Assessing planting to ensure well-distributed plants is important to achieve high yields. Digital farming has been helpful in these field assessments. However, these techniques are at most times not available for smallholder farmers or low-income regions. Thus, to contribute such producers, we developed two methods to assess intra-row spacing in commercial fields using mobile photos and simple image processing. We assessed a maize field after mechanized planting in 7 and 12 days after planting (DAP) and in two farming systems (conventional and no-till) to acquire images at height of one meter and perpendicular to the ground. In the first method, we used morphological operations based on the HSV scale and the center of mass to extract the region of interest (ROI) corresponding to the maize plant. In the second method, we used local maxima equations (Peaks) to find prominence values corresponding to the maize plant and extract their coordinates. No-till images were deleted due to excessive weeds. Thus, before acquiring the images, it is necessary to remove these elements (e.g., no-till adapted). The methods achieved an overall RMSE of 3.48 cm (<5.63 cm) and R² of 0.90 (>0.71) between the actual and estimated spacing. Precision and recall were higher than 0.88. There was no difference between actual and estimated CV values, except in conventional tillage in 7 DAP using ROI due to leaves overlapping. The method Peaks was more accurate to detect multiple spacing but miss spacing was correctly detected in both methods. However, the larger the plant leaves, the worse the detection. Thus, our proposed methods were satisfactory and are promising for assessing planting in a remote and accessible way. Full article

The Xishuangbanna (XIS) cucumber is an important botanical variety, accumulating high levels of β-carotene (700 μg/100 g) in the endocarp of mature fruit compared with normal green/white flesh types (25–50 μg/100 g, fresh weight). β-carotene is an essential precursor of provitamin A synthesis required for human health, thus XIS cucumber is an appealing germplasm for vitamin A breeding programs. In this review, we highlighted the molecular research progress of XIS cucumber as well as the bottlenecks undermining its utilization in genetic breeding. The XIS cucumber was first reported in 1983; thereafter, the literature on XIS cucumber was sporadic until 2012 and 2013 following QTL mapping of the Ore gene and subsequent cloning of the CsaBCH1 gene, respectively. Whereas QTL mapping studies underlying its flowering time and fruit quality related traits have been reported, fine mapping of their candidate genes remains unknown. Cucumber fruits are mainly consumed at fresh immature stage; however, XIS cucumber accumulates β-carotene at mature fruit state, thus limiting the utilization of β-carotene derived from it. In our opinion, we believe that the production and commercialization of immature orange-fleshed cucumber would gain wider acceptance among consumers. Additionally, we highlighted a comprehensive breeding strategy, precisely for enhanced β-carotene accumulation based on prior studies of XIS cucumber coupled with those from other crops. In MAS, we proposed schematic molecular backcross breeding strategy using lines possessing both ore and fft1.1 loci. This review, therefore, provides insights of XIS cucumber research and opportunities for further genetic breeding. Full article

The conversion of chicken feathers, generated annually worldwide on a large scale as a by-product of the poultry industry into value-added products, has economic and environmental benefits. Protein hydrolysate produced from feathers has attracted significant attention in agriculture as a potential plant growth stimulant. Therefore, a study was established with the aim to produce and characterize chicken feather protein hydrolysate (CFPH) and investigate the effects of this product on the early growth of nursery tea plants. Alkaline hydrolysis was used to produce CFPH with the yield of 165 mg amino acids per gram of feathers. Then, the produced CFPH was applied on nursery tea plants as a soil drench at different doses (0.5, 1, 2, 3, and 4 g L⁻¹) in 2-week intervals until the 10th application. Commercially available fish protein hydrolysate (FPH) was included as a treatment to compare the effects with CFPH. The treatments were arranged in a completely randomized block design with three replications. CFPH and FPH significantly improved the shoot and root growth parameters. Plant height (+98%), leaf number (+61%), shoot dry biomass (+128%), root length (+94%), root surface area (+15%), and root dry biomass (+152%) were significantly increased by the application of CFPH (2 g L⁻¹ dose) compared to control. Although the highest CFPH dosage (4 g L⁻¹) showed a reduction in growth parameters, the values obtained were similar or higher than the untreated control plants. The chlorophyll content (a, b, and total) was enhanced by the CFPH dosage of 1 g L⁻¹, whereas the highest photosynthetic rate was recorded in the CFPH 3 g L⁻¹ treatment. The application of protein hydrolysates (PH) did not positively influence stomatal conductance and intercellular CO₂ concentration. Leaf nitrogen, phosphorous, manganese, and copper were positively affected by the CFPH application. The effect of CFPH on growth parameters was more pronounced than FPH. Our findings reveal that CFPH produced by alkaline hydrolysis could be used as a growth booster in raising vigorous tea nursery plants, which are most suitable for field planting and subsequently higher yields. Full article

The relationships between crop yield and its selected related impact factors has often been explored using ordinary least squares regression (OLSR). However, this model is non-spatial and non-robust. This study first used stepwise regression to identify the main factors affecting winter wheat yield from twelve potential related factors in Yucheng County, China. Next, robust geographically weighted regression (RGWR) was used to explore the spatially non-stationary relationships between wheat yield and its main impact factors. Then, its modeling effect was compared with that of GWR and OLSR. Last, robust geostatistical analysis was conducted for spatial soil management measures in low-yield areas. Results showed that: (i) three main impact factors on wheat yield were identified by stepwise regression, namely soil organic matter, soil total phosphorus, and pH; (ii) the spatially non-stationary effects of the main impact factors on wheat yield were revealed by RGWR but were ignored by OLSR; (iii) RGWR obtained the best modeling effect (RI = 52.31%); (iv) robust geostatistics obtains a better spatial prediction effect and the low-yield areas are mainly located in the northeast and the middle east of the study area. Therefore, the integrated yield-based methodology effectively improves soil nutrient management at a regional scale. Full article

Agriculture consumes an important ratio of the water reserve in irrigated areas. The improvement of irrigation is becoming essential to reduce this high water consumption by adapting supplies to the crop needs and avoiding losses. This global issue has prompted many scientists to reflect on sustainable solutions using innovative technologies, namely Unmanned Aerial Vehicles (UAV), Machine Learning (ML), and the Internet of Things (IoT). This article aims to present an overview of the use of these new technologies in the analysis of the water status of crops for better irrigation management, with an emphasis on arboriculture. The review demonstrated the importance of UAV-ML-IoT technologies. This contribution is due to the relevant information that can be collected from IoT sensors and extracted from UAV images through various sensors (RGB, multispectral, hyperspectral, thermal), and the ability of ML models to monitor and predict water status. The review in this paper is organized into four main sections: the use of UAV in arboriculture, UAV for irrigation management in arboriculture, IoT systems and irrigation management, and ML for data processing and decision-making. A discussion is presented regarding the prospects for smart irrigation using geospatial technologies and machine learning. Full article

Understanding the nature of the perceived quality advantage of organically-grown early crop potatoes over conventionally-grown ones is of relevance given the expansion in demand for foodstuffs produced by environmentally friendly agricultural practices. The effect of the cultivation system (organic vs. conventional) on physicochemical (skin color, firmness, skin thickness, pH, titratable acidity), nutritional (dry matter, ascorbic acid, total phenolics content, antioxidant activity), and sensorial (for boiled and fried tubers) traits of early potatoes were explored in a field trial conducted during two-seasons in Sicily (Southern Italy) and involving five yellow-fleshed genotypes. The organic cultivation system, averaged across seasons and genotypes, produced tubers displaying a more attractive skin color, with higher skin thickness and firmness, higher dry matter content (19.0 vs. 17.9%), and total phenolics content (350 vs. 232 mg GAE 100 g⁻¹ dry weight) but lower ascorbic acid content (76 vs. 103 mg 100 g⁻¹ dry weight) and antioxidant activity (42 vs. 56% DPPH reduction). The organic cultivation did not affect attributes after boiling but improved all sensory attributes (crispness, typical taste, and browning degree) after frying, highlighting that the superiority of the organic potatoes does not cover all aspects of quality. The positive effects of organic cultivation on physicochemical, nutritional, and sensorial quality were particularly evident in Arinda, Ditta, and ISCI 4F88. Even if the response of organic cultivation on overall quality also depended upon seasonal conditions, cultivar choice plays a key role in optimizing this production system, highlighting the importance of breeding programs. Full article

Fusarium head blight and the contamination of cereals with toxic fungal metabolites are particularly important problems in global agriculture. The increasingly frequent isolation of F. equiseti from cereal grain and the sparse information in the literature on the harmfulness of this fungus to oat encouraged us to conduct the present research. The aim of the study was to determine the susceptibility of oat genotypes to panicle infection by F. equiseti and mycotoxin content in the grain. Field experiments involving 10 oat genotypes were conducted over three years (2015–2017). Oat panicles were inoculated with a conidial suspension of F. equiseti, which reduced the kernels yield by 38.34%, the number of kernels per panicle by 31.16% and 1000 kernels weight by 12.66%. F. equiseti accumulated type A trichothecenes (T-2 and HT-2 toxins, scirpentriol, diacetoxyscirpenol, T-2 triol, T-2 tetraol) and type B trichothecenes (deoxynivalenol, 3Ac-DON, 15Ac-DON, nivalenol, fusarenone X) in kernels at an average level of 0.0616 and 0.2035 mg·kg⁻¹, respectively. The highest susceptibility to scabs caused by F. equiseti was found for genotype POB 4901/10, whereas cv. Elegant exhibited the highest resistance to F. equiseti in terms of yield reduction after inoculation. Full article

The application of nitrification inhibitors (NIs) based on ammonium (NH₄⁺) is considered to be an efficient way to reduce nitrogen (N) loss by delaying the nitrification process through influencing ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). However, the inhibition mechanisms of NIs on AOA and AOB in different soils remain unclear. Hence, we explored the two commonly used NIs (3, 4-dimethylepyrazole phosphate (DMPP) and dicyandiamide (DCD) and their combination (DMPP + DCD) on the soil nitrification and abundance of ammonia oxidizers (AOA and AOB) in three black soils (HLJ, NA, and DA) with different physicochemical properties using a 120-day incubation experiment. The results demonstrated that NIs significantly increased NH₄⁺-N concentrations and decreased NO₃⁻-N concentrations in all three tested soils. There was no significant difference in inhibiting nitrification in HLJ among all NI treatments, while DCD was more efficient in NA, DMPP + DCD had better efficiency in DA. The potential nitrification rate (PNR) was greatly decreased by NIs addition, and PNR was significantly positively correlated with AOB (p < 0.05). AOA was dominant in the acid soil. All NI treatments significantly inhibited soil nitrification through inhibiting the growth of AOB in the two soils with higher pH. The abundance of AOA and AOB was significantly correlated with different soil types (positively correlated with soil pH, and negatively correlated with organic matter). Moreover, soil pH and soil organic matter were considered to be the most important factors influencing the inhibition efficiency of NIs and the abundance of AOA and AOB. The application of the NIs combination (DMPP + DCD) was considered to be the most cost-effective way to inhibit soil nitrification in soil with higher pH and lower SOM, which provides a theoretical basis for a field experiment. Full article

Although annual return of litter occurs in tea (Camellia sinensis L.) plantations, the level of soil organic carbon (SOC) therein remains relatively low. The exact impacts of pruned litter on soil and its association with SOC accumulation in tea plantations remain unclear. In this study, we prepared ¹³C-labeled tea plant material and incubated it with soils collected at a tea plantation. Carbon dioxide (CO₂) efflux and its sources were measured and distinguished based on the ¹³C isotopic method. Soil microbial community and the utilization of litter C were assessed by phospholipid fatty acid (PLFA) analysis combined with a stable isotope probing (SIP) technique. Litter incorporation initially significantly increased CO₂ efflux. The majority of CO₂ production (>90%) arose from native SOC mineralization, which was reflected by a strong positive priming effect (PE) that decreased over time. During the incubation period, β-glucosidase activity significantly decreased in both the control and litter-amended soil. A significant difference in the microbial community was observed between control and litter-amended soil, in which litter incorporation significantly increased the biomass of each microbial group relative to control soil. Based on PLFA-SIP, 78% of the C incorporated into individual microbes was derived from native SOC, while only 22% was derived from litter. Additionally, partial least squares regression path modeling (PLS-PM) revealed that the microbes associated with native SOC mineralization directly affected the changes in SOC (ΔC_SOC+litter), whereas microbes related to litter degradation exhibited an indirect effect on ΔC_SOC+litter by affecting β-glucosidase activity under litter incorporation. Taken together, SOC accumulated less in the tea plantation system despite the annual return of pruned litter to the field. Full article

Municipal wastewater is a valuable source of phosphorus (P) for the production of fertilizing products, such as microalgae (MA), crab carapace material (CCM), P salt produced by chemical leaching of sludge (P salt CL), and sewage sludge ash produced by pyrolysis and the incineration of sludge (SSA PI). This study compares the P availability of these fertilizing products in three planting substrates (S1, S2, and S3) during a four-month growth period of perennial ryegrass. The unfertilized control in substrate S3 had a high and available P that masked the effect of the added fertilizing products. The P salt CL fertilizer exhibited the lowest shoot dry matter in the alkaline S2 substrate. Still, it can be used as a good source of P in both acidic and alkaline substrates, given that its shoot P content was among the highest in all substrates tested. The organic-rich fertilizing products, MA and CCM, are better suited for acidic substrates since a pronounced reduction in the shoot yield and P content was seen in the alkaline S2 substrate. In contrast, for the SSA PI fertilizer, the very small differences in shoot dry matter and P content in S1 compared to S2 indicated that it is suitable for both acidic and alkaline substrates. Four months were needed to observe the maximum shoot yields treated with these P fertilizing products. Considering that the substrate solution P (using Rhizons) for the P salt CL and MA fertilizers proved to be similar to shoot P uptake, Rhizon extraction could be used for assessing P bioavailability. The chemical composition of novel products indicated their potential to deliver not only P, but also other nutrients to plants. However, concentrations of inorganic contaminants in all products, except CCM, pointed out a possibility to pollute the environment by applying these fertilizers. Full article

Soybean is one of the most important oilseed crops worldwide. Fertilization severely restricts the yield potential of soybean in the arid regions of Northwest China. A two-year field experiment was conducted to investigate the effects of fertilization on soybean yield in arid areas under a drip irrigation system. The treatment consisted of 14 fertilizer combinations comprising of four rates each of nitrogen (N) (0, 225, 450, and 675 kg ha⁻¹), phosphorus (P) (0, 135, 270, and 405 kg ha⁻¹), and potassium (K) (0, 75, 150, and 225 kg ha⁻¹). The results revealed that grain yield was more sensitive to N fertilizer than to P and K fertilizers. The P and K fertilizers influenced harvest index and biomass, respectively. The optimized combination of fertilizers for high yield, as well as biological and quality traits was obtained by quadratic polynomial regression analysis. The theoretical grain yields based on the performed statistical calculations and plant biomass were greater than 7.21 tons ha⁻¹ and 16.38 tons ha⁻¹ with 300,000 plants ha⁻¹ and were obtained under a fertilization combination of 411.62–418.39 kg ha⁻¹ N, 153.97−251.03 kg ha⁻¹ P₂O₅, and 117.77−144.73 kg ha⁻¹ K₂O. Thus, our findings will serve as a guideline for an effective fertilizer application in order to achieve a balance between grain yield and plant biomass as well as to contribute to the promotion of large-scale cultivation of soybean under drip irrigation. Full article

Wild cardoon (Cynara cardunculus L.) is a widespread Mediterranean plant that accumulates inulin in its roots. This study aimed to analyze the enzyme systems involved in inulin metabolism in the roots of one Sicilian wild cardoon population in relation to the plant’s growth and development stages. During the winter season, the plant showed slow growth; its biomass was represented mainly by leaves and saccharides were mobilized into its roots. During the spring season, the plant doubled its growth rate and differentiated its reproduction organs as a consequence of the cold conditions. The maximum activities of the 1-SST were recorded in line with the high sucrose and inulin levels in roots, which increased quickly. The increase in the 1-FEH activity suggests that fructan-hydrolyzing activity is associated with the sprouting and elongation of plant stalks. The peak of the invertase activity occurred before the 1-FEH peak. The inulin accumulation in the wild cardoon roots was associated with the plant’s reproduction. Sequential 1-SST and 1-FEH activities and the involvement of invertase and 1-FFT in carbohydrate mobilization, in response to the additional energy demand of the plant for stalk elongation before and for capitula development were observed, along with subsequent grain ripening. Full article

Polyphenols with antioxidant compounds represent an important group of bioactive substances in apple production. The objectives of this study were firstly to measure four parameters of antioxidant capacity (AC) and polyphenol content (AC-FRAP (Ferric Reducing Ability of Plasma), AC-DPPH (2,2-diphenyl-1-picrylhydrol), AC-TEAC (Trolox Equivalent Antioxidant Capacity) and total polyphenol content (TPC)) of four scab-resistant apple cultivars (‘Fujion’, ‘Gaia’, ‘Isaaq’ and ‘Smeralda’) using six extraction methods (water control, pectinase, two approaches using ethanol (EtOH-1 and EtOH-2), methanol (MetOH) and methanol + acetone (MetOH + Acetone), and secondly to provide intercorrelations between antioxidant and polyphenol contents of the four resistant apple cultivars under the six extraction methods. Analysis of variance on the four parameters showed a significant (p = 0.05) effect for extraction methods and cultivars. TPC showed the highest values among the four parameters in all extraction methods and cultivars compared to the other three measurements. The pectinase extraction method showed the highest TPC values for the four cultivars. The EtOH-2 extraction method showed the lowest AC-FRAP value for all cultivars. The EtOH-2 extraction method showed the highest AC-DPPH values, whereas the control method showed the lowest values for the four cultivars. The AC-TEAC values were generally the lowest, ranging between 10.8 and 40.5 mg TE 100 g⁻¹ dry matter, and they showed various effects on extraction methods and cultivars. Correlation and regression analyses of 36 pair-variables showed that two pair-variables (TPC vs. AC-FRAP and AC-TEAC vs. AC-DPPH) were significant for all of the six extraction methods and for all cultivars. In conclusion, the extraction method using pectinase enzyme provided the most stable yield of polyphenol content from apple flesh, as confirmed by the examination of four scab-resistant apple cultivars. Full article

Grazing pasture quality and availability, and grazing animal performance, depend on ecological and weather conditions and grazing management. The latter can be improved by remote monitoring of animals and grazed forage. The aim of this study was to test the ability of a new remote-monitoring system to improve cow and pasture performance. The study used 20 collars for a herd of 40 cows, precision technology to monitor each collared cow’s location and activities 24 h per day, and herd-management system (HMS) software to optimize grazing-land and animal performance. The study covered 4 consecutive years of reproductive cycles and seasonal feed supplements. The selected forage’s metabolizable energy (ME) calculated by the HMS was significantly correlated with the ME calculated by fecal near-infrared spectroscopy analysis (r_p = 0.91, p < 0.001). Cows’ daily activities (walking, grazing, resting, and average daily meal duration), energy balance, and forage quality changed with the seasons, mainly affected by the timing, duration, and volume of precipitation. The HMS well identified sickness events, forage quality and availability, cows’ retained energy, and grazing-land stocking rate (2.9 ha/cow). A significant increase in weaning rate along the 5 years of the study (r_p = 0.921, p < 0.01) was found. Full article

Water scarcity is a major challenge to wheat productivity under changing climate conditions, especially in arid and semi-arid regions. During recent years, different agronomic, physiological and molecular approaches have been used to overcome the problems related to drought stress. Breeding approaches, including conventional and modern breeding, are among the most efficient options to overcome drought stress through the development of new varieties adapted to drought. Growing drought-tolerant wheat genotypes may be a sustainable option to boost wheat productivity under drought stress conditions. Therefore, the present study was conducted with the aim to screen different wheat genotypes based on stress tolerance levels. For this purpose, eleven commonly cultivated wheat genotypes (V₁ = Akbar-2019, V₂ = Ghazi-2019, V₃ = Ujala-2016, V₄ = Zincol-2016, V₅ = Anaj-2017, V₆ = Galaxy-2013, V₇ = Pakistan-2013, V₈ = Seher-2006, V₉ = Lasani-2008, V₁₀ = Faisalabad-2008 and V₁₁ = Millat-2011) were grown in pots filled with soil under well-watered (WW, 70% of field capacity) and water stress (WS, 35% of field capacity) conditions. Treatments were arranged under a completely randomized design (CRD) with three replicates. Data on yield and yield-related traits (tillers/plant, spikelets/spike, grains/spike, 100 grain weight, seed and biological yield) and physio-biochemical (chlorophyll contents, relative water content, membrane stability index, leaf nitrogen, phosphorus, and potassium content) attributes were recorded in this experiment. Our results showed that drought stress significantly affected the morpho-physiological, and biochemical attributes in all tested wheat varieties. Among the genotypes, all traits were found to be significantly (p < 0.05) higher in wheat genotype Faisalabad-2008, including biological yield (9.50 g plant⁻¹) and seed yield (3.39 g plant⁻¹), which was also proven to be more drought tolerant than the other tested genotypes. The higher biological and grain yield of genotype Faisalabad-2008 was mainly attributed to greater numbers of tillers/plant and spikelets/spike compared to the other tested genotypes. The wheat genotype Galaxy-2013 had significantly lower biological (7.43 g plant⁻¹) and seed yield (2.11 g plant⁻¹) than all other tested genotypes, and was classified as a drought-sensitive genotype. For the genotypes, under drought stress, biological and grain yield decreased in the order V₁₀ > V₂ > V₁ > V₄ > V₇ > V₁₁ > V₉ > V₈ > V₃ > V₆. These results suggest that screening for drought-tolerant genotypes may be a more viable option to minimize drought-induced effects on wheat in drought-prone regions. Full article