistry, Otago University, P.O. Box 56, Dunedin 9054, New Zealand
*
Author to whom correspondence should be addressed.
Received: 8 April 2019 / Accepted: 30 May 2019 / Published: 14 June 2019
Peer review status: 1st round review Read review reports Apply as reviewer

Reviewer 1 Paweł Kafarski Wroclaw University of Science and Technology, Wroclaw, Poland Reviewer 2 Reviewer 3
Version 1
Original
Approved Reviewer inviting Reviewer inviting
LC–NMR combines the advantage of the outstanding separation power of liquid chromatography (LC) and the superior structural elucidating capability of nuclear magnetic resonance (NMR). NMR has proved that it is a standout detector for LC by providing maximum structural information about plant originated extracts particularly in its isolating ability of isomeric (same molecular formula) and/or isobaric (same molecular weight) compounds as compared to other detectors. The present review provides an overview of the LC–NMR developmental trends and its application in natural products analysis. The different LC–NMR operational modes are described, as well as how technical improvements assist in establishing this powerful technique as an important analytical tool in the analysis of complex plant-derived compounds. On-flow, stop-flow and loop-storage modes, as well as the new offline mode LC–SPE–NMR and capLC-NMR configurations that avoid the ingestion of expensive deuterated solvents throughout the experiment are mentioned. Utilization of cryogenic probe and microprobe technologies which are the other important promising approaches for guaranteeing the sensitivity issues are also described. Concluding remarks and future outlooks are also discussed. View Full-Text
Keywords: separation technique; spectroscopic technique; hyphenated techniques; LC-NMR; natural products separation technique; spectroscopic technique; hyphenated techniques; LC-NMR; natural products
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Gebretsadik, T.; Linert, W.; Thomas, M.; Berhanu, T.; Frew, R. LC-NMR for Natural Products Analysis: A Journey from an Academic Curiosity to a Robust Analytical Tool. Sci 2019, 1, 31.

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1

Reviewer 1

Sent on 29 Aug 2019 by Paweł Kafarski | Approved
Wroclaw University of Science and Technology, Wroclaw, Poland

This is a comprehensive review on an emerging technique in on line identification plantmetabolites.

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