18 pages, 2191 KiB  
Article
A Hypergraph-Based Blockchain Model and Application in Internet of Things-Enabled Smart Homes
by Chao Qu, Ming Tao and Ruifen Yuan
Sensors 2018, 18(9), 2784; https://doi.org/10.3390/s18092784 - 24 Aug 2018
Cited by 78 | Viewed by 7902
Abstract
With the fast development and expansion of the Internet of Things (IoT), billions of smart devices are being continuously connected, and smart homes, as a typical IoT application, are providing people with various convenient applications, but face security and privacy issues. The idea [...] Read more.
With the fast development and expansion of the Internet of Things (IoT), billions of smart devices are being continuously connected, and smart homes, as a typical IoT application, are providing people with various convenient applications, but face security and privacy issues. The idea of Blockchain (BC) theory has brought about a potential solution to the IoT security problem. The emergence of blockchain technology has brought about a change of decentralized management, providing an effective solution for the protection of network security and privacy. On the other hand, the smart devices in IoT are always lightweight and have less energy and memory. This makes the application of blockchain difficult. Against this background, this paper proposes a blockchain model based on hypergraphs. The aims of this model are to reduce the storage consumption and to solve the additional security issues. In the model, we use the hyperedge as the organization of storage nodes and convert the entire networked data storage into part network storage. We discuss the design of the model and security strategy in detail, introducing some use cases in a smart home network and evaluating the storage performance of the model through simulation experiments and an evaluation of the network. Full article
(This article belongs to the Special Issue Sensor Networks for Collaborative and Secure Internet of Things)
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23 pages, 7370 KiB  
Article
A Homogeneous Breast Phantom Measurement System with an Improved Modified Microwave Imaging Antenna Sensor
by Mohammad Tariqul Islam, Md. Samsuzzaman, Md. Tarikul Islam, Salehin Kibria and Mandeep Jit Singh
Sensors 2018, 18(9), 2962; https://doi.org/10.3390/s18092962 - 5 Sep 2018
Cited by 75 | Viewed by 7015
Abstract
Microwave breast imaging has been reported as having the most potential to become an alternative or additional tool to the existing X-ray mammography technique for detecting breast tumors. Microwave antenna sensor performance plays a significant role in microwave imaging system applications because the [...] Read more.
Microwave breast imaging has been reported as having the most potential to become an alternative or additional tool to the existing X-ray mammography technique for detecting breast tumors. Microwave antenna sensor performance plays a significant role in microwave imaging system applications because the image quality is mostly affected by the microwave antenna sensor array properties like the number of antenna sensors in the array and the size of the antenna sensors. In this paper, a new system for successful early detection of a breast tumor using a balanced slotted antipodal Vivaldi Antenna (BSAVA) sensor is presented. The designed antenna sensor has an overall dimension of 0.401λ × 0.401λ × 0.016λ at the first resonant frequency and operates between 3.01 to 11 GHz under 10 dB. The radiating fins are modified by etching three slots on both fins which increases the operating bandwidth, directionality of radiation pattern, gain and efficiency. The antenna sensor performance of both the frequency domain and time domain scenarios and high-fidelity factor with NFD is also investigated. The antenna sensor can send and receive short electromagnetic pulses in the near field with low loss, little distortion and highly directionality. A realistic homogenous breast phantom is fabricated, and a breast phantom measurement system is developed where a two antennas sensor is placed on the breast model rotated by a mechanical scanner. The tumor response was investigated by analyzing the backscattering signals and successful image construction proves that the proposed microwave antenna sensor can be a suitable candidate for a high-resolution microwave breast imaging system. Full article
(This article belongs to the Special Issue Antenna Technologies for Microwave Sensors)
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30 pages, 877 KiB  
Article
IoT Operating System Based Fuzzy Inference System for Home Energy Management System in Smart Buildings
by Qurat-ul Ain, Sohail Iqbal, Safdar Abbas Khan, Asad Waqar Malik, Iftikhar Ahmad and Nadeem Javaid
Sensors 2018, 18(9), 2802; https://doi.org/10.3390/s18092802 - 25 Aug 2018
Cited by 73 | Viewed by 11820
Abstract
Energy consumption in the residential sector is 25% of all the sectors. The advent of smart appliances and intelligent sensors have increased the realization of home energy management systems. Acquiring balance between energy consumption and user comfort is in the spotlight when the [...] Read more.
Energy consumption in the residential sector is 25% of all the sectors. The advent of smart appliances and intelligent sensors have increased the realization of home energy management systems. Acquiring balance between energy consumption and user comfort is in the spotlight when the performance of the smart home is evaluated. Appliances of heating, ventilation and air conditioning constitute up to 64% of energy consumption in residential buildings. A number of research works have shown that fuzzy logic system integrated with other techniques is used with the main objective of energy consumption minimization. However, user comfort is often sacrificed in these techniques. In this paper, we have proposed a Fuzzy Inference System (FIS) that uses humidity as an additional input parameter in order to maintain the thermostat set-points according to user comfort. Additionally, we have used indoor room temperature variation as a feedback to proposed FIS in order to get the better energy consumption. As the number of rules increase, the task of defining them in FIS becomes time consuming and eventually increases the chance of manual errors. We have also proposed the automatic rule base generation using the combinatorial method. The proposed techniques are evaluated using Mamdani FIS and Sugeno FIS. The proposed method provides a flexible and energy efficient decision-making system that maintains the user thermal comfort with the help of intelligent sensors. The proposed FIS system requires less memory and low processing power along with the use of sensors, making it possible to be used in the IoT operating system e.g., RIOT. Simulation results validate that the proposed technique reduces energy consumption by 28%. Full article
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16 pages, 761 KiB  
Article
Simultaneous Localization and Map Change Update for the High Definition Map-Based Autonomous Driving Car
by Kichun Jo, Chansoo Kim and Myoungho Sunwoo
Sensors 2018, 18(9), 3145; https://doi.org/10.3390/s18093145 - 18 Sep 2018
Cited by 70 | Viewed by 10446
Abstract
High Definition (HD) maps are becoming key elements of the autonomous driving because they can provide information about the surrounding environment of the autonomous car without being affected by the real-time perception limit. To provide the most recent environmental information to the autonomous [...] Read more.
High Definition (HD) maps are becoming key elements of the autonomous driving because they can provide information about the surrounding environment of the autonomous car without being affected by the real-time perception limit. To provide the most recent environmental information to the autonomous driving system, the HD map must maintain up-to-date data by updating changes in the real world. This paper presents a simultaneous localization and map change update (SLAMCU) algorithm to detect and update the HD map changes. A Dempster–Shafer evidence theory is applied to infer the HD map changes based on the evaluation of the HD map feature existence. A Rao–Blackwellized particle filter (RBPF) approach is used to concurrently estimate the vehicle position and update the new map state. The detected and updated map changes by the SLAMCU are reported to the HD map database in order to reflect the changes to the HD map and share the changing information with the other autonomous cars. The SLAMCU was evaluated through experiments using the HD map of traffic signs in the real traffic conditions. Full article
(This article belongs to the Section Physical Sensors)
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22 pages, 35796 KiB  
Article
Flood Detection in Gaofen-3 SAR Images via Fully Convolutional Networks
by Wenchao Kang, Yuming Xiang, Feng Wang, Ling Wan and Hongjian You
Sensors 2018, 18(9), 2915; https://doi.org/10.3390/s18092915 - 2 Sep 2018
Cited by 69 | Viewed by 5883
Abstract
Emergency flood monitoring and rescue need to first detect flood areas. This paper provides a fast and novel flood detection method and applies it to Gaofen-3 SAR images. The fully convolutional network (FCN), a variant of VGG16, is utilized for flood mapping in [...] Read more.
Emergency flood monitoring and rescue need to first detect flood areas. This paper provides a fast and novel flood detection method and applies it to Gaofen-3 SAR images. The fully convolutional network (FCN), a variant of VGG16, is utilized for flood mapping in this paper. Considering the requirement of flood detection, we fine-tune the model to get higher accuracy results with shorter training time and fewer training samples. Compared with state-of-the-art methods, our proposed algorithm not only gives robust and accurate detection results but also significantly reduces the detection time. Full article
(This article belongs to the Special Issue First Experiences with Chinese Gaofen-3 SAR Sensor)
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15 pages, 8988 KiB  
Article
Extraction of High-Precision Urban Impervious Surfaces from Sentinel-2 Multispectral Imagery via Modified Linear Spectral Mixture Analysis
by Rudong Xu, Jin Liu and Jianhui Xu
Sensors 2018, 18(9), 2873; https://doi.org/10.3390/s18092873 - 31 Aug 2018
Cited by 68 | Viewed by 6052
Abstract
This study explores the performance of Sentinel-2A Multispectral Instrument (MSI) imagery for extracting urban impervious surface using a modified linear spectral mixture analysis (MLSMA) method. Sentinel-2A MSI provided 10 m red, green, blue, and near-infrared spectral bands, and 20 m shortwave infrared spectral [...] Read more.
This study explores the performance of Sentinel-2A Multispectral Instrument (MSI) imagery for extracting urban impervious surface using a modified linear spectral mixture analysis (MLSMA) method. Sentinel-2A MSI provided 10 m red, green, blue, and near-infrared spectral bands, and 20 m shortwave infrared spectral bands, which were used to extract impervious surfaces. We aimed to extract urban impervious surfaces at a spatial resolution of 10 m in the main urban area of Guangzhou, China. In MLSMA, a built-up image was first extracted from the normalized difference built-up index (NDBI) using the Otsu’s method; the high-albedo, low-albedo, vegetation, and soil fractions were then estimated using conventional linear spectral mixture analysis (LSMA). The LSMA results were post-processed to extract high-precision impervious surface, vegetation, and soil fractions by integrating the built-up image and the normalized difference vegetation index (NDVI). The performance of MLSMA was evaluated using Landsat 8 Operational Land Imager (OLI) imagery. Experimental results revealed that MLSMA can extract the high-precision impervious surface fraction at 10 m with Sentinel-2A imagery. The 10 m impervious surface map of Sentinel-2A is capable of recovering more detail than the 30 m map of Landsat 8. In the Sentinel-2A impervious surface map, continuous roads and the boundaries of buildings in urban environments were clearly identified. Full article
(This article belongs to the Section Remote Sensors)
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11 pages, 963 KiB  
Article
A Study of Diagnostic Accuracy Using a Chemical Sensor Array and a Machine Learning Technique to Detect Lung Cancer
by Chi-Hsiang Huang, Chian Zeng, Yi-Chia Wang, Hsin-Yi Peng, Chia-Sheng Lin, Che-Jui Chang and Hsiao-Yu Yang
Sensors 2018, 18(9), 2845; https://doi.org/10.3390/s18092845 - 28 Aug 2018
Cited by 67 | Viewed by 7214
Abstract
Lung cancer is the leading cause of cancer death around the world, and lung cancer screening remains challenging. This study aimed to develop a breath test for the detection of lung cancer using a chemical sensor array and a machine learning technique. We [...] Read more.
Lung cancer is the leading cause of cancer death around the world, and lung cancer screening remains challenging. This study aimed to develop a breath test for the detection of lung cancer using a chemical sensor array and a machine learning technique. We conducted a prospective study to enroll lung cancer cases and non-tumour controls between 2016 and 2018 and analysed alveolar air samples using carbon nanotube sensor arrays. A total of 117 cases and 199 controls were enrolled in the study of which 72 subjects were excluded due to having cancer at another site, benign lung tumours, metastatic lung cancer, carcinoma in situ, minimally invasive adenocarcinoma, received chemotherapy or other diseases. Subjects enrolled in 2016 and 2017 were used for the model derivation and internal validation. The model was externally validated in subjects recruited in 2018. The diagnostic accuracy was assessed using the pathological reports as the reference standard. In the external validation, the areas under the receiver operating characteristic curve (AUCs) were 0.91 (95% CI = 0.79–1.00) by linear discriminant analysis and 0.90 (95% CI = 0.80–0.99) by the supportive vector machine technique. The combination of the sensor array technique and machine learning can detect lung cancer with high accuracy. Full article
(This article belongs to the Special Issue Electronic Noses and Their Application)
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17 pages, 3141 KiB  
Article
Deep Learning-Based Caution Area Traffic Prediction with Automatic Identification System Sensor Data
by Kwang-Il Kim and Keon Myung Lee
Sensors 2018, 18(9), 3172; https://doi.org/10.3390/s18093172 - 19 Sep 2018
Cited by 65 | Viewed by 7039
Abstract
In a crowded harbor water area, it is a major concern to control ship traffic for assuring safety and maximizing the efficiency of port operations. Vessel Traffic Service (VTS) operators pay much attention to caution areas like ship route intersections or traffic congestion [...] Read more.
In a crowded harbor water area, it is a major concern to control ship traffic for assuring safety and maximizing the efficiency of port operations. Vessel Traffic Service (VTS) operators pay much attention to caution areas like ship route intersections or traffic congestion area in which there are some risks of ship collision. They want to control the traffic of the caution area at a proper level to lessen risk. Inertial ship movement makes swift changes in direction and speed difficult. It is hence important to predict future traffic of the caution area earlier on so as to get enough time for control actions on ship movements. In the harbor area, VTS stations collect a large volume of Automatic Identification Service (AIS) sensor data, which contain information about ship movement and ship attributes. This paper proposes a new deep neural network model called Ship Traffic Extraction Network (STENet) to predict the medium-term traffic and long-term traffic of the caution area. The STENet model is trained with AIS sensor data. The STENet model is organized into a hierarchical architecture in which the outputs of the movement and contextual feature extraction modules are concatenated and fed into a prediction module. The movement module extracts the features of overall ship movements with a convolutional neural network. The contextual modules consist of five separated fully-connected neural networks, each of which receives an associated attribute. The separation of feature extraction modules at the front phase helps extract the effective features by preventing unrelated attributes from crosstalking. To evaluate the performance of the proposed model, the developed model is applied to a real AIS sensor dataset, which has been collected over two years at a Korean port called Yeosu. In the experiments, four methods have been compared including two new methods: STENet and VGGNet-based models. For the real AIS sensor dataset, the proposed model has shown 50.65% relative performance improvement on average for the medium-term predictions and 57.65% improvement on average for the long-term predictions over the benchmark method, i.e., the SVR-based method. Full article
(This article belongs to the Special Issue Innovative Sensor Technology for Intelligent System and Computing)
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15 pages, 3442 KiB  
Review
Ultra-Wideband (UWB) Antenna Sensor Based Microwave Breast Imaging: A Review
by Md. Zulfiker Mahmud, Mohammad Tariqul Islam, Norbahiah Misran, Ali F. Almutairi and Mengu Cho
Sensors 2018, 18(9), 2951; https://doi.org/10.3390/s18092951 - 5 Sep 2018
Cited by 65 | Viewed by 9828
Abstract
Globally, breast cancer is reported as a primary cause of death in women. More than 1.8 million new breast cancer cases are diagnosed every year. Because of the current limitations on clinical imaging, researchers are motivated to investigate complementary tools and alternatives to [...] Read more.
Globally, breast cancer is reported as a primary cause of death in women. More than 1.8 million new breast cancer cases are diagnosed every year. Because of the current limitations on clinical imaging, researchers are motivated to investigate complementary tools and alternatives to available techniques for detecting breast cancer in earlier stages. This article presents a review of concepts and electromagnetic techniques for microwave breast imaging. More specifically, this work reviews ultra-wideband (UWB) antenna sensors and their current applications in medical imaging, leading to breast imaging. We review the use of UWB sensor based microwave energy in various imaging applications for breast tumor related diseases, tumor detection, and breast tumor detection. In microwave imaging, the back-scattered signals radiating by sensors from a human body are analyzed for changes in the electrical properties of tissues. Tumorous cells exhibit higher dielectric constants because of their high water content. The goal of this article is to provide microwave researchers with in-depth information on electromagnetic techniques for microwave imaging sensors and describe recent developments in these techniques. Full article
(This article belongs to the Special Issue Antenna Technologies for Microwave Sensors)
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13 pages, 2639 KiB  
Article
Pixel-Wise Crack Detection Using Deep Local Pattern Predictor for Robot Application
by Yundong Li, Hongguang Li and Hongren Wang
Sensors 2018, 18(9), 3042; https://doi.org/10.3390/s18093042 - 11 Sep 2018
Cited by 64 | Viewed by 5962
Abstract
Robotic vision-based crack detection in concrete bridges is an essential task to preserve these assets and their safety. The conventional human visual inspection method is time consuming and cost inefficient. In this paper, we propose a robust algorithm to detect cracks in a [...] Read more.
Robotic vision-based crack detection in concrete bridges is an essential task to preserve these assets and their safety. The conventional human visual inspection method is time consuming and cost inefficient. In this paper, we propose a robust algorithm to detect cracks in a pixel-wise manner from real concrete surface images. In practice, crack detection remains challenging in the following aspects: (1) detection performance is disturbed by noises and clutters of environment; and (2) the requirement of high pixel-wise accuracy is difficult to obtain. To address these limitations, three steps are considered in the proposed scheme. First, a local pattern predictor (LPP) is constructed using convolutional neural networks (CNN), which can extract discriminative features of images. Second, each pixel is efficiently classified into crack categories or non-crack categories by LPP, using as context a patch centered on the pixel. Lastly, the output of CNN—i.e., confidence map—is post-processed to obtain the crack areas. We evaluate the proposed algorithm on samples captured from several concrete bridges. The experimental results demonstrate the good performance of the proposed method. Full article
(This article belongs to the Special Issue Semantic Representations for Behavior Analysis in Robotic System)
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18 pages, 3612 KiB  
Article
Evaluating RGB Imaging and Multispectral Active and Hyperspectral Passive Sensing for Assessing Early Plant Vigor in Winter Wheat
by Lukas Prey, Malte Von Bloh and Urs Schmidhalter
Sensors 2018, 18(9), 2931; https://doi.org/10.3390/s18092931 - 3 Sep 2018
Cited by 63 | Viewed by 6763
Abstract
Plant vigor is an important trait of field crops at early growth stages, influencing weed suppression, nutrient and water use efficiency and plant growth. High-throughput techniques for its evaluation are required and are promising for nutrient management in early growth stages and for [...] Read more.
Plant vigor is an important trait of field crops at early growth stages, influencing weed suppression, nutrient and water use efficiency and plant growth. High-throughput techniques for its evaluation are required and are promising for nutrient management in early growth stages and for detecting promising breeding material in plant phenotyping. However, spectral sensing for assessing early plant vigor in crops is limited by the strong soil background reflection. Digital imaging may provide a low-cost, easy-to-use alternative. Therefore, image segmentation for retrieving canopy cover was applied in a trial with three cultivars of winter wheat (Triticum aestivum L.) grown under two nitrogen regimes and in three sowing densities during four early plant growth stages (Zadok’s stages 14–32) in 2017. Imaging-based canopy cover was tested in correlation analysis for estimating dry weight, nitrogen uptake and nitrogen content. An active Greenseeker sensor and various established and newly developed vegetation indices and spectral unmixing from a passive hyperspectral spectrometer were used as alternative approaches and additionally tested for retrieving canopy cover. Before tillering (until Zadok’s stage 20), correlation coefficients for dry weight and nitrogen uptake with canopy cover strongly exceeded all other methods and remained on higher levels (R² > 0.60***) than from the Greenseeker measurements until tillering. From early tillering on, red edge based indices such as the NDRE and a newly extracted normalized difference index (736 nm; ~794 nm) were identified as best spectral methods for both traits whereas the Greenseeker and spectral unmixing correlated best with canopy cover. RGB-segmentation could be used as simple low-cost approach for very early growth stages until early tillering whereas the application of multispectral sensors should consider red edge bands for subsequent stages. Full article
(This article belongs to the Special Issue Advanced Sensor Technologies for Crop Phenotyping Application)
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15 pages, 3676 KiB  
Article
DM-MQTT: An Efficient MQTT Based on SDN Multicast for Massive IoT Communications
by Jun-Hong Park, Hyeong-Su Kim and Won-Tae Kim
Sensors 2018, 18(9), 3071; https://doi.org/10.3390/s18093071 - 12 Sep 2018
Cited by 61 | Viewed by 11668
Abstract
Edge computing is proposed to solve the problem of centralized cloud computing caused by a large number of IoT (Internet of Things) devices. The IoT protocols need to be modified according to the edge computing paradigm, where the edge computing devices for analyzing [...] Read more.
Edge computing is proposed to solve the problem of centralized cloud computing caused by a large number of IoT (Internet of Things) devices. The IoT protocols need to be modified according to the edge computing paradigm, where the edge computing devices for analyzing IoT data are distributed to the edge networks. The MQTT (Message Queuing Telemetry Transport) protocol, as a data distribution protocol widely adopted in many international IoT standards, is suitable for cloud computing because it uses a centralized broker to effectively collect and transmit data. However, the standard MQTT may suffer from serious traffic congestion problem on the broker, causing long transfer delays if there are massive IoT devices connected to the broker. In addition, the big data exchange between the IoT devices and the broker decreases network capability of the edge networks. The authors in this paper propose a novel MQTT with a multicast mechanism to minimize data transfer delay and network usage for the massive IoT communications. The proposed MQTT reduces data transfer delays by establishing bidirectional SDN (Software Defined Networking) multicast trees between the publishers and the subscribers by means of bypassing the centralized broker. As a result, it can reduce transmission delay by 65% and network usage by 58% compared with the standard MQTT. Full article
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29 pages, 17384 KiB  
Article
Radar and Visual Odometry Integrated System Aided Navigation for UAVS in GNSS Denied Environment
by Mostafa Mostafa, Shady Zahran, Adel Moussa, Naser El-Sheimy and Abu Sesay
Sensors 2018, 18(9), 2776; https://doi.org/10.3390/s18092776 - 23 Aug 2018
Cited by 60 | Viewed by 7918
Abstract
Drones are becoming increasingly significant for vast applications, such as firefighting, and rescue. While flying in challenging environments, reliable Global Navigation Satellite System (GNSS) measurements cannot be guaranteed all the time, and the Inertial Navigation System (INS) navigation solution will deteriorate dramatically. Although [...] Read more.
Drones are becoming increasingly significant for vast applications, such as firefighting, and rescue. While flying in challenging environments, reliable Global Navigation Satellite System (GNSS) measurements cannot be guaranteed all the time, and the Inertial Navigation System (INS) navigation solution will deteriorate dramatically. Although different aiding sensors, such as cameras, are proposed to reduce the effect of these drift errors, the positioning accuracy by using these techniques is still affected by some challenges, such as the lack of the observed features, inconsistent matches, illumination, and environmental conditions. This paper presents an integrated navigation system for Unmanned Aerial Vehicles (UAVs) in GNSS denied environments based on a Radar Odometry (RO) and an enhanced Visual Odometry (VO) to handle such challenges since the radar is immune against these issues. The estimated forward velocities of a vehicle from both the RO and the enhanced VO are fused with the Inertial Measurement Unit (IMU), barometer, and magnetometer measurements via an Extended Kalman Filter (EKF) to enhance the navigation accuracy during GNSS signal outages. The RO and VO are integrated into one integrated system to help overcome their limitations, since the RO measurements are affected while flying over non-flat terrain. Therefore, the integration of the VO is important in such scenarios. The experimental results demonstrate the proposed system’s ability to significantly enhance the 3D positioning accuracy during the GNSS signal outage. Full article
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21 pages, 7598 KiB  
Article
Non-Contact Body Measurement for Qinchuan Cattle with LiDAR Sensor
by Lvwen Huang, Shuqin Li, Anqi Zhu, Xinyun Fan, Chenyang Zhang and Hongyan Wang
Sensors 2018, 18(9), 3014; https://doi.org/10.3390/s18093014 - 9 Sep 2018
Cited by 59 | Viewed by 16540
Abstract
The body dimension measurement of large animals plays a significant role in quality improvement and genetic breeding, and the non-contact measurements by computer vision-based remote sensing could represent great progress in the case of dangerous stress responses and time-costing manual measurements. This paper [...] Read more.
The body dimension measurement of large animals plays a significant role in quality improvement and genetic breeding, and the non-contact measurements by computer vision-based remote sensing could represent great progress in the case of dangerous stress responses and time-costing manual measurements. This paper presents a novel approach for three-dimensional digital modeling of live adult Qinchuan cattle for body size measurement. On the basis of capturing the original point data series of live cattle by a Light Detection and Ranging (LiDAR) sensor, the conditional, statistical outliers and voxel grid filtering methods are fused to cancel the background and outliers. After the segmentation of K-means clustering extraction and the RANdom SAmple Consensus (RANSAC) algorithm, the Fast Point Feature Histogram (FPFH) is put forward to get the cattle data automatically. The cattle surface is reconstructed to get the 3D cattle model using fast Iterative Closest Point (ICP) matching with Bi-directional Random K-D Trees and a Greedy Projection Triangulation (GPT) reconstruction method by which the feature points of cattle silhouettes could be clicked and calculated. Finally, the five body parameters (withers height, chest depth, back height, body length, and waist height) are measured in the field and verified within an accuracy of 2 mm and an error close to 2%. The experimental results show that this approach could be considered as a new feasible method towards the non-contact body measurement for large physique livestock. Full article
(This article belongs to the Special Issue Sensors in Agriculture 2018)
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13 pages, 1722 KiB  
Article
Molecular Fingerprints of Hemoglobin on a Nanofilm Chip
by Yeşeren Saylan and Adil Denizli
Sensors 2018, 18(9), 3016; https://doi.org/10.3390/s18093016 - 9 Sep 2018
Cited by 58 | Viewed by 5300
Abstract
Hemoglobin is an iron carrying protein in erythrocytes and also an essential element to transfer oxygen from the lungs to the tissues. Abnormalities in hemoglobin concentration are closely correlated with health status and many diseases, including thalassemia, anemia, leukemia, heart disease, and excessive [...] Read more.
Hemoglobin is an iron carrying protein in erythrocytes and also an essential element to transfer oxygen from the lungs to the tissues. Abnormalities in hemoglobin concentration are closely correlated with health status and many diseases, including thalassemia, anemia, leukemia, heart disease, and excessive loss of blood. Particularly in resource-constrained settings existing blood analyzers are not readily applicable due to the need for high-level instrumentation and skilled personnel, thereby inexpensive, easy-to-use, and reliable detection methods are needed. Herein, a molecular fingerprints of hemoglobin on a nanofilm chip was obtained for real-time, sensitive, and selective hemoglobin detection using a surface plasmon resonance system. Briefly, through the photopolymerization technique, a template (hemoglobin) was imprinted on a monomeric (acrylamide) nanofilm on-chip using a cross-linker (methylenebisacrylamide) and an initiator-activator pair (ammonium persulfate-tetramethylethylenediamine). The molecularly imprinted nanofilm on-chip was characterized by atomic force microscopy and ellipsometry, followed by benchmarking detection performance of hemoglobin concentrations from 0.0005 mg mL−1 to 1.0 mg mL−1. Theoretical calculations and real-time detection implied that the molecularly imprinted nanofilm on-chip was able to detect as little as 0.00035 mg mL−1 of hemoglobin. In addition, the experimental results of hemoglobin detection on the chip well-fitted with the Langmuir adsorption isotherm model with high correlation coefficient (0.99) and association and dissociation coefficients (39.1 mL mg−1 and 0.03 mg mL−1) suggesting a monolayer binding characteristic. Assessments on selectivity, reusability and storage stability indicated that the presented chip is an alternative approach to current hemoglobin-targeted assays in low-resource regions, as well as antibody-based detection procedures in the field. In the future, this molecularly imprinted nanofilm on-chip can easily be integrated with portable plasmonic detectors, improving its access to these regions, as well as it can be tailored to detect other proteins and biomarkers. Full article
(This article belongs to the Special Issue Imprinting Technology for Advanced Point-of-Care Sensing)
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