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Proceedings 2017, 1(10), 995; doi:10.3390/proceedings1100995

Curcumin Enhances the Efficacy of 5-FU in Colo205 Cell Lines

Department of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri 38039, Turkey
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
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Published: 16 November 2017
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Abstract

5-FU is the main antimetabolite drug used in treating colon cancer. However, treatment success is only 10–15% due to drug resistance. To avoid resistance and improve treatment efficacy without increasing general toxicity, activity of combining curcumin with 5-FU in Colo205 cells was investigated for the first time with a real-time cell analyzer system. The cytotoxicity of 5-FU on Colo205 cells alone and in combination with curcumin were evaluated using the xCELLigence system. API-1 was used as positive control. Colo205 cells (25,000 cells/well) treated with 5-FU (1; 4; 8; 16; 32; 64 μM), API-1 (12.5; 25; 50 μM) and curcumin (25; 50 μM) 24 h after cell seeding. Cell viability was monitored for 48 h post-treatment and IC50 values were calculated using xCELLigence software. Concentrations used in the combination were determined as, 64 μM 5-FU, 50 μM curcumin and 25 μM API-1. Due to cytotoxic effect profile similarity between curcumin and protein kinase inhibitor API-1; when used in combination with 5-FU, curcumin was observed to increase the efficacy of 5-FU and accelerate the cytotoxic effect by removing the cytostatic period seen in the first 6 h. The study results show that the combination of 5-FU and curcumin in Colo205 can reduce the dose by increasing the cytotoxic activity of 5-FU and reducing the resistance to the anticancer drug.
Keywords: 5-FU; API-1; Colo205; curcumin; xCELLigence 5-FU; API-1; Colo205; curcumin; xCELLigence
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Öztürk, E.; Arslan, A.K.K.; Radwan, B.; Yerer, M.B. Curcumin Enhances the Efficacy of 5-FU in Colo205 Cell Lines. Proceedings 2017, 1, 995.

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