Next Article in Journal
Effect of Electrode Configuration on High Temperature Thickness Shear Gallium Phosphate Transducer
Previous Article in Journal
Low-Power Odor-Sensing Network Based on Wake-Up Nodes
Article Menu
Issue 4 (Eurosensors 2017) cover image

Article Versions

Export Article

Open AccessProceedings
Proceedings 2017, 1(4), 527; doi:10.3390/proceedings1040527

A Miniaturized SPLITT System for On-Line Protein Separation

1
Fondazione Bruno Kessler, Trento, Italy
2
Università di Trento, Trento, Italy
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 25 August 2017
(This article belongs to the Proceedings of Eurosensors 2017)
Download PDF [485 KB, uploaded 26 August 2017]

Abstract

The development of a microfluidic module for on-line protein separation is presented in this paper. The device is based on the SPLITT (Split flow Thin fractionation) technique and the separation of proteins is electrically driven by means of two platinum electrodes included in the structure of the microdevice. The microfluidic network is realized by means of a thin dry film structure, Ordyl SY 355, (thermo-compression lamination), laminated on three levels, which is patterned through a photolithographic technique. The device has been tested with a Bovine Serum Albumin (BSA) solution, through absorbance measurements with a spectrophotometer, with best achieved separation at the outlet of 40%, measured as relative concentration unbalance at output channels.
Keywords: sample preparation; protein separation; microfluidics; dry film; SPLITT; electrophoresis sample preparation; protein separation; microfluidics; dry film; SPLITT; electrophoresis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Capuano, A.; Adami, A.; Mulloni, V.; Lorenzelli, L. A Miniaturized SPLITT System for On-Line Protein Separation. Proceedings 2017, 1, 527.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Proceedings EISSN 2504-3900 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top