Search Results (58)

The peripartum period is critical for breeding female donkeys (i.e., jennies) and ensuring the delivery of healthy neonatal foals. The gut microbiota deeply influences the host metabolism. This study aimed to investigate the dynamic changes in the gut microbiome during the peripartum period in jennies. Fresh fecal samples of eight adult jennies were collected at the following seven sampling time points: 21, 7, and 3 days prepartum (G21, G7, and G3) and 1, 3, 7, and 14 days postpartum (L1, L3, L7, and L14). Sequencing of the V4 hypervariable regions of the 16S rRNA genes was carried out using fecal samples to identify the differences in the microbiome across the peripartum period. Bacteroidota and Firmicutes were the most abundant bacterial phyla in the feces. Treponema and Lachnospiraceae XPB1014 group significantly increased in the L3 group compared to the G7 group (q < 0.05), and a decline trend was observed in L1 group around parturition. The genus Clostridium sensu stricto 1, family Clostridiaceae, and order Clostridiales were considered to be biomarkers of the L3 group. Among the 25 functional pathways detected by Kyoto Encyclopedia of Genes and Genomes pathway analysis, beta lactam resistance, insulin resistance, and peptidases were the top three important pathways observed in the gut microbiota during the peripartum period in jennies. The gut microbial structure changed significantly at different time points during the peripartum period in jennies. These results contribute to a better understanding of the gut microbiota to ensure health care during important phases from late pregnancy to early lactation in jennies. Full article

The electrochemical CO₂ reduction reaction (eCO₂RR), driven by renewable energy, represents a promising strategy for mitigating atmospheric CO₂ levels while generating valuable fuels and chemicals. Its practical implementation hinges on the development of highly efficient electrocatalysts. In this study, a novel dual-metal atomic catalyst (DAC), composed of niobium and palladium single atoms anchored on a ferroelectric α-In₂Se₃ monolayer (Nb-Pd@In₂Se₃), is proposed based on density functional theory (DFT) calculations. The investigation encompassed analyses of structural and electronic characteristics, CO₂ adsorption configurations, transition-state energetics, and Gibbs free energy changes during the eCO₂RR process, elucidating a synergistic catalytic mechanism. The Nb-Pd@In₂Se₃ DAC system demonstrates enhanced CO₂ activation compared to single-atom counterparts, which is attributed to the complementary roles of Nb and Pd sites. Specifically, Nb atoms primarily drive carbon reduction, while neighboring Pd atoms facilitate oxygen species removal through proton-coupled electron transfer. This dual-site interaction lowers the overall reaction barrier, promoting efficient CO₂ conversion. Notably, the polarization switching of the In₂Se₃ substrate dynamically modulates energy barriers and reaction pathways, thereby influencing product selectivity. Our work provides theoretical guidance for designing ferroelectric-supported DACs for the eCO₂RR. Full article

The phase-sensitive optical time domain reflectometry (Φ-OTDR) has been widely applied in various fields. However, due to fading noise, false alarms often occur; this limits its engineering applications. In this paper, a fading suppression method for Φ-OTDR systems based on multi-domain multiplexing (MDM) is proposed. The principles and limitations of existing suppression methods such as spatial-domain multiplexing (SDM), polarization-domain multiplexing (PDM), and frequency-domain multiplexing (FDM) are analyzed. The principle of MDM is explained in detail, and an experimental system is established to test the fading noise suppression capabilities of different parameter combinations of the PDM, FDM, and SDM methods. Experimental results show that it is difficult to comprehensively suppress fading noise with single-domain multiplexing. Through optimizations of different parameter combinations, MDM can comprehensively suppress fading noise by appropriately selecting the number of FDM frequencies, the spatial weighting intervals, and using PDM, thus obtaining the optimal anti-fading solution between performance and hardware complexity. Through MDM, the fade-free measurement is achieved, providing a promising technical solution for the practical application of the Φ-OTDR technology. Full article

Background: Maize is easily contaminated by Aspergillus flavus, and the aflatoxin produced by A. flavus has been classified as a Group 1 carcinogen, for which there are currently no effective control measures. Biological control is regarded as an environmentally friendly and safe approach. Strains ZH179, ZH409, and ZH99 are three bacteria isolated from our laboratory that exhibit antagonistic effects against A. flavus. We conducted experiments to investigate their biocontrol efficacy. Results: The experimental results demonstrated that these three strains effectively inhibited A. flavus on plates and stored maize seeds. Identification revealed that ZH179 is Bacillus velezensis, while ZH409 and ZH99 are B. amyloliquefaciens. We also identified lipopeptide synthetase-related genes, including srfAA, srfAD, fenA, fenB, ituA, ituB, ituD, bmyA, bmyB, and bmyC, in these three strains. Furthermore, LC-MS analysis confirmed that these strains could produce lipopeptide compounds such as surfactin, fengycin, iturin, and bacillomycin. Using the Oxford cup method, we found that the lipopeptide compounds produced by these strains can inhibit the growth of A. flavus. Conclusion: These findings suggest that strains ZH179, ZH409, and ZH99 have good control effects on A. flavus during the storage of maize, primarily due to the lipopeptide compounds. This study provides a theoretical basis for using these three strains in the biological control of A. flavus. Full article

A double-cavity Fabry-Perot (F-P) interferometer sensor based on a polymer-filled hollow core fiber (HCF) has been proposed and experimentally verified. The double cavity of the sensor is formed by filling the hollow core fiber with two kinds of polymer materials and curing these materials, with the other end of the hollow core fiber connected to a single-mode fiber (SMF). The three reflective surfaces of the sensor reflect three beams of light, which interfere to form a spectrum with an envelope. By using Fast Fourier Transform (FFT) and a Fourier filter, the spectrum of each cavity can be separated and, based on this, the demodulation matrix of the sensor can be constructed. By controlling the length of the polymer cavity, a single sensor cavity can achieve high temperature and gas pressure sensitivity, with values of 2.05 nm/°C and 17.63 nm/MPa, respectively. More importantly, the sensor can be used under an environment of 40–110 °C and 0–3.0 MPa, with simple fabrication, good robustness, and better stability and repeatability compared to similar sensors. Based on its high sensitivity and large measurement range, this sensor has broad application prospects in industrial manufacturing and harsh environmental monitoring fields. Full article

Based on para-social interaction (PSI) theory and social identity perspective, this study explores the mechanisms driving consumers’ impulse buying in social live shopping. It examines how live content design, namely information comprehensiveness (INFCOM) and interactivity (INT), affects consumer cognition and affective experiences, namely perceived usefulness (PU), PSI, and sense of belonging (SOB), to generate the influence of the urge to buy impulsively (UBI), and further explores the moderating role of the consumer–broadcaster para-social relationship (PSR) between live content design and consumer experience. Findings indicate that in an accumulative social live shopping environment, comprehensive information and strong interactivity enhance consumer social identity, reduce shopping hesitations and obstacles, and encourage UBI. Forming a close consumer–broadcaster relationship is crucial for promoting social identity and increasing UBI. Even without interactive engagement, consumers who feel a close connection with the broadcaster still experience interaction and SOB. PSR influences impulse buying by enhancing consumer perceptions and thereby promoting UBI. This study advances the understanding of impulse buying from a social identity perspective and suggests that merchants and livestream designers can improve quality and sales by providing comprehensive product information and incorporating diverse interactive elements in live broadcasts. Full article

Potato common scab, caused by Streptomyces species, is a widespread soil-borne disease that poses a significant threat to potato cultivation globally. In this study, a Bacillus velezensis D7-8 strain was isolated from a potato. This endophytic bacterium exhibited broad-spectrum antifungal activity, and pot trials demonstrated that the D7-8 strain effectively controlled potato common scab with an efficacy of 42.07%. The complete genome sequence of the D7-8 strain was sequenced and subsequently identified as B. velezensis through multiple bioinformatic methods, primarily through structural variation analysis of whole-genome sequences. The machine learning method predicted that the expression profiles of colinear genes among closely related Bacillus species were highly consistent. Metabolite analysis of crude extracts using ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap high-resolution mass spectrometry (UPLC-Q-Exactive HRMS) revealed that D7-8 produces bioactive compounds, including surfactin and fengycin, both known for their antimicrobial properties. This study elucidates the antagonistic effect of B. velezensis D7-8 against Streptomyces acidiscabies and provides a valuable reference for future research on accurate microbial identification. Full article

Digital technologies represented by AR (Augmented Reality), VR (Virtual Reality), and digital twins, along with the expansion of metaverse platforms and digital marketing concepts, have attracted the attention of numerous sports fashion product consumers and brands, particularly in the category of sports shoes. Therefore, in the context of digital technologies, understanding the factors that affect consumer experience and the preferences in the online purchasing process of sports shoes is very important. This study employs Latent Dirichlet Allocation topic analysis to analyze 44,110 online user posts and comments from social platforms, extracting thematic elements of consumer experience needs for purchasing sports shoes online. The information obtained is further encoded and designed into a questionnaire, which is then utilized alongside the Kano model to analyze the overall preferences of consumer experience needs. The results indicate that webpage design and basic product information are considered as Must-be attributes for user experience needs; providing information on after-sales service policies and product comment, products’ special feature information, and online size testing are recognized as Performance attributes. Additionally, high-tech interaction methods, visual presentation, personalized customization, virtual try-on, apparel matching recommendations, and dressing scenario recommendations are identified as Attractive attributes. The study reveals that in the context of new digital technology development, the online shopping experience for sports shoes is enhanced across four dimensions: platform experience augmentation, product experience augmentation, user demand augmentation, and interactive experience augmentation. These four dimensions collectively constitute the holistic experience design for the online retail platform. Therefore, this research provides case references and theoretical insights for researchers and developers in the fields of brand marketing, experience design, and product service innovation. Full article

The quarantine pest, Cylas formicarius, is a key pest of sweet potatoes during both production and storage, posing a major threat to food security in various countries. To investigate behavioral mechanisms, the ultrastructure of the heads of larval and adult stages was analyzed using scanning electron microscopy, with an emphasis on the sensilla of the mouthparts and antennae. The results reveal degeneration of the antennae and ocelli in larvae. The larval mouthparts are equipped with three types and six subtypes of sensilla. Both male and female adults have four types and six subtypes of sensilla on their mouthparts. Compared to larvae, the adult mouthparts display a greater diversity of sensilla types and higher numbers of sensilla basicaonica (SB), sensilla chaetica (SC), and sensilla digitiformia (SD). Adult antennae consist of a scape, a pedicel, and eight flagellomeres (F1–F8), with F8 showing sexual dimorphism. Seven types of sensilla, excluding SB and sensilla ligulate (SL), each with two subtypes, were identified on the antennae of adults of both sexes. SC, sensilla furcatea, Böhm bristles, and SL were newly observed in the antennae of C. formicarius adults. Additionally, one type and seven subtypes of sensilla on the adult antennae exhibit distinct sexual dimorphism in terms of structure or number. The relationship between the head structure and adaptability of C. formicarius was examined, and the functions of each sensilla were discussed, providing a theoretical basis for future studies on the behavior of this pest. Full article

Background/Objectives: Dry eye (DE) is mainly characterized by dryness, foreign body sensation, eye pain and visual impairment. Their possible causes are mainly inflammation, tissue damage and neurosensory abnormalities, and vitamin B6 (VitB6) attenuates the inflammatory response by modulating the NF-κB pathway to quench reactive oxygen species (ROS). The aim of this experiment was to investigate the therapeutic effect of VitB6 eye drops on particulate matter 2.5 (PM2.5)-induced dry eye in mice. Methods: Mice induced with the dry eye group were first induced using PM2.5 eye drops in a standard environment for 14 days, and then treated with different concentrations of VitB6 eye drops for 14 consecutive days. The phenol red cotton test was used to measure tear production. Ocular inflammation index and tear film function were evaluated by slim microscopy. Hematoxylin–eosin (HE) staining was used to observe conjunctival and corneal structure. Periodate–Schiff (PAS) staining was used to quantify conjunctival goblet cells. Corneal cell apoptosis was determined by TUNEL assay. The expression of keratin 10 (K10) and p-NF-κB p65 was detected by immunofluorescent staining and Western blot analysis. Results: Mice using only the PM2.5 model all exhibited varying degrees of dry eye symptoms. VitB6 treatment increased tear secretion and reduced inflammatory indices in mice with increased nerve density and number of branches in the basement membrane of the corneal epithelium. Conclusions: We found that administering VitB6 eye drops has a therapeutic effect in PM2.5-induced DE. This observation suggests that VitB6 may be useful in the clinical therapy of DE. Full article

Currently, small object detection in complex remote sensing environments faces significant challenges. The detectors designed for this scenario have limitations, such as insufficient extraction of spatial local information, inflexible feature fusion, and limited global feature acquisition capability. In addition, there is a need to balance performance and complexity when improving the model. To address these issues, this paper proposes an efficient and lightweight SCM-YOLO detector improved from YOLOv5 with spatial local information enhancement, multi-scale feature adaptive fusion, and global sensing capabilities. The SCM-YOLO detector consists of three innovative and lightweight modules: the Space Interleaving in Depth (SPID) module, the Cross Block and Channel Reweight Concat (CBCC) module, and the Mixed Local Channel Attention Global Integration (MAGI) module. These three modules effectively improve the performance of the detector from three aspects: feature extraction, feature fusion, and feature perception. The ability of SCM-YOLO to detect small objects in complex remote sensing environments has been significantly improved while maintaining its lightweight characteristics. The effectiveness and lightweight characteristics of SCM-YOLO are verified through comparison experiments with AI-TOD and SIMD public remote sensing small object detection datasets. In addition, we validate the effectiveness of the three modules, SPID, CBCC, and MAGI, through ablation experiments. The comparison experiments on the AI-TOD dataset show that the mAP50 and mAP50-95 metrics of SCM-YOLO reach 64.053% and 27.283%, respectively, which are significantly better than other models with the same parameter size. Full article

Southern corn rust (SCR) is one of the most destructive foliar diseases in maize (Zea mays L.), resulting in significant yield losses. Therefore, the continuous identification of disease-resistant germplasm and the deployment of resistant hybrids is essential for durably controlling SCR. The objective of this research was to identify and characterize resistance loci against SCR in maize to expand disease management strategies. Here, we identified a maize landrace with high resistance to SCR ‘Silunuo’ (SLN) approaching complete immunity. We backcrossed it with a susceptible inbred line, N531, to generate a stable SCR-resistant introgression line N531_R. By crossing it with F35 (a susceptible inbred line), we created a large F₂ segregating population and mapped a major SCR-resistant locus on chromosome 10, known as RppSLN. Based on the genome assembly and annotation, we found that RppSLN harbors two NBS-LRR (nucleotide binding site–leucine-rich repeat) genes, namely Zmays10G000430 and Zmays10G000440. These NBS-LRR genes were significantly induced during artificial inoculation with Puccinia polysora, suggesting that they might be candidate genes collectively contributing to the resistance level at this locus. In conclusion, this study identified a major SCR resistance locus directly isolated from a landrace, providing valuable support and information for expanding new disease-resistant germplasms and promoting the utilization of landraces. Full article

Heat shock proteins (Hsps), acting as molecular chaperones, play a pivotal role in plant responses to environmental stress. In this study, we found a total of 192 genes encoding Hsps, which are distributed across all 12 chromosomes, with higher concentrations on chromosomes 1, 2, 3, and 5. These Hsps can be divided into six subfamilies (sHsp, Hsp40, Hsp60, Hsp70, Hsp90, and Hsp100) based on molecular weight and homology. Expression pattern data indicated that these Hsp genes can be categorized into three groups: generally high expression in almost all tissues, high tissue-specific expression, and low expression in all tissues. Further analysis of 15 representative genes found that the expression of 14 Hsp genes was upregulated by high temperatures. Subcellular localization analysis revealed seven proteins localized to the endoplasmic reticulum, while others localized to the mitochondria, chloroplasts, and nucleus. We successfully obtained the knockout mutants of above 15 Hsps by the CRISPR/Cas9 gene editing system. Under natural high-temperature conditions, the mutants of eight Hsps showed reduced yield mainly due to the seed setting rate or grain weight. Moreover, the rice quality of most of these mutants also changed, including increased grain chalkiness, decreased amylose content, and elevated total protein content, and the expressions of starch metabolism-related genes in the endosperm of these mutants were disturbed compared to the wild type under natural high-temperature conditions. In conclusion, our study provided new insights into the HSP gene family and found that it plays an important role in the formation of rice quality and yield. Full article

Immunosuppression undermines intestinal barrier integrity. Cow placenta extract (CPE) primarily consists of active peptides with immunomodulatory and antioxidant effects. This study aimed to examine the preventive effect of CPE against intestinal damage induced by cyclophosphamide (Cy) in immunosuppressed mice. Thirty-six mice were randomly allocated into three groups: control group (C), model group (M), and treatment group (CPE). The mice in the CPE group were provided with 1500 mg/kg/day of CPE via gavage. In the last 3 days, mice in the groups M and CPE received intraperitoneal injections of 80 mg/kg/day of Cy. The results showed that CPE improved intestinal barrier function by decreasing serum d-Lactate (D-LA) levels and diamine oxidase (DAO) activity, while elevating the relative expression of Occludin, zonula occludens-1 (ZO-1), and mucin-2 (MUC-2) mRNA. Additionally, CPE improved the immune organ index and elevated the levels of secretory immunoglobulin A (sIgA), superoxide dismutase (SOD), interleukin-1beta (IL-1β), interleukin-4 (IL-4), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF-α) in the intestine, thereby enhancing intestinal mucosal immune function. Furthermore, CPE improved the diversity of intestinal microbiota and increased the abundance of Candidatus_Saccharimonas, Psychrobacter, and Enterorhabdus, which promoted the proper functioning of the intestines. These findings suggest that CPE effectively ameliorates Cy-induced intestinal damage by enhancing the intestinal barrier, improving immune function, and restoring intestinal microbiota. Full article

To further develop Liupao tea products and enhance their flavor, this study investigated the effects of different fermentation methods on the aroma quality of Liupao tea. The aroma quality of Liupao tea was comprehensively analyzed using HS-SPME in combination with GC-Q-TOF-MS, electronic nose, and sensory evaluations. Electronic nose detection showed that the aroma fingerprints of Liupao tea samples with different fermentation methods were different. Sulfides, alcohols, ketones, and methyls were the main aroma categories affecting the aroma of the four groups of Liupao tea samples. GC-Q-TOF-MS analysis revealed significant differences in the composition of aroma components among the four fermentation methods of Liupao tea (p < 0.05). Furthermore, the total amount of aroma compounds was found to be highest in the group subjected to hot fermentation combined with the inoculation of Monascus purpureus (DMl group). Based on the OPLS-DA model, candidate differential aroma components with VIP > 1 were identified, and characteristic aroma compounds were selected based on OAV > 10. The key characteristic aroma compounds shared by the four groups of samples were 1,2,3-Trimethoxybenzene with a stale aroma and nonanal with floral and fruity aromas. The best sensory evaluation results were obtained for the DMl group, and its key characteristic aroma compounds mainly included 1,2,3-Trimethoxybenzene, nonanal, and cedrol. The results of this study can guide the development of Liupao tea products and process optimization. Full article