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Proceedings 2017, 1(4), 538; doi:10.3390/proceedings1040538

Cortisol Stress Biosensor Based on Molecular Imprinted Polymer

1
Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
2
Molecular Recognition Materials Research Unit, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand
3
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Khon Kaen University, Khon Kaen 40002, Thailand
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 16 August 2017
(This article belongs to the Proceedings of Eurosensors 2017)
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Abstract

The molecular imprinted polymer (MIP) technique was applied to receptors that could imitate the performance of natural anti-cortisol antibody with high stability. Cortisol-molecular imprinted polymer (cortisol-MIP) was obtained using the multi-step swelling and polymerization protocol with cortisol as a target molecule. The suitable ratio of methacrylic acid:acrylamide :cortisol which allowed the formation of 642.3 ± 35.8 nm homogenous particles was 2:1:1. Cortisol-MIP was integrated to the sensor. It was coated on the disposable screen-printed carbon electrode (SPCE) for portable electrochemical analysis. The physical properties of Cortisol-MIP were characterized by means of electron microscope techniques. The binding characteristics were evaluated via covalent patterns changing in FTIR spectra which were related to voltammetry responses. The SPCE modified with cortisol-MIP was able to perform various detection ranges from 1.28 to 20 nM and the high selectivity compared to three other glucocorticoid analogs.
Keywords: core shell molecular imprinted polymer (Cs-MIP); cortisol; screen printed carbon electrode (SPCE) core shell molecular imprinted polymer (Cs-MIP); cortisol; screen printed carbon electrode (SPCE)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Klangphukhiew, S.; Srichana, R.; Patramanon, R. Cortisol Stress Biosensor Based on Molecular Imprinted Polymer. Proceedings 2017, 1, 538.

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