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Authors = Yan-Lin Shao

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11 pages, 2151 KiB  
Article
Fabrication of Antibacterial Poly(ethylene terephthalate)/Graphene Nanocomposite Fibers by In Situ Polymerization for Fruit Preservation
by Jiarui Wu, Qinhan Chen, Aobin Han, Min Liu, Wenhuan Zhong, Xiaojue Shao, Yan Jiang, Jing Lin, Zhenyang Luo, Jie Yang and Gefei Li
Molecules 2025, 30(15), 3109; https://doi.org/10.3390/molecules30153109 - 24 Jul 2025
Viewed by 231
Abstract
A novel polyester/graphene nanocomposite fiber was produced using the in situ polymerization protocol with carboxylated graphene and melt spinning technology. The resulting nanocomposite fibers were characterized by X-ray diffraction (XRD), Raman spectroscopy, differential scanning calorimeter (DSC), and scanning electron microscope (SEM). The fibers [...] Read more.
A novel polyester/graphene nanocomposite fiber was produced using the in situ polymerization protocol with carboxylated graphene and melt spinning technology. The resulting nanocomposite fibers were characterized by X-ray diffraction (XRD), Raman spectroscopy, differential scanning calorimeter (DSC), and scanning electron microscope (SEM). The fibers containing 0.2 wt% graphene fraction showed an excellent dispersity of graphene nanosheets in polymeric matrix. DSC test showed that the efficient polymer-chain grafting depresses the crystallization of PET chains. This graphene-contained PET fabric exhibited attractive antibacterial properties that can be employed in fruit preservation to ensure food safety. Full article
(This article belongs to the Special Issue Design and Application of Functional Supramolecular Materials)
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15 pages, 6104 KiB  
Article
Development of a Noninfectious Japanese Encephalitis Virus Replicon for Antiviral Drug Screening and Gene Function Studies
by Yang Yang, Jiayang Zheng, Yafang Lin, Yan Zhang, Qianming Zhao, Hailong Zhang, Junjie Zhang, Zongjie Li, Ke Liu, Beibei Li, Donghua Shao, Yafeng Qiu, Zhiyong Ma and Jianchao Wei
Viruses 2025, 17(6), 759; https://doi.org/10.3390/v17060759 - 27 May 2025
Viewed by 561
Abstract
Viral replicons are efficient tools to understand the mechanisms of viral replication and screen antiviral drugs. In this study, a viral-cDNA-based replicon of Japanese encephalitis virus (JEV), which is the causative agent of Japanese encephalitis, was constructed by replacing the viral structural proteins [...] Read more.
Viral replicons are efficient tools to understand the mechanisms of viral replication and screen antiviral drugs. In this study, a viral-cDNA-based replicon of Japanese encephalitis virus (JEV), which is the causative agent of Japanese encephalitis, was constructed by replacing the viral structural proteins with a green fluorescent protein (JEV-GFP replicon). The resulting JEV-GFP replicon was used as a tool to screen antiviral drugs targeting JEV nonstructural proteins, and the five compounds JNJ-A07, HZ-1157, NITD-2, quinine, and NITD008 were obtained, which significantly inhibited the replication of the JEV-GFP replicon and JEV in vitro, and the properties of these five compounds were also analyzed. The CC50, EC50, and SI indices of these five compounds were analyzed. In addition, the JEV-GFP replicon was used as a tool to identify the residues of viral nonstructural proteins involved in RNA replication, and the cysteine residue at position 4 of nonstructural protein 1 was found to be essential for JEV RNA replication. These data suggested that the noninfectious JEV-GFP replicon could be used as tool for different purposes, such as antiviral drug screening and gene function studies. Full article
(This article belongs to the Section Animal Viruses)
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37 pages, 7855 KiB  
Article
Disturbance Sensitivity of Proximity Construction in Subway Protection Zone
by Zhi-Qun Gong, Yong-Zhi Wang, Wei-Ke Zhou, Shao-Ming Liao, Yan-Qing Men and Song-Chao Lin
CivilEng 2025, 6(2), 28; https://doi.org/10.3390/civileng6020028 - 21 May 2025
Viewed by 1295
Abstract
The analysis of the impact of the construction of the subway protection zone on the adjacent subway tunnel has become the premise on which to ensure the safe operation of the tunnel. The need for expert members to carry out safety assessments based [...] Read more.
The analysis of the impact of the construction of the subway protection zone on the adjacent subway tunnel has become the premise on which to ensure the safe operation of the tunnel. The need for expert members to carry out safety assessments based on specific calculations to determine the impact of construction on the safety of protected tunnels is extremely inconvenient for safety management and significantly reduces management efficiency. This paper analyzes and qualitatively judges the influence range and disturbance size of pile foundation construction, shallow foundation engineering, and foundation pit excavation. Based on relevant research results from scholars and numerical simulation methods, quantitative analysis and comparison are performed on the parameter sensitivity of pile foundation engineering, shallow foundation engineering, and foundation pit engineering along the subway line, and the influence of multi-factor combination is studied and discussed to obtain the influence sensitivity of each factor. The results show that the increase in pile spacing can effectively reduce the pile group effect. The sensitivity of subway tunnel settlement displacement is mainly controlled by the settlement displacement value. The larger the settlement displacement is, the stronger the sensitivity is. The loaded pile foundation arranged along the direction of the subway tunnel has more obvious disturbance to the subway tunnel than that arranged perpendicular to the direction of the subway tunnel. Full article
(This article belongs to the Section Construction and Material Engineering)
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20 pages, 37692 KiB  
Article
Environmentally Sustainable Lithium Exploration: A Multi-Source Remote Sensing and Comprehensive Analysis Approach for Clay-Type Deposits in Central Yunnan, China
by Yan Li, Xiping Yuan, Shu Gan, Changsi Mu, Zhi Lin, Xiong Duan, Yanyan Shao, Yanying Wang and Lin Hu
Sustainability 2025, 17(8), 3732; https://doi.org/10.3390/su17083732 - 21 Apr 2025
Viewed by 652
Abstract
Carbonate-hosted clay-type lithium deposits have emerged as strategic resources critical to the global energy transition, yet their exploration faces the dual challenges of technical complexity and environmental sustainability. Traditional methods often entail extensive land disruption, particularly in ecologically sensitive ecosystems where vegetation coverage [...] Read more.
Carbonate-hosted clay-type lithium deposits have emerged as strategic resources critical to the global energy transition, yet their exploration faces the dual challenges of technical complexity and environmental sustainability. Traditional methods often entail extensive land disruption, particularly in ecologically sensitive ecosystems where vegetation coverage and weathered layers hinder mineral detection. This study presents a case study of the San Dan lithium deposit in central Yunnan, where we propose a hierarchical anomaly extraction and multidimensional weighted comprehensive analysis. This comprehensive method integrates multi-source data from GF-3 QPSI SAR, GF-5B hyperspectral, and Landsat-8 OLI datasets and is structured around two core parts, as follows: (1) Hierarchical Anomaly Extraction: Utilizing principal component analysis, this part extracts hydroxyl and iron-stained alteration anomalies. It further employs the spectral hourglass technique for the precise identification of lithium-rich minerals, such as montmorillonite and illite. Additionally, concealed structures are extracted using azimuth filtering and structural detection in radar remote sensing. (2) Multidimensional Weighted Comprehensive Analysis: This module applies reclassification, kernel density analysis, and normalization preprocessing to five informational layers—hydroxyl, iron staining, minerals, lithology, and structure. Dynamic weighting, informed by expert experience and experimental adjustments using the weighted weight-of-evidence method, delineates graded target areas. Three priority target areas were identified, with field validation conducted in the most promising area revealing Li2O contents ranging from 0.10% to 0.22%. This technical system, through the collaborative interpretation of multi-source data and quantitative decision-making processes, provides robust support for exploring carbonate-clay-type lithium deposits in central Yunnan. By promoting efficient, data-driven exploration and minimizing environmental disruption, it ensures that lithium extraction meets the growing demand while preserving ecological integrity, setting a benchmark for the sustainable exploration of clay-type lithium deposits worldwide. Full article
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18 pages, 4052 KiB  
Article
Sequential Discrimination of Mixed Quantum States
by Jin-Hua Zhang, Fu-Lin Zhang, Yan Gao, Wei Qin and Shao-Ming Fei
Entropy 2025, 27(3), 246; https://doi.org/10.3390/e27030246 - 27 Feb 2025
Viewed by 569
Abstract
Classical mixtures of quantum states often give rise to decoherence and are generally considered detrimental to quantum processing. However, in the framework of sequential measurement, such mixtures can be beneficial for state discrimination. We investigate the sequential discrimination of mixed states and compare [...] Read more.
Classical mixtures of quantum states often give rise to decoherence and are generally considered detrimental to quantum processing. However, in the framework of sequential measurement, such mixtures can be beneficial for state discrimination. We investigate the sequential discrimination of mixed states and compare the results with those of pure states under the condition of equal fidelity. It is found that the successful probability of the mixed-state protocol is superior to the pure one under the equal-fidelity condition. It is shown that the difference between the sequential discrimination of pure and mixed states is more reliable under the equal-fidelity condition than under single-shot discrimination, and this difference increases with the mixability of the initial mixed states. For scenarios in which classical communication is allowed, the optimal successful probability of pure-state discriminations is larger than that for mixed states on the contrary. We also show that the classical mixture of basic vectors from quantum decoherence has a subtle impact on the communication channel induced by the coincidence of the maximal mutual information and optimal successful probability of sequential discrimination for pure states. Full article
(This article belongs to the Section Quantum Information)
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21 pages, 5814 KiB  
Article
Melatonin Enhances Maize Germination, Growth, and Salt Tolerance by Regulating Reactive Oxygen Species Accumulation and Antioxidant Systems
by Wei-Qing Li, Jia-Yu Li, Shao-Jie Bi, Jia-Yue Jin, Zhong-Ling Fan, Zi-Lin Shang, Yi-Fei Zhang and Yan-Jie Wang
Plants 2025, 14(2), 296; https://doi.org/10.3390/plants14020296 - 20 Jan 2025
Cited by 4 | Viewed by 1290
Abstract
Melatonin (MT) is a crucial hormone that controls and positively regulates plant growth under abiotic stress, but the biochemical and physiological processes of the combination of melatonin seed initiation and exogenous spray treatments and their effects on maize germination and seedling salt tolerance [...] Read more.
Melatonin (MT) is a crucial hormone that controls and positively regulates plant growth under abiotic stress, but the biochemical and physiological processes of the combination of melatonin seed initiation and exogenous spray treatments and their effects on maize germination and seedling salt tolerance are not well understood. Consequently, in this research, we utilized the maize cultivars Zhengdan 958 (ZD958) and Demeiya 1 (DMY1), which are extensively marketed in northeastern China’s high-latitude cold regions, to reveal the modulating effects of melatonin on maize salinity tolerance by determining the impacts of varying concentrations of melatonin on maize seedling growth characteristics, osmoregulation, antioxidant systems, and gene expression. The findings revealed that salt stress (100 mM NaCl) significantly inhibited maize seed germination and seedling development, which resulted in significant increases in the H2O2 and O2 content and decreases in the antioxidant enzyme activity and photosynthetic pigment content in maize seedlings. However, exogenous melatonin considerably reduced the development inhibition caused by salt stress in maize seedlings. Moreover, exogenous melatonin alleviated NaCl-induced membrane damage and oxidative stress, and reduced Na+ content and excessively large quantities of reactive oxygen species (ROS). In addition, exogenous melatonin increased antioxidant enzyme activity and the expression of the antioxidant enzyme genes ZmSOD4, ZmCAT2, and ZmAPX2. This study demonstrates the potential role of combined melatonin seed initiation and foliar spray treatments in mitigating the detrimental effects of salt stress on maize growth, giving a theoretical foundation to future research on the possible advantages of exogenous regulating chemicals in attaining sustainable production in salt-alkaline soils. Full article
(This article belongs to the Section Crop Physiology and Crop Production)
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13 pages, 4333 KiB  
Article
Design and Synthesis of Phthalocyanine-Sensitized Titanium Dioxide Photocatalysts: A Dual-Pathway Study
by Qi Shao, Jiaqi Liu, Qiwang Chen, Jing Yu, Zhongbao Luo, Rongqiang Guan, Zichen Lin, Mingxuan Li, Yi Li, Cong Liu and Yan Li
Materials 2025, 18(1), 202; https://doi.org/10.3390/ma18010202 - 5 Jan 2025
Viewed by 1252
Abstract
Phthalocyanine-sensitized TiO2 significantly enhances photocatalytic performance, but the method of phthalocyanine immobilization also plays a crucial role in its performance. In order to investigate the effect of the binding strategy of phthalocyanine and TiO2 on photocatalytic performance, a dual-pathway study has [...] Read more.
Phthalocyanine-sensitized TiO2 significantly enhances photocatalytic performance, but the method of phthalocyanine immobilization also plays a crucial role in its performance. In order to investigate the effect of the binding strategy of phthalocyanine and TiO2 on photocatalytic performance, a dual-pathway study has been conducted. On the one hand, zinc-tetra (N-carbonylacrylic) aminephthalocyanine (Pc) was directly grafted onto the surface of Fe3O4@SiO2@TiO2 (FST). On the other hand, Pc was immobilized on a silane coupling agent ((3-aminopropyl) triethoxysilane) grafted onto the surface of the FST. Through photocatalytic experiments on the two types of composite materials synthesized, the results showed that the photocatalyst obtained by directly sensitizing Pc (FSTP) exhibited better performance on rhodamine B(RhB) removal than did the other photocatalyst using the silane coupling agent (FSTAP). Further mechanistic studies showed that directly sensitized FSTP exhibited more efficient photogenerated electron–hole pair separation, whereas FSTAP linked by a silane coupling agent created an additional transport distance that might greatly affect the photogenerated electron transport. Therefore, the dual-pathway research in this work provides new guidance for efficiently constructing phthalocyanine-sensitized TiO2 photocatalysts. Full article
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16 pages, 7560 KiB  
Article
Zirconium-Modified Attapulgite Composite for Phosphorus Removal and Algae Control in Lake Water
by Chun-Yan Lin, Wenliang Wu and Wenya Shao
Water 2024, 16(22), 3233; https://doi.org/10.3390/w16223233 - 10 Nov 2024
Cited by 1 | Viewed by 1366
Abstract
Phosphorus removal is critical for effective water treatment and the prevention of eutrophication. This study focuses on the modification of attapulgite, an economical clay material, with zirconium (Zr@ATP) to enhance its phosphorus adsorption capacity. Zr@ATP was comprehensively characterized, and its phosphorus-removal mechanisms were [...] Read more.
Phosphorus removal is critical for effective water treatment and the prevention of eutrophication. This study focuses on the modification of attapulgite, an economical clay material, with zirconium (Zr@ATP) to enhance its phosphorus adsorption capacity. Zr@ATP was comprehensively characterized, and its phosphorus-removal mechanisms were investigated. Additionally, its performance in water treatment was evaluated using a lake water-sediment system. Zr@ATP exhibited a high surface area of 329.29 m2/g. The static adsorption experiments revealed that Zr@ATP achieved a phosphorus-removal efficiency of 95.8% at an adsorbent dosage of 5 g/L. Kinetic studies indicated that the adsorption followed a pseudo-second-order model, with the primary mechanism being chemisorption via ion exchange. Application of Zr@ATP in a lake water-sediment system resulted in an 83.6% reduction in total phosphorus. The chlorophyll concentration significantly decreased from 32.33 μg/L to 8.56 μg/L, and the algal density decreased by 84.6%, effectively inhibiting algal growth. These results suggest that Zr@ATP is a promising adsorbent for sustainable phosphorus removal and eutrophication control in aquatic environments. Full article
(This article belongs to the Section Urban Water Management)
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36 pages, 8134 KiB  
Article
A Study on the Influence Mechanisms of Neighborhood Vitality and the Characteristics of Spatial and Temporal Differentiation in the Urban Fringe Areas of Wuhan City
by Yan Long, Siyu Hu, Zhengyuan Lu, Lianghao Cheng, Cairui Zhen, Jingmei Shao, Yuqiao Zheng, Xuehui Zhou, Jin Li, Yudi Lin, Shu Zhang, Yue Wang, Tianyue Luo, Haijuan Zhao and Xuejun Liu
Land 2024, 13(11), 1771; https://doi.org/10.3390/land13111771 - 28 Oct 2024
Cited by 1 | Viewed by 1360
Abstract
Achieving effective integration of urban–rural relationships and promoting the flow of resources between urban and rural areas in megacities are a key priority in the development of China’s new urbanization efforts. As a transitional zone between urban and rural areas, the urban fringe [...] Read more.
Achieving effective integration of urban–rural relationships and promoting the flow of resources between urban and rural areas in megacities are a key priority in the development of China’s new urbanization efforts. As a transitional zone between urban and rural areas, the urban fringe is the frontier of urban–rural integration. The specific research object of this paper is the urban fringe areas of Wuhan City. This paper quantifies the neighborhood vitality of the fringe areas by the short-stay visitors in the fringe areas and selects the 5D elements of the built environment and social media data from multiple sources to construct the indicator system assessing the neighborhood vitality of the urban fringe areas. This paper analyzes the spatial distribution characteristics of neighborhood vitality and its influencing factors in urban fringe areas and investigates the connection between neighborhood vitality and its influencing factors through the application of the multi-scale geographically weighted regression (MGWR) model. Based on the regression results, relevant planning recommendations are made on how to enhance the vitality of neighborhoods in urban fringe areas. The results show that the index system constructed by “5D” elements of built environment and social media data can well explain the spatial distribution of neighborhood vitality in urban fringe areas. Among the influencing factors, the absolute value of the correlation coefficient of network exposure is the largest, followed by road density and functional density. Thanks to the different bandwidths given by MGWR to the influencing factors, the global influencing factors are only two indicators—development intensity and functional mixing degree—while the other influencing factors are all local, and the influence degree of different regions is different, so it is necessary to analyze and put forward different planning suggestions accordingly. Full article
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29 pages, 31900 KiB  
Review
Multiscale Models of CVD Process: Review and Prospective
by Yu Tian, Zefan Yan, Lin Jiang, Rongzheng Liu, Bing Liu, Youlin Shao, Xu Yang and Malin Liu
Materials 2024, 17(20), 5131; https://doi.org/10.3390/ma17205131 - 21 Oct 2024
Cited by 6 | Viewed by 3739
Abstract
Chemical vapor deposition (CVD) is a crucial technique in the preparation of high-quality thin films and coatings, and is widely used in various industries including semiconductor, optics, and nuclear fuel, due to its operation simplicity and high growth rate. The complexity of the [...] Read more.
Chemical vapor deposition (CVD) is a crucial technique in the preparation of high-quality thin films and coatings, and is widely used in various industries including semiconductor, optics, and nuclear fuel, due to its operation simplicity and high growth rate. The complexity of the CVD process arises from numerous parameters, such as precursor chemistry, temperature, pressure, gas flow dynamics, and substrate characteristics. These multiscale parameters make the optimization of the CVD process a challenging task. Numerical simulations are widely used to model and analyze the CVD complex systems, and can be divided into nanoscale, mesoscale, and macroscale methods. Numerical simulation is aimed at optimizing the CVD process, but the inter-scale parameters still need to be extracted in modeling processes. However, multiscale coupling modeling becomes a powerful method to solve these challenges by providing a comprehensive framework that integrates phenomena occurring at different scales. This review presents an overview of the CVD process, the common critical parameters, and an in-depth analysis of CVD models in different scales. Then various multiscale models are discussed. This review highlights the models in different scales, integrates these models into multiscale frameworks, discusses typical multiscale coupling CVD models applied in practice, and summarizes the parameters that can transfer information between different scales. Finally, the schemes of multiscale coupling are given as a prospective view. By offering a comprehensive view of the current state of multiscale CVD models, this review aims to bridge the gap between theory and practice, and provide insights that could lead to a more efficient and precise control of the CVD process. Full article
(This article belongs to the Section Materials Simulation and Design)
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14 pages, 2631 KiB  
Article
A Method for Evaluating Demand Response Potential of Industrial Loads Based on Fuzzy Control
by Yan Li, Zhiwen Liu, Chong Shao, Bingjun Lin, Jiayu Rong, Nan Dong, Buyun Su and Yuejia Hong
Energies 2024, 17(20), 5146; https://doi.org/10.3390/en17205146 - 16 Oct 2024
Viewed by 936
Abstract
Demand response (DR) can ensure electricity supply security by shifting or shedding loads, which plays an important role in a power system with a high proportion of renewable energy sources. Industrial loads are vital participants in DR, but it is difficult to assess [...] Read more.
Demand response (DR) can ensure electricity supply security by shifting or shedding loads, which plays an important role in a power system with a high proportion of renewable energy sources. Industrial loads are vital participants in DR, but it is difficult to assess DR potential because of many complex factors. In this paper, a new method based on fuzzy control is given to assess the DR potential of industrial loads. A complete assessment framework including four steps is presented. Firstly, the industrial load data are preprocessed to mitigate the influence of noisy and transmission losses, and then the K-means algorithm considering the optimal cluster number is used to calculate baseline load of industrial load. Subsequently, an open-loop fuzzy controller is designed to predict the response factor of different industrial loads. Three strongly correlated indicators, namely peak load rate, electricity intensity, and load flexibility, are selected as the input of fuzzy control, which represents response willingness. Finally, the baseline load of diverse clustering scenarios and the response factor are used to calculate the DR potential of different industrial loads. The proposed method takes into account both economic and technical factors comprehensively, and thus, the results better represent the available DR potential in real-world situations. To demonstrate the effectiveness of the proposed method, the case of a medium-sized city in China is studied. The simulation focuses on the top eight industrial types, and the results show they can contribute about 189 MW available DR potential. Full article
(This article belongs to the Section K: State-of-the-Art Energy Related Technologies)
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9 pages, 3046 KiB  
Article
Molybdenum Truncated Cone Arrays with Localized Surface Plasmon Resonance for Surface-Enhanced Raman Scattering Application
by Cheng Wang, Tao Cui, Zhe Liu, Yu Lin, Shuai Tang, Lei Shao, Huanjun Chen, Yan Shen and Shaozhi Deng
Photonics 2024, 11(10), 950; https://doi.org/10.3390/photonics11100950 - 10 Oct 2024
Cited by 1 | Viewed by 1266
Abstract
Plasmonic materials have been extensively explored for surface-enhanced Raman scattering (SERS) due to their high tunability and excellent localized electric field enhancement. Most research for now has focused on noble metals, with limited investigation into corrosion-resistant materials for SERS effects. In this study, [...] Read more.
Plasmonic materials have been extensively explored for surface-enhanced Raman scattering (SERS) due to their high tunability and excellent localized electric field enhancement. Most research for now has focused on noble metals, with limited investigation into corrosion-resistant materials for SERS effects. In this study, a photolithography process is firstly used to create a patterned dot array on a silicon substrate. Next, magnetron sputtering is employed to deposit molybdenum films, finally resulting in the molybdenum truncated cone array substrates for SERS applications. The fabricated truncated cone array experimentally facilitates the coupling of localized surface plasmon polaritons, consistent with simulation results obtained via the finite-difference time-domain method. The formation of hot spots between the cone unit cell arrays leads to the improved Raman signals and can act as traps for target molecules. This study demonstrates that molybdenum-based micro-nano structures can serve as reliable SERS substrates for sensitive molecular sensing applications in highly corrosive environments. Full article
(This article belongs to the Special Issue Micro-Nano Optical Devices)
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16 pages, 5704 KiB  
Article
Design and Manufacture of 30-Degree Projection Lens for Augmented Reality Waveguide
by Wen-Shing Sun, Ying-Shun Hsu, Chuen-Lin Tien, Wen-Kai Lin, Yi-Lun Su, Jun-Yi Yu, Shao-Kui Zhou, Yuan-Yan Liang, Wan-Pin Tsai, Chi Sun, Tsung-Xian Lee, Wei-Chia Su, Shiuan-Huei Lin and Ching-Cherng Sun
Micromachines 2024, 15(10), 1198; https://doi.org/10.3390/mi15101198 - 27 Sep 2024
Cited by 4 | Viewed by 2550
Abstract
A projection lens with a 30-degree field of view is developed for use in augmented reality (AR) glasses, including a waveguide combiner designed for a 0.35-inch LCoS panel. The entrance pupil diameter of the lens is 14 mm and the lens has an [...] Read more.
A projection lens with a 30-degree field of view is developed for use in augmented reality (AR) glasses, including a waveguide combiner designed for a 0.35-inch LCoS panel. The entrance pupil diameter of the lens is 14 mm and the lens has an effective focal length of 16.443 mm; an F-number of 1.175. This paper has four key issues: optical projection lens design, lens manufacturing and assembly tolerance analysis, projection lens resolution testing, and AR glasses system resolution testing of panel images projected by the projection lens. After lens manufacture, the lens was tested, achieving a central field image quality of 57 cycles/mm, an angular resolution of 33 pixels per degree (PPD), a 0.7 field image quality of 40.3 cycles/mm, and an angular resolution of 23 pixels per degree (PPD). Imaging performance testing based on a diffraction-type waveguide shows a resolution of 57 cycles/mm in the center area and an angular resolution of 33 PPD. Full article
(This article belongs to the Special Issue Photonic and Optoelectronic Devices and Systems, Second Edition)
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14 pages, 1368 KiB  
Article
CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole (Cynoglossus semilaevis) Testis Cells
by Yanxu Sun, Hong-Yan Wang, Binghua Liu, Bowen Yue, Qian Liu, Yuyan Liu, Ivana F. Rosa, Lucas B. Doretto, Shenglei Han, Lei Lin, Xiaoling Gong and Changwei Shao
Int. J. Mol. Sci. 2024, 25(14), 7637; https://doi.org/10.3390/ijms25147637 - 11 Jul 2024
Viewed by 1960
Abstract
DNA methylation is a key epigenetic mechanism orchestrating gene expression networks in many biological processes. Nonetheless, studying the role of specific gene methylation events in fish faces challenges. In this study, we validate the regulation of DNA methylation on empty spiracles homeobox 2 [...] Read more.
DNA methylation is a key epigenetic mechanism orchestrating gene expression networks in many biological processes. Nonetheless, studying the role of specific gene methylation events in fish faces challenges. In this study, we validate the regulation of DNA methylation on empty spiracles homeobox 2 (emx2) expression with decitabine treatment in Chinese tongue sole testis cells. We used the emx2 gene as the target gene and developed a new DNA methylation editing system by fusing dnmt3a with catalytic dead Cas9 (dCas9) and demonstrated its ability for sequence-specific DNA methylation editing. Results revealed that utilizing dCas9-dnmt3a to target emx2 promoter region led to increased DNA methylation levels and decreased emx2 expression in Chinese tongue sole testis cells. More importantly, the DNA methylation editing significantly suppressed the expression of MYC proto-oncogene, bHLH transcription factor (myc), one target gene of emx2. Furthermore, we assessed the off-target effects of dCas9-dnmt3a and confirmed no significant impact on the predicted off-target gene expression. Taken together, we developed the first DNA methylation editing system in marine species and demonstrated its effective editing ability in Chinese tongue sole cells. This provides a new strategy for both epigenetic research and molecular breeding of marine species. Full article
(This article belongs to the Section Molecular Biology)
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14 pages, 2049 KiB  
Article
Effects of Cascara Cherry and Other Coffee Litter Mulching on Soil Properties, Photosynthesis, and Water Use Efficiency of Coffea Canephora Pierre ex A. Froehner cv. Reyan No.1 Seedling
by Ang Zhang, Su-Sen Chen, Xing-Jun Lin, Lin Yan, Yan-Li Huang, Yan Sun, Qing-Yun Zhao, Shao-Guan Zhao, Li-Hua Li, Yu-Zhou Long and Yun-Ping Dong
Agronomy 2024, 14(7), 1418; https://doi.org/10.3390/agronomy14071418 - 29 Jun 2024
Viewed by 1318
Abstract
Mulching cultivation with agricultural wastes is the main production pattern of coffee at present, but the effect of mulching cultivation on photosynthetic physiological processes of coffee plants is still not clear. Therefore, a randomized block design was adopted to establish a field experiment [...] Read more.
Mulching cultivation with agricultural wastes is the main production pattern of coffee at present, but the effect of mulching cultivation on photosynthetic physiological processes of coffee plants is still not clear. Therefore, a randomized block design was adopted to establish a field experiment by one-year-old Coffee Canephora seedlings in this study. There were four types of mulch treatments, including non-mulch coffee waste (C), mulching coffee litter (L), mulching coffee cascara (cherry pericarp, P), and mulching coffee litter and cascara (LP) in this field experiment. Soil properties and microenvironment (e.g., moisture, temperature, pH, bulk density, organic matter content, alkali-hydrolyzed nitrogen content, available potassium content, and available potassium content), agronomic traits (e.g., specific leaf area, leaf area index, plant height, and relative chlorophyll content), and photosynthetic indices (e.g., photosynthesis, transpiration, respiration, stomatal conductance, intercellular CO2 concentration, water use efficiency, and carbon use efficiency) were investigated to determine the effects of different coffee waste mulches on the photosynthetic physiology of coffee seedlings. The results show that coffee litter and cascara mulch significantly reduced soil temperature by 0.42 or 0.33 °C, respectively, and coffee litter rather than cascara mulch significantly increased the soil’s available potassium content by 46.28%, although coffee waste mulch did not affect other soil properties or microenvironment indices; coffee cascara mulching significantly increased the specific leaf area and net and gross photosynthesis of coffee by 45.46%, 78.33%, and 91.72%, respectively, but the mulching treatments did not affect stomatal conductance, transpiration, or carbon use efficiency in this study. Additionally, coffee cascara mulching increased leaf respiration and net and gross water use efficiency by 109.34%, 80.54%, and 104.95%, respectively. The coffee cascara mulching alone had the most significant positive impact on the photosynthetic index, followed by a combination of litter and cascara, litter alone, and the control treatment. The observed variations in the coffee photosynthetic index may be attributed to the reduction of soil temperature caused by mulching treatments rather than the increase in soil nutrients content. These results indicate that coffee cascara mulching could effectively promote photosynthesis and the growth of coffee seedlings by improving the soil microenvironment. Full article
(This article belongs to the Section Horticultural and Floricultural Crops)
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