Different Forms of TFF3 in the Human Saliva: Heterodimerization with IgG Fc Binding Protein (FCGBP)
Abstract
:1. Introduction
2. Results
2.1. Characterization of TFF3 in Human Saliva by SEC and Western Blot Analysis
2.2. Purification of the Low-Molecular-Mass Form of TFF3 and Characterization by Mass Spectrometric Proteomics
3. Discussion
3.1. Salivary TFF3 Forms High-Molecular-Mass Heterodimers with FCGBP
3.2. TFF3 Homodimers and Degradation in Human Saliva
4. Materials and Methods
4.1. Human Saliva
4.2. Protein Purification by FPLC
4.3. SDS-PAGE, Agarose Gel Electrophoresis, and Western Blot Analysis
4.4. Mass Spectrometric Proteomics of in-Gel Digested Proteins, Database Searching
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AgGE | Agarose gel electrophoresis |
DMBT | Deleted in Malignant Brain Tumor |
FCGBP | IgG Fc binding protein |
FPLC | Fast protein liquid chromatography |
LC-ESI-MS/MS | Liquid chromatography-electrospray ionization-tandem mass spectrometry |
PAS | Periodic acid-Schiff |
SDS-PAGE | Sodium dodecyl sulfate polyacrylamide gel electrophoresis |
SEC | Size exclusion chromatography |
TFF | Trefoil factor family |
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Houben, T.; Harder, S.; Schlüter, H.; Kalbacher, H.; Hoffmann, W. Different Forms of TFF3 in the Human Saliva: Heterodimerization with IgG Fc Binding Protein (FCGBP). Int. J. Mol. Sci. 2019, 20, 5000. https://doi.org/10.3390/ijms20205000
Houben T, Harder S, Schlüter H, Kalbacher H, Hoffmann W. Different Forms of TFF3 in the Human Saliva: Heterodimerization with IgG Fc Binding Protein (FCGBP). International Journal of Molecular Sciences. 2019; 20(20):5000. https://doi.org/10.3390/ijms20205000
Chicago/Turabian StyleHouben, Till, Sönke Harder, Harmut Schlüter, Hubert Kalbacher, and Werner Hoffmann. 2019. "Different Forms of TFF3 in the Human Saliva: Heterodimerization with IgG Fc Binding Protein (FCGBP)" International Journal of Molecular Sciences 20, no. 20: 5000. https://doi.org/10.3390/ijms20205000