Anti-Apoptosis and Anti-Fibrosis Effects of Eriobotrya Japonica in Spontaneously Hypertensive Rat Hearts
Abstract
:1. Introduction
2. Results
2.1. EJLE Ameliorates Cardiac Functional Characteristics in SHR Animals
2.2. EJLE Ameliorates Cardiac Apoptosis in SHR Heart
2.3. EJLE Attenuates Apoptotic Signaling Mediators in SHR Animals
2.4. EJLE Enhances Survival Markers in SHR Animals
2.5. EJLE Ameliorates Fibrotic Phenomena in SHR Animals
2.6. EJLE Attenuates Fibrosis Associated Proteins in SHR Animals
3. Discussion
4. Materials and Methods
4.1. Chemical and Reagent
4.2. Preparation of EJLE
4.3. Animal Model and Experimental Design
4.4. Echocardiography
4.5. Masson’s Trichrome Staining
4.6. Terminal Deoxynucleotidyl Transferase-Mediated Deoxyuridine Triphosphate (dUTP) Nick End Labeling (TUNEL) and 4',6-Diamidine-2-Phenylindole Dihydrochloride (DAPI) Staining
4.7. Tissue Extraction
4.8. Western Blot
4.9. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
- Bromfield, S.; Muntner, P. High blood pressure: The leading global burden of disease risk factor and the need for worldwide prevention programs. Curr. Hypertens. Rep. 2013, 15, 134–136. [Google Scholar] [PubMed]
- Diez, J.; Fortuno, M.A.; Ravassa, S. Apoptosis in hypertensive cardiopathy. Revista Espanola de Cardiologia 1999, 52, 18–24. [Google Scholar] [PubMed]
- Huang, C.Y.; Kuo, W.W.; Yeh, Y.L.; Ho, T.J.; Lin, J.Y.; Lin, D.Y.; Chu, C.H.; Tsai, F.J.; Tsai, C.H.; Huang, C.Y. ANG II promotes IGF-IIR expression and cardiomyocyte apoptosis by inhibiting HSF1 via JNK activation and SIRT1 degradation. Cell Death Differ. 2014, 21, 1262–1274. [Google Scholar] [PubMed]
- Chu, C.H.; Lo, J.F.; Hu, W.S.; Lu, R.B.; Chang, M.H.; Tsai, F.J.; Tsai, C.H.; Weng, Y.S.; Tzang, B.S.; Huang, C.Y. Histone acetylation is essential for ANG-II-induced IGF-IIR gene expression in H9c2 cardiomyoblast cells and pathologically hypertensive rat heart. J. Cell Physiol. 2012, 227, 259–268. [Google Scholar] [CrossRef] [PubMed]
- Lee, S.D.; Chu, C.H.; Huang, E.J.; Lu, M.C.; Liu, J.Y.; Liu, C.J.; Hsu, H.H.; Lin, J.A.; Kuo, W.W.; Huang, C.Y. Roles of insulin-like growth factor II in cardiomyoblast apoptosis and in hypertensive rat heart with abdominal aorta ligation. Am. J. Physiol. Endocrinol. Metab. 2006, 291, E306–E314. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Narula, J.; Haider, N.; Virmani, R.; DiSalvo, T.G.; Kolodgie, F.D.; Hajjar, R.J.; Schmidt, U.; Semigran, M.J.; Dec, G.W.; Khaw, B.A. Apoptosis in myocytes in end-stage heart failure. N. Engl. J. Med. 1996, 335, 1182–1189. [Google Scholar] [CrossRef] [PubMed]
- Gonzalez, A.; Fortuno, M.A.; Querejeta, R.; Ravassa, S.; Lopez, B.; Lopez, N.; Diez, J. Cardiomyocyte apoptosis in hypertensive cardiomyopathy. Cardiovasc. Res. 2003, 59, 549–562. [Google Scholar] [PubMed]
- Adams, J.M.; Cory, S. Life-or-death decisions by the Bcl-2 protein family. Trends Biochem. Sci. 2001, 26, 61–66. [Google Scholar] [PubMed]
- Porter, A.G.; Janicke, R.U. Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 1999, 6, 99–104. [Google Scholar] [CrossRef] [PubMed]
- Aikawa, R.; Nawano, M.; Gu, Y.; Katagiri, H.; Asano, T.; Zhu, W.; Nagai, R.; Komuro, I. Insulin prevents cardiomyocytes from oxidative stress-induced apoptosis through activation of PI3 kinase/Akt. Circulation 2000, 102, 2873–2879. [Google Scholar] [PubMed]
- Hers, I.; Vincent, E.E.; Tavare, J.M. Akt signalling in health and disease. Cell Signal. 2011, 23, 1515–1527. [Google Scholar] [CrossRef] [PubMed]
- Yao, H.; Han, X.; Han, X. The cardioprotection of the insulin-mediated PI3K/Akt/mTOR signaling pathway. Am. J. Cardiovasc. Drugs 2014, 14, 433–442. [Google Scholar] [CrossRef] [PubMed]
- Azevedo, P.S.; Polegato, B.F.; Minicucci, M.F.; Paiva, S.A.; Zornoff, L.A. Cardiac Remodeling: Concepts, Clinical Impact, Pathophysiological Mechanisms and Pharmacologic Treatment. Arquivos Brasileiros de Cardiologia 2016, 106, 62–69. [Google Scholar] [CrossRef] [PubMed]
- Lopez, B.; Gonzalez, A.; Ravassa, S.; Beaumont, J.; Moreno, M.U.; San Jose, G.; Querejeta, R.; Diez, J. Circulating Biomarkers of Myocardial Fibrosis: The Need for a Reappraisal. J. Am. Coll. Cardiol. 2015, 65, 2449–2456. [Google Scholar] [CrossRef] [PubMed]
- Leask, A. Getting to the heart of the matter: New insights into cardiac fibrosis. Circ. Res. 2015, 116, 1269–1276. [Google Scholar] [CrossRef] [PubMed]
- Chang, M.H.; Kuo, W.W.; Chen, R.J.; Lu, M.C.; Tsai, F.J.; Kuo, W.H.; Chen, L.Y.; Wu, W.J.; Huang, C.Y.; Chu, C.H. IGF-II/mannose 6-phosphate receptor activation induces metalloproteinase-9 matrix activity and increases plasminogen activator expression in H9c2 cardiomyoblast cells. J. Mol. Endocrinol. 2008, 41, 65–74. [Google Scholar] [CrossRef] [PubMed]
- Gupta, K.K.; Donahue, D.L.; Sandoval-Cooper, M.J.; Castellino, F.J.; Ploplis, V.A. Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation. Sci. Rep. 2017, 7, 365. [Google Scholar] [CrossRef] [PubMed]
- Knier, B.; Cordasic, N.; Klanke, B.; Heusinger-Ribeiro, J.; Daniel, C.; Veelken, R.; Hartner, A.; Hilgers, K.F. Effect of the plasminogen-plasmin system on hypertensive renal and cardiac damage. J. Hypertens. 2011, 29, 1602–1612. [Google Scholar] [CrossRef] [PubMed]
- Eisenberg, D.M.; Davis, R.B.; Ettner, S.L.; Appel, S.; Wilkey, S.; van Rompay, M.; Kessler, R.C. Trends in alternative medicine use in the United States, 1990–1997: Results of a follow-up national survey. JAMA 1998, 280, 1569–1575. [Google Scholar] [CrossRef] [PubMed]
- Ciou, S.Y.; Hsu, C.C.; Kuo, Y.H.; Chao, C.Y. Effect of wild bitter gourd treatment on inflammatory responses in BALB/c mice with sepsis. BioMedicine 2014, 4, 17. [Google Scholar] [CrossRef] [PubMed]
- Chiu, W.C.; Yang, H.H.; Chiang, S.C.; Chou, Y.X.; Yang, H.T. Auricularia polytricha aqueous extract supplementation decreases hepatic lipid accumulation and improves antioxidative status in animal model of nonalcoholic fatty liver. BioMedicine 2014, 4, 12. [Google Scholar] [CrossRef] [PubMed]
- Xiong, X.; Yang, X.; Liu, Y.; Zhang, Y.; Wang, P.; Wang, J. Chinese herbal formulas for treating hypertension in traditional Chinese medicine: Perspective of modern science. Hypertens. Res. 2013, 36, 570–579. [Google Scholar] [CrossRef] [PubMed]
- Xiong, X.; Yang, X.; Duan, L.; Liu, W.; Zhang, Y.; Liu, Y.; Wang, P.; Li, S.; Li, X. Traditional Chinese medicine suppresses left ventricular hypertrophy by targeting extracellular signal-regulated kinases signaling pathway in spontaneously hypertensive rats. Sci. Rep. 2017, 7, 42965. [Google Scholar] [CrossRef] [PubMed]
- Xiong, X. Integrating traditional Chinese medicine into Western cardiovascular medicine: An evidence-based approach. Nat. Rev. Cardiol. 2015, 12, 374. [Google Scholar] [CrossRef] [PubMed]
- Xiong, X.; Borrelli, F.; de Sa Ferreira, A.; Ashfaq, T.; Feng, B. Herbal medicines for cardiovascular diseases. Evid Based Complement Alternat Med. 2014, 2014, 809741. [Google Scholar] [CrossRef] [PubMed]
- Wang, J.; Xiong, X. Evidence-based chinese medicine for hypertension. Evid. Based Complement. Altern. Med. 2013, 2013, 978398. [Google Scholar] [CrossRef] [PubMed]
- Qi, J.; Yu, J.; Tan, Y.; Chen, R.; Xu, W.; Chen, Y.; Lu, J.; Liu, Q.; Wu, J.; Gu, W.; et al. Mechanisms of Chinese Medicine Xinmailong’s protection against heart failure in pressure-overloaded mice and cultured cardiomyocytes. Sci. Rep. 2017, 7, 42843. [Google Scholar] [CrossRef] [PubMed]
- Tsai, C.T.; Chang, Y.M.; Lin, S.L.; Chen, Y.S.; Yeh, Y.L.; Padma, V.V.; Tsai, C.C.; Chen, R.J.; Ho, T.J.; Huang, C.Y. Alpinate Oxyphyllae Fructus Inhibits IGFII-Related Signaling Pathway to Attenuate Ang II-Induced Pathological Hypertrophy in H9c2 Cardiomyoblasts. J.Med. Food 2016, 19, 300–309. [Google Scholar] [CrossRef] [PubMed]
- Lin, Y.C.; Lin, C.H.; Yao, H.T.; Kuo, W.W.; Shen, C.Y.; Yeh, Y.L.; Ho, T.J.; Padma, V.V.; Lin, Y.C.; Huang, C.Y.; et al. Platycodon grandiflorum (PG) reverses angiotensin II-induced apoptosis by repressing IGF-IIR expression. J. Ethnopharmacol. 2017, 205, 41–50. [Google Scholar] [CrossRef] [PubMed]
- Huang, C.Y.; Kuo, W.W.; Kuo, C.H.; Tsai, F.J.; Liu, P.Y.; Hsieh, D.J. Protective effect of Danggui (Radix Angelicae Sinensis) on angiotensin II-induced apoptosis in H9c2 cardiomyoblast cells. BMC Complement. Altern. Med. 2014, 14, 358. [Google Scholar] [CrossRef] [PubMed]
- Tsai, K.H.; Lee, N.H.; Chen, G.Y.; Hu, W.S.; Tsai, C.Y.; Chang, M.H.; Jong, G.P.; Kuo, C.H.; Tzang, B.S.; Tsai, F.J.; et al. Dung-shen (Codonopsis pilosula) attenuated the cardiac-impaired insulin-like growth factor II receptor pathway on myocardial cells. Food Chem. 2013, 138, 1856–1867. [Google Scholar] [CrossRef] [PubMed]
- Cha, D.S.; Eun, J.S.; Jeon, H. Anti-inflammatory and antinociceptive properties of the leaves of Eriobotrya japonica. J. Ehnopharmacol. 2011, 134, 305–312. [Google Scholar] [CrossRef] [PubMed]
- Liu, Y.; Zhang, W.; Xu, C.; Li, X. Biological Activities of Extracts from Loquat (Eriobotrya japonica Lindl.): A Review. Int. J. Mol. Sci. 2016, 17, 983. [Google Scholar] [CrossRef] [PubMed]
- Maher, K.; Yassine, B.A.; Sofiane, B. Anti-inflammatory and antioxidant properties of Eriobotrya japonica leaves extracts. Afr. Health Sci. 2015, 15, 613–620. [Google Scholar] [CrossRef] [PubMed]
- Kyrylkova, K.; Kyryachenko, S.; Leid, M.; Kioussi, C. Detection of apoptosis by TUNEL assay. Methods Mol. Biol. 2012, 887, 41–47. [Google Scholar] [PubMed]
- Kearney, P.M.; Whelton, M.; Reynolds, K.; Muntner, P.; Whelton, P.K.; He, J. Global burden of hypertension: Analysis of worldwide data. Lancet 2005, 365, 217–223. [Google Scholar] [CrossRef]
- Noh, K.K.; Chung, K.W.; Sung, B.; Kim, M.J.; Park, C.H.; Yoon, C.; Choi, J.S.; Kim, M.K.; Kim, C.M.; Kim, N.D.; et al. Loquat (Eriobotrya japonica) extract prevents dexamethasone-induced muscle atrophy by inhibiting the muscle degradation pathway in Sprague Dawley rats. Mol. Med. Rep. 2015, 12, 3607–3614. [Google Scholar] [CrossRef] [PubMed]
- Sung, B.; Hwang, S.Y.; Kim, M.J.; Kim, M.; Jeong, J.W.; Kim, C.M.; Chung, H.Y.; Kim, N.D. Loquat leaf extract enhances myogenic differentiation, improves muscle function and attenuates muscle loss in aged rats. Int. J. Mol. Med. 2015, 36, 792–800. [Google Scholar] [CrossRef] [PubMed]
- Tan, B.X.; Yang, L.; Huang, Y.Y.; Chen, Y.Y.; Peng, G.T.; Yu, S.; Wu, Y.N.; Luo, H.B.; He, X.X. Bioactive triterpenoids from the leaves of Eriobotrya japonica as the natural PDE4 inhibitors. Nat. Prod. Res. 2017, 31, 2836–2841. [Google Scholar] [CrossRef] [PubMed]
- Zhang, S.; Li, G.; Fu, X.; Qi, Y.; Li, M.; Lu, G.; Hu, J.; Wang, N.; Chen, Y.; Bai, Y.; et al. PDCD5 protects against cardiac remodeling by regulating autophagy and apoptosis. Biochem. Biophys. Res. Commun. 2015, 461, 321–328. [Google Scholar] [CrossRef] [PubMed]
- Cheng, Y.F.; Chang, Y.T.; Chen, W.H.; Shih, H.C.; Chen, Y.H.; Shyu, B.C.; Chen, C.C. Cardioprotection induced in a mouse model of neuropathic pain via anterior nucleus of paraventricular thalamus. Nat. Commun. 2017, 8, 826. [Google Scholar] [CrossRef] [PubMed]
- Salloum, F.N.; Chau, V.Q.; Hoke, N.N.; Kukreja, R.C. Tadalafil prevents acute heart failure with reduced ejection fraction in mice. Cardiovasc. Drugs Ther. 2014, 28, 493–500. [Google Scholar] [CrossRef] [PubMed]
- Liu, Y.; Qi, H.; Wang, Y.; Wu, M.; Cao, Y.; Huang, W.; Li, L.; Ji, Z.; Sun, H. Allicin protects against myocardial apoptosis and fibrosis in streptozotocin-induced diabetic rats. Phytomedicine Int. J. Phytother. Phytopharmacol. 2012, 19, 693–698. [Google Scholar] [CrossRef] [PubMed]
- McKleroy, W.; Lee, T.H.; Atabai, K. Always cleave up your mess: Targeting collagen degradation to treat tissue fibrosis. Am. J. Physiol. Lung Cell. Mol. Physiol. 2013, 304, L709–L721. [Google Scholar] [CrossRef] [PubMed]
- Huang, C.Y.; Kuo, W.W.; Lo, J.F.; Ho, T.J.; Pai, P.Y.; Chiang, S.F.; Chen, P.Y.; Tsai, F.J.; Tsai, C.H.; Huang, C.Y. Doxorubicin attenuates CHIP-guarded HSF1 nuclear translocation and protein stability to trigger IGF-IIR-dependent cardiomyocyte death. Cell Death Dis. 2016, 7, e2455. [Google Scholar] [CrossRef] [PubMed]
- Lang, R.M.; Bierig, M.; Devereux, R.B.; Flachskampf, F.A.; Foster, E.; Pellikka, P.A.; Picard, M.H.; Roman, M.J.; Seward, J.; Shanewise, J.S.; et al. Recommendations for chamber quantification: A report from the American Society of Echocardiography’s Guidelines and Standards Committee and the Chamber Quantification Writing Group, developed in conjunction with the European Association of Echocardiography, a branch of the European Society of Cardiology. J. Am. Soc. Echocardiogr. 2005, 18, 1440–1463. [Google Scholar] [PubMed]
- Lin, P.P.; Hsieh, Y.M.; Kuo, W.W.; Lin, Y.M.; Yeh, Y.L.; Lin, C.C.; Tsai, F.J.; Tsai, C.H.; Huang, C.Y.; Tsai, C.C. Probiotic-fermented purple sweet potato yogurt activates compensatory IGFIR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats. Int. J. Mol. Med. 2013, 32, 1319–1328. [Google Scholar] [CrossRef] [PubMed]
- Chen, P.Y.; Hou, C.W.; Shibu, M.A.; Day, C.H.; Pai, P.; Liu, Z.R.; Lin, T.Y.; Viswanadha, V.P.; Kuo, C.H.; Huang, C.Y. Protective effect of Co-enzyme Q10 On doxorubicin-induced cardiomyopathy of rat hearts. Environ. Toxicol. 2017, 32, 679–689. [Google Scholar] [CrossRef] [PubMed]
- Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; Randall, R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193, 265–275. [Google Scholar] [PubMed]
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Chiang, J.-T.; Badrealam, K.F.; Shibu, M.A.; Cheng, S.-F.; Shen, C.-Y.; Chang, C.-F.; Lin, Y.-M.; Viswanadha, V.P.; Liao, S.-C.; Huang, C.-Y. Anti-Apoptosis and Anti-Fibrosis Effects of Eriobotrya Japonica in Spontaneously Hypertensive Rat Hearts. Int. J. Mol. Sci. 2018, 19, 1638. https://doi.org/10.3390/ijms19061638
Chiang J-T, Badrealam KF, Shibu MA, Cheng S-F, Shen C-Y, Chang C-F, Lin Y-M, Viswanadha VP, Liao S-C, Huang C-Y. Anti-Apoptosis and Anti-Fibrosis Effects of Eriobotrya Japonica in Spontaneously Hypertensive Rat Hearts. International Journal of Molecular Sciences. 2018; 19(6):1638. https://doi.org/10.3390/ijms19061638
Chicago/Turabian StyleChiang, Jui-Ting, Khan Farheen Badrealam, Marthandam Asokan Shibu, Sue-Fei Cheng, Chia-Yao Shen, Chih-Feng Chang, Yueh-Min Lin, Vijaya Padma Viswanadha, Shih-Chieh Liao, and Chih-Yang Huang. 2018. "Anti-Apoptosis and Anti-Fibrosis Effects of Eriobotrya Japonica in Spontaneously Hypertensive Rat Hearts" International Journal of Molecular Sciences 19, no. 6: 1638. https://doi.org/10.3390/ijms19061638