Search Results (265)

Finding eco-friendly alternatives to the synthetic materials used for acoustic application in building industry is necessary to address environmental sustainability. Biocomposites of natural fibers combined with a biopolymer matrix emerge as a promising approach. In this study, soy protein biocomposites were prepared with 10, 15, and 20 wt% sheep wool and were added spent coffee grounds by freeze-drying to create fibro-porous biocomposites for acoustic applications. Transmission loss (TL) measurements underlined good behavior as sound insulators, with maximum values around 22 dB at 2500 Hz and even better performance than those of commercial synthetic solutions. The obtained sound absorption coefficients were competitive, as they almost reached unity at medium and high frequencies. Airflow resistivity was determined, and values were higher for the biocomposites with coffee grounds, specifically 14–18 kPa·s·m⁻² vs. 5.62–11.6 kPa·s·m⁻². Using the input of the measured airflow resistivity, an empirical model using a genetic algorithm was developed as a prediction tool for the sound absorption performance of the samples. All in all, results showcase the feasibility of employing the studied biocomposites as competitive sound insulators and absorbers in building construction industry. Full article

The growing amount of waste worldwide requires new solutions for its management. Agricultural by-products account for almost 10% of the waste generated. One of them is sheep wool, a natural fibre with beneficial physicochemical properties. Currently, sheep wool production amounts to approximately 1–2 million tonnes per year, of which 60% is used in the manufacture of clothing. Nevertheless, it poses a considerable challenge in terms of disposal due to its keratin-rich composition and slow biodegradability. This review analyses the chemical and physical properties of sheep wool and assesses its potential as biomass based on its carbon content and other elemental components. This allows us to provide a critical comparative analysis of the main technological pathways for the use of waste sheep wool as biomass, including anaerobic digestion, pyrolysis, direct combustion and gasification. The review highlights both the opportunities and limitations of these processes, comparing sheep wool in terms of energy potential and carbon footprint with other biomass. The review shows that the calorific value of sheep wool (19.5 MJ/kg) is competitive with traditional plant-based biofuels and the use of waste sheep wool as biomass source can contribute to reduction in CO₂ emissions of 2.1 million tonnes per year. The use of sheep wool as biomass can not only contribute to waste reduction but also supports the goals of sustainable agriculture and climate neutrality. The selected methods may offer a new and effective way of reducing waste and allow all sheep wool produced to be introduced into the circular economy. Full article

Keratinous biomass, such as feathers, wool, and hair, poses environmental challenges due to its insoluble and recalcitrant nature. In this study, we identified, purified and comprehensively characterized a previously uncharacterized extracellular alkaline keratinase, KerFJ, secreted by Bacillus sp. FJ-3-16, with broad industrial application potential. KerFJ was produced at high yield (1800 U/mL) in an optimized cost-effective medium and purified to homogeneity using ion-exchange chromatography. The enzyme exhibited optimal activity at pH 9.5 and 55 °C, with remarkable alkaline and thermal stability, and high tolerance to surfactants, oxidants, and metal ions. Sequence analysis revealed that KerFJ is a member of the serine peptidase S8 family, with a molecular weight of ~27.5 kDa. It efficiently degraded native keratin substrates, achieving 70.3 ± 2.1% feather, 39.7 ± 1.8% wool, and 15.4 ± 1.2% hair degradation, and the resulting feather hydrolysates exhibited strong antioxidant activities. KerFJ also demonstrated excellent compatibility with commercial detergents and enabled effective stain removal from fabrics without damage. Moreover, both laboratory- and pilot-scale trials showed that KerFJ facilitated non-destructive dehairing of sheep, donkey, and pig skins while preserving collagen integrity. These results highlight KerFJ as a robust and multifunctional biocatalyst suitable for keratin waste valorization, eco-friendly leather processing, and detergent formulations. Full article

To identify molecular markers associated with wool traits in fine-wool sheep, we examined genetic polymorphisms in the NOTCH2 and CD1A genes in 944 Subo Merino sheep in this study. Subsequently, we performed association analyses between mutation sites in the NOTCH2 and CD1A genes and wool traits using SAS 9.4 software, followed by linkage disequilibrium (LD) analysis of different mutation sites using Haploview 4.2 software. Additionally, bioinformatics tools were employed to predict the potential impacts of missense mutations on protein secondary and tertiary structures. Finally, quantitative PCR (qPCR) was used to assess the expression levels of the NOTCH2 and CD1A genes. Genetic analysis revealed six polymorphic sites in NOTCH2 and CD1A, all of which were missense mutations. Two SNPs in NOTCH2 (SNP1 and SNP2) showed significant associations with the coefficient of variation of fibre diameter, and SNP1 was also associated with greasy fleece weight. Four SNPs in CD1A (SNP3–SNP6) were significantly associated with fibre diameter standard deviation, and SNP3, SNP4, and SNP5 were additionally associated with crimp number. LD analysis revealed that SNP3, SNP4, and SNP5 were closely linked. Bioinformatics analysis indicated that the mutations caused alterations in the secondary and tertiary structures of the NOTCH2 and CD1A proteins. qPCR results showed that the CD1A gene was highly expressed in the fine wool fibre group compared with the ultra-fine wool fibre group. In conclusion, this study revealed a genetic association between NOTCH2 and CD1A and wool traits. The results are expected to provide a theoretical foundation for breeding wool traits in Subo Merino sheep, thereby enhancing the economic value of fine wool. Full article

The wool of sheep consists of structurally intricate natural fibres that can be processed and manufactured into a range of products. It is prized for its insulation, moisture-buffering capability, flame resistance, and biodegradability. These features arise from its unique fibre architecture and specialised protein composition, which set it apart from most other natural and synthetic fibres. However, despite these novel characteristics, wool fibre variation hampers its uses and reduces its ability to compete with other fibres. This review summarises our current knowledge of wool fibre biology. It begins with a description of wool’s functional properties and performance attributes, then explores the structural foundations of these properties, the molecular basis of fibre trait variation, and prospects for improving fibre quality using genetic approaches. Particular attention is given to the wool keratin and keratin-associated protein genes, their spatiotemporal expression patterns, and genetic polymorphism that may influence fibre characteristics. Opportunities for the genetic improvement of sheep are discussed, including the use of genetic modification and marker-assisted selection. Challenges in interpreting gene–trait associations, particularly from high-throughput omics studies, are highlighted, along with the need for functionally validated genetic markers. Potential trade-offs between wool characteristics and other production and reproductive traits are considered, emphasising the need for balanced breeding approaches. By integrating insights from structural biology, molecular genetics, and breeding strategies, this review provides a foundation for wool fibre improvement. Full article

The keratin-associated proteins (KAPs) are a class of wool proteins. They form a matrix that cross-links the wool intermediate filament keratins. The KAPs are thought to affect wool fibre structure and properties and have been associated with variation in wool fibre traits. There are many KAP genes in sheep, but not all have been identified. Recently a second member of the KAP22 gene family, KRTAP22-2, was identified in goats, and variation in this caprine gene was associated with cashmere fibre traits. In this study, we identified ovine KRTAP22-2. To ascertain the extent of variation in KRTAP22-2, sheep from eight breeds were investigated using polymerase chain reaction (PCR) followed by single-strand conformational polymorphism (SSCP) analysis. This revealed two unique banding patterns, which upon sequencing gave two novel DNA sequences. These differed by two single nucleotide polymorphisms in the coding region. Three genotypes of the novel KRTAP22-2 sequences were observed in the eight sheep breeds studied. The ovine KRTAP22-2 variant sequences were similar to a goat KRTAP22-2 variant, but a search of ovine expressed sequence tags revealed no matching mRNA sequences in the ovine databases. In a second part of the study, no association was found between the KRTAP22-2 genotypes and mean fibre diameter, fibre diameter standard deviation, coefficient of variation in fibre diameter, and mean fibre curvature, for either the fine wool or heterotypic hair fibres of 255 Chinese Tan lambs. These results suggests that sheep have a KRTAP22-2 gene, but that there may be species-specific differences in the gene’s expression or function. The gene may not affect wool traits in the way that it appears to in goats. Full article

Sheep breeding is an important sector of livestock production in the Republic of Kazakhstan. The Kazakh fat-tailed coarse-wool sheep holds a prominent position among local breeds due to its high meat productivity, resilience to extreme climatic conditions, and efficient use of pasture resources. This study focuses on the analysis of runs of homozygosity (ROH) to evaluate the genetic diversity level, inbreeding and to detect selection signatures in the Kazakh fat-tailed coarse-wool sheep breed. A total of 500 animals were genotyped using the OvineSNP50 BeadChip (Illumina, San Diego, CA, USA). As a result, a total of 41,728 ROH segments were identified, with an average length of 1.59 Mb, distributed across the entire genome. The most prominent homozygous regions were detected on chromosomes OAR10, OAR13, and OAR22, which might be associated with selection signatures. Genomic inbreeding coefficients (F_ROH and F_GRM) showed a strong positive correlation (r = 0.58, p < 0.001), supporting the effectiveness of ROH-based analysis. Several candidate genes were detected, including MYF5, PRDM16, TGM3, SLC26A4 and SMAD5 which are notably involved in muscle formation, wool traits, and fat metabolism. The findings have substantial practical value for breeding programs and for managing genetic diversity in sheep farming enterprises in the Republic of Kazakhstan. Full article

Dorper sheep (DOR) are a commercially important mutton breed renowned for their high growth rate, favorable carcass composition, environmental adaptability, and natural wool shedding. In China, they are widely utilized as terminal sires to enhance growth and carcass yield in local breeds. To elucidate the genetic basis of these traits, we sequenced the genomes of 20 DOR and integrated the data with whole-genome sequences from 73 individuals representing four Chinese indigenous breeds. Analyses of genetic diversity, inbreeding coefficients, and population structure revealed reduced genomic diversity, elevated inbreeding levels, and clear genetic separation for DOR from other indigenous breeds. Selective sweep scans using F_ST, pi, and XP-EHH identified candidate genes involved in five major trait categories: growth performance and development (COL2A1, DAB2IP, EPYC, TSPAN18, WNT1, CTPS1, FBXW7, INSR, S100A6, SOCS2), energy metabolism (ACSS3, ADGRE3, CPT2, GCGR, PRKAA1), fat deposition and adipocyte differentiation (EHBP1, FOXP1, KLF12, PDGFD, RALGAPA2), immune response (CXCR6, IL17RB, NFKBIZ, TMEM154), and wool traits (CERS4, MITF). These results will provide novel insights into the genomic architecture of economically important traits in DOR and support their genetic improvement through informed crossbreeding with Chinese local breeds. Full article

Various signalling molecules and pathways critical for wool production and quality regulate wool secondary follicle (SF) development. Circular RNAs (circRNAs) regulate SF morphogenesis through a competing endogenous RNA (ceRNA) mechanism; these novel cyclic non-coding RNAs are not known to regulate the development of SFs in the foetal period of fine-wool sheep. Here, we analysed circRNA expression profiles in the foetal skin of Gansu Alpine fine-wool sheep at 10 developmental stages (E87, E90, E93, E96, E99, E102, E105, E108, E111, and E138) using RNA sequencing. Among the differentially expressed circRNAs (DE circRNAs), 173 were significantly enriched in signalling pathways related to hair follicle (HF) development, such as Wnt/β-catenin, transforming growth factor-β/Smad, Notch, and mitogen-activated protein kinase. Six HF-development-related circRNAs were expressed at different stages and potentially regulated SF development through the ceRNA network. In total, 16 DE circRNAs and their targets, 44 miRNAs, and 65 mRNAs were screened, and 88 ceRNA regulatory network pathways related to SF development were constructed. These key DE circRNAs could be candidate genes for further exploration of the molecular HF development mechanism, providing an important theoretical basis for unravelling the regulatory network of SF development in fine-wool sheep and genetic wool trait improvement. Full article

Zootechnical practices such as tail docking are still in use in dairy sheep farming, performed in the first week of life, mainly by rubber ring and only rarely by surgical methods. In this study, we evaluated the impact of caudectomy on ovine stress levels, inflammation, and health status by comparing tail docking carried out using rubber rings or surgical amputation. Twenty-one lambs were randomly selected and equally allocated into three groups: controls (n = 7), lambs with tail cut by rubber rings (n = 7), and lambs with caudectomy performed by surgical practice (n = 7). Several biochemical parameters and inflammatory markers were monitored at different times post-caudectomy, as well as wool levels of the stress marker cortisol. Our data revealed that lambs that underwent tail docking by rubber rings, but not by surgical procedure, presented inflammation and stress, as well as a moderate increase in muscular damage markers. These results are useful for the evaluation of animal welfare in dairy sheep that underwent caudectomy, highlighting the need to re-evaluate this procedure, as well as the ways in which it is performed. Full article

Fiber fineness is a critical determinant of wool quality and is of great significance in enhancing the overall quality of wool. The aim of this research was to pinpoint the key proteins that participate in the regulation of wool fineness. To achieve this, we utilized Astral—DIA proteomics technology to examine the disparities in proteins, pathways, and GO terms among the wool tissues of Gansu alpine fine-wool sheep with varying mean fiber diameters (MFD). The experiment was divided into two groups: coarse (group C, MFD = 22.36 ± 0.75 μm, n = 4) and fine (group F, MFD = 16.89 ± 0.36 μm, n = 4). The results indicated that 67 differentially expressed proteins (DEPs) were identified from the wool tissues of Gansu alpine fine-wool sheep in groups C and F. Functional enrichment analysis demonstrated that several key differential proteins, including MGST3, KRT26, KRT72, KRT74, KRT71, etc., were mainly enriched in multiple functional pathways. These pathways included glutathione metabolism, oxidative phosphorylation, the degradation of valine, leucine, and isoleucine, intermediate filaments, serine protease activity, and cysteine protease activity (p < 0.05). Furthermore, protein–protein interaction (PPI) network analysis suggested that type II keratin and type I keratin (such as CTSF, PSAP, TMEM106B, LYPD3, KRT71, KRT72), along with glutathione metabolism (MGST3, W5QDB7), are closely related to hair follicle development and the regulation of wool fineness. In summary, this study enriches the existing sheep proteinome database and offers novel perspectives on the regulatory mechanisms of wool fineness. Full article

Background: In Kazakhstan, there is a notable demand for fat-tail sheep breeds in both domestic and international markets, which has led to the prioritization of certain breeds for breeding purposes. Among the various sheep breeds raised in the desert and semi-desert regions of Kazakhstan, the Kazakh fat-tailed coarse-wool sheep is particularly valued for its production of high-quality mutton. Objective: This study aimed to identify genomic regions and candidate genes associated with body conformation traits in this breed using a genome-wide association study (GWAS). Methods: A GWAS was performed on 295 Kazakh fat-tailed coarse-wool using OvineSNP50 Genotyping BeadChip (Illumina, San Diego, CA, USA). Results: After quality control, 41,912 single-nucleotide polymorphisms (SNPs) remained for analysis. Several loci showed suggestive associations (p < 1 × 10⁻⁵) with growth traits. These included s23127.1 and OAR6_56152225.1 for live weight; s08490.1 for chest width; s22731.1 for oblique length; OAR10_1168444.1 for cannon bone circumference; and s58409.1 for both rump height and withers height. Candidate genes near these loci encompassed VCAN, NEK1, NRG1, ADAM12, ERBB4, RUNX1T1, and PDGFD. Conclusion: Thus, these genetic variations have the potential to serve as candidate markers for MAS targeting body conformation traits in Kazakh fat-tailed coarse-wool sheep. Full article

In this study, 944 Subo Merino sheep, a high-quality fine wool breed, were selected as research subjects. The SNP typing of the FAT3 gene was performed using the Fluidigm BiomarkTM HD system, and 11 missense mutation sites were identified. The analysis of population polymorphism of single-nucleotide polymorphisms was conducted. It is noteworthy that a substantial strong linkage disequilibrium was identified between SNP 5 and SNP 6 (r² > 0.8). The association between SNPs of the FAT3 gene and wool traits showed that multiple SNPs were significantly correlated with several different wool traits (p < 0.05). Furthermore, the investigation delved into the impact of the FAT3 gene on wool fiber through the utilization of quantitative polymerase chain reaction (qPCR), which yielded findings that this gene was notably expressed in fine wool fiber (FW) (p < 0.001). To predict the subcellular localization and protein transmembrane structure of FAT3, we employed the PSORT II Prediction and TMHMM online software. It was determined that the protein contains a transmembrane domain. This study provides molecular markers for the improvement of the selection and breeding of ultrafine-wool sheep and offers experimental evidence for accelerating the genetic breeding of sheep. Full article

Conventional protective coatings based on petroleum raw materials have certain limitations in terms of their availability, environmental pollution, and sustainability. Therefore, this research successfully investigates the potential of sheep wool-derived biochar to develop a sustainable, high-performance protective coating. Two variants of biochar, namely SW800 and SW1000, were developed by pyrolyzing sheep wool at 800 °C and at 1000 °C for 1 h, respectively. The prepared samples were characterized using FTIR, FESEM-EDX, and XRD analyses to confirm the structural and elemental differences between both biochar samples. Furthermore, biochar-based epoxy coatings were developed by varying the concentration of prepared biochar from 1% to 5%. The coating performance was evaluated for its aesthetic, mechanical, chemical resistance, and hydrophobicity. Crucially, this study demonstrated that biochar inclusion did not compromise critical mechanical and chemical properties like adhesion (5B), flexibility (7 mm), scratch hardness (3500 gms), pencil hardness (3H), acid-alkali resistance, and solvent rub test (rating 5). However, a key finding of this research is that the incorporation of biochar into an epoxy coating resulted in a significant improvement in hydrophobicity, which is measured using water contact angle. The incorporation of SW800 and SW1000 into coating formulations at varying concentrations resulted in an increase in water angle of approximately 18% and 20%, respectively. The outcomes of this project establish biochar-based coatings as a promising solution for eco-friendly and high-performance protective applications. Full article

Mulching is a sustainable agronomic practice that can improve soil quality and fruit characteristics in crops. This study investigated the influence of sheep wool mulch and a soil conditioner on growth, the accumulation of bioactive compounds, and soil enzymatic activity in apple orchards. A two-year field experiment (2023–2024) was conducted using three experimental methods: mulching with sheep wool (V2), application of a soil conditioner, corn starch-based polymer (V3), and a combination of sheep wool and corn starch-based polymer (V4) along with a control (V1). Tree growth parameters, fruit physicochemical properties, total phenolic and flavonoid content, and soil enzyme activities (dehydrogenase, catalase, phosphatase) were assessed. Data were analyzed using Principal Component Analysis (PCA) and Pearson’s correlation. PCA showed that the combined variant (V4) improved fruit size, weight, and bioactive compound content, while wool mulch alone (V2) was associated with higher fruit yield and better vegetative growth. Catalase activity correlated positively and consistently with bioactive compounds in both years, while phosphatase activity showed an intensified positive relationship in 2024. Dehydrogenase activity was negatively correlated with phenolic content in both seasons. Organic and integrated mulching practices can beneficially modulate both aboveground and belowground plant–soil interactions. The combined variant proved to be the most effective strategy, enhancing fruit nutritional quality and supporting sustainable apple orchard management. Full article