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Proceedings 2017, 1(10), 1016; doi:10.3390/proceedings1101016

The Effects of Paclitaxel and Metformin and Combined Treatments on TLR Signaling Pathway on MDA-MB-231 Breast Cancer Cell Lines

1
Faculty of Medicine, Department of Histology & Embryology, Manisa Celal Bayar University, Manisa 45047, Turkey
2
Faculty of Medicine, Department of Histology & Embryology, Izmir University of Economics, Izmir 35040, Turkey
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
*
Author to whom correspondence should be addressed.
Published: 15 November 2017
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Abstract

The aim of this study was to determine the effects of Paclitaxel, Metformin and combined treatments on human breast cancer cell line MDA-MB-231 via TLR signaling pathway using immunocytochemical technique. MDA-MB-231 breast cancer cells were cultured in RPMI-1640 medium containing 10% FBS, 1% L-glutamine and 1% penicillin/streptomycin. Anti-TLR2, anti-TLR4, anti-MyD88, anti-NFkB, anti-IL-6 and anti-ERK primary antibodies were used for indirect immunohistochemistry after 24 h administrations of Paclitaxel, Metformin and combination of them. The mean values of the staining intensities (mild, moderate, strong and very strong) and percentage of positively stained cells were calculated using H-Score. The results showed that the immunoreactivities of TLR-2, TLR-4, MyD88, NFkB and ERK is increased after the drug treatments while the immunoreactivity of IL-6 has not changed between control and treated groups. To conclude that paclitaxel, metformin and combined therapies on breast cancer cells caused the activation of the TLR-MyD88-ERK signaling pathway which mediates tumor growth and progression, metastasis and drug resistance.
Keywords: breat cancer cell line; paclitaxel; metformin; TLR breat cancer cell line; paclitaxel; metformin; TLR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ozgul, M.; Uluer, E.T.; Onal, T.; Akogullari, D.; Ozbilgin, K.; Inan, S. The Effects of Paclitaxel and Metformin and Combined Treatments on TLR Signaling Pathway on MDA-MB-231 Breast Cancer Cell Lines. Proceedings 2017, 1, 1016.

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