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Proceedings 2017, 1(4), 534; doi:10.3390/proceedings1040534

Impedimetric IgG-Biosensor with In-Situ Generation of the Redox-Probe

1
AIT Austrian Institute of Technology GmbH, 1220 Vienna, Austria
2
Institute of Chemical Technologies and Analytics, Technische Universität Wien, 1060 Vienna, Austria
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 4 August 2017
(This article belongs to the Proceedings of Eurosensors 2017)
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Abstract

For most electrochemical impedance spectroscopy measurements Ferro-/Ferricyanide is used as redox-probe, but it has limitations in its application for biosensors based on gold electrodes because of chemical degradation induced by the Ferro-/Ferricyanide. The in-situ reduction of [Ru(NH3)6]3+ to [Ru(NH3)6]2+ by means of a direct current during EIS measurements is introduced to generate a stable redox-probe for biosensors. This method of enhanced EIS measurement has been applied to determine the charge transfer resistance of a human-IgG biosensor with a linear range from 0.9 to 50 mg/L IgG.
Keywords: biosensor; impedance spectroscopy; redox-probe; electrochemistry; immunosensor biosensor; impedance spectroscopy; redox-probe; electrochemistry; immunosensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Schrattenecker, J.D.; Heer, R.; Hainberger, R.; Fafilek, G. Impedimetric IgG-Biosensor with In-Situ Generation of the Redox-Probe. Proceedings 2017, 1, 534.

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