Revolution in Evolution of Human “Anellome” †
Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
†
Presented at Viruses 2020—Novel Concepts in Virology, Barcelona, Spain, 5–7 February 2020.
Proceedings 2020, 50(1), 50; https://doi.org/10.3390/proceedings2020050050
Published: 15 June 2020
(This article belongs to the Proceedings of Viruses 2020—Novel Concepts in Virology)
Abstract
:Torque teno viruses (TTVs), the most well-known members of the family Anelloviridae, are not associated with any disease. They are one of the most abundant and divergent entities in the viral world; however, the cause of their variability is not currently known. In this study, a set of longitudinally collected serum samples from two HIV-1 infected and two non-infected persons was analyzed for the presence of TTVs and other Anelloviridae using a genera-specific quantitative PCR. The samples positive for TTVs were selected for the quantitative heteroduplex tracking assay (QHTA), which showed repeating patterns of TTV genotypes. Sanger sequencing of the partial viral sequences revealed that the same strains of TTVs are most probably disappearing and returning in different stages of life, with scarcely any new introductions. The partial sequences were grouped into phylogenetic genogroups, and samples representing each of these genogroups were selected for full-length genome sequencing using an in-house optimized rolling circle amplification (RCA)–Illumina protocol.
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MDPI and ACS Style
Kaczorowska, J.; Sparreboom, M.; Deijs, M.; Jebbink, M.F.; Hoek, L.v.d. Revolution in Evolution of Human “Anellome”. Proceedings 2020, 50, 50. https://doi.org/10.3390/proceedings2020050050
AMA Style
Kaczorowska J, Sparreboom M, Deijs M, Jebbink MF, Hoek Lvd. Revolution in Evolution of Human “Anellome”. Proceedings. 2020; 50(1):50. https://doi.org/10.3390/proceedings2020050050
Chicago/Turabian StyleKaczorowska, Joanna, Mila Sparreboom, Martin Deijs, Maarten F. Jebbink, and Lia van der Hoek. 2020. "Revolution in Evolution of Human “Anellome”" Proceedings 50, no. 1: 50. https://doi.org/10.3390/proceedings2020050050
