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Proceedings 2017, 1(4), 491; doi:10.3390/proceedings1040491

Label-Free Immunodetection in High Ionic Strength Solutions Using Carbon Nanotube Transistors with Nanobody Receptors

1
BioMed X Innovation Center, Heidelberg, Germany
2
Institute for Physical Chemistry, Universität Heidelberg, Heidelberg, Germany
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 25 August 2017
Download PDF [346 KB, uploaded 11 October 2017]

Abstract

Nanomaterial-based field-effect transistors (FETs) have been proposed for real-time, label-free detection of various biological species. However, screening of the analyte charge by electrolyte ions (Debye screening) has so far limited their use in physiological samples. Here, this challenge is overcome by combining FETs based on single-walled semiconducting carbon nanotube networks (SWCNTs) with a novel surface functionalization comprising: (1) short nanobody receptors, and (2) a polyethylene glycol layer (PEG). Nanobodies are stable, easy-to-produce, short biological receptors (~2–4 nm) that enable analyte binding closer to the sensor surface. The addition of PEG enhances the signal in high ionic strength environment. Using green fluorescent protein (GFP) as a model antigen, high selectivity and sub-picomolar detection limit with a dynamic range exceeding 4 orders of magnitude is demonstrated in physiological solutions. The presented immunoassay is fast, label-free, does not require any sample pre-treatment or washing steps.
Keywords: single-walled carbon nanotube (SWCNT) networks; field-effect transistor (FET); Debye screening; label-free immunosensing; nanobodies (VHH); polyethylene glycol (PEG) single-walled carbon nanotube (SWCNT) networks; field-effect transistor (FET); Debye screening; label-free immunosensing; nanobodies (VHH); polyethylene glycol (PEG)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Filipiak, M.S.; Rother, M.; Andoy, N.M.; Knudsen, A.C.; Grimm, S.B.; Bachran, C.; Swee, L.K.; Zaumseil, J.; Tarasov, A. Label-Free Immunodetection in High Ionic Strength Solutions Using Carbon Nanotube Transistors with Nanobody Receptors. Proceedings 2017, 1, 491.

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