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Proceedings 2017, 1(4), 543; doi:10.3390/proceedings1040543

Dielectric Characterisation of Single Microalgae Cell Using Electrorotation Measurements

1
Department of Engineering and System Science, National Tsing Hua University, Hsinchu City 300, Taiwan
2
Department of Mechanical Engineering, National Taiwan University, Hsinchu City 300, Taiwan
3
SATIE, Ecole normale Supérieure Paris-Saclay, 94230 Cachan, France
4
LGPM, Ecole Centrale-Supelec, France
5
ESIEE Paris, ESYCOM, Université Paris Este, 77420 Champs-sur-Marne, France
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 7 August 2017
Download PDF [700 KB, uploaded 4 September 2017]

Abstract

This study demonstrates the electrorotation of single microalgae cells captured in the center of a set of planar microelectrodes within a microfluidic device, and compares the rotational speeds of single microalgae cells obtained in experiments and theoretical calculations. Negative dielectrophoresis force and rotational electric field are generated by the microelectrode set to position and rotate single microalgae cells. The rotational speed of single microalgae cells in the microfluidic device is calculated automatically using an image-processing algorithm. The electrorotation spectra of microalgae cells enduring different periods of nitrogen starvation agree with the simulation results for low- and high-lipid content cells. These results show the great potential of using electrorotation in quantifying the dielectric characteristics of microalgae cells.
Keywords: microfluidics; electrorotation; microalgae; dielectric characteristics microfluidics; electrorotation; microalgae; dielectric characteristics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lin, Y.-S.; Tsang, S.; Ghasemi, R.; Bensalem, S.; Français, O.; Lopes, F.; Wang, H.-Y.; Sun, C.-L.; Pioufle, B.L. Dielectric Characterisation of Single Microalgae Cell Using Electrorotation Measurements. Proceedings 2017, 1, 543.

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