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High-Throughput 2018, 7(2), 10; https://doi.org/10.3390/ht7020010

Red Blood Cell Agglutination for Blood Typing Within Passive Microfluidic Biochips

1
University Grenoble Alpes, F-38000 Grenoble, France
2
CEA LETI MlNATEC Campus, F-38054 Grenoble, France
3
University Grenoble Alpes, CEA, CNRS, INAC, SyMMES, F-38000 Grenoble, France
*
Author to whom correspondence should be addressed.
Received: 22 March 2018 / Revised: 16 April 2018 / Accepted: 16 April 2018 / Published: 19 April 2018
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Abstract

Pre-transfusion bedside compatibility test is mandatory to check that the donor and the recipient present compatible groups before any transfusion is performed. Although blood typing devices are present on the market, they still suffer from various drawbacks, like results that are based on naked-eye observation or difficulties in blood handling and process automation. In this study, we addressed the development of a red blood cells (RBC) agglutination assay for point-of-care blood typing. An injection molded microfluidic chip that is designed to enhance capillary flow contained anti-A or anti-B dried reagents inside its microchannel. The only blood handling step in the assay protocol consisted in the deposit of a blood drop at the tip of the biochip, and imaging was then achieved. The embedded reagents were able to trigger RBC agglutination in situ, allowing for us to monitor in real time the whole process. An image processing algorithm was developed on diluted bloods to compute real-time agglutination indicator and was further validated on undiluted blood. Through this proof of concept, we achieved efficient, automated, real time, and quantitative measurement of agglutination inside a passive biochip for blood typing which could be further generalized to blood biomarker detection and quantification. View Full-Text
Keywords: quantitative agglutination assay; passive microfluidic biochip; embedded reagents; automated image processing; real time detection; blood typing; Point-of-Care quantitative agglutination assay; passive microfluidic biochip; embedded reagents; automated image processing; real time detection; blood typing; Point-of-Care
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Huet, M.; Cubizolles, M.; Buhot, A. Red Blood Cell Agglutination for Blood Typing Within Passive Microfluidic Biochips. High-Throughput 2018, 7, 10.

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