Next Article in Journal
Effect of Pomegranate Extract and Tangeretin on Specific Pathways in the Rat Breast Cancer Model Induced with DMBA
Previous Article in Journal
Apoptotic Effect of Ginnalin A on MDA-MB-231 and MCF7 Human Breast Cancer Cell Lines
Article Menu

Article Versions

Export Article

Open AccessAbstract
Proceedings 2017, 1(10), 1003; doi:10.3390/proceedings1101003

Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells

1
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Erciyes University, Kayseri 38039, Turkey
2
Department of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri 38039, Turkey
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
*
Author to whom correspondence should be addressed.
Published: 13 November 2017
Download PDF [139 KB, uploaded 27 November 2017]

Abstract

Colon cancer is one of the causes of cancer-related mortality. So, more efficient therapy strategies are needed. There is an increasing interest in natural products due to their potential cytotoxic activity in various cancer cell lines. Osthole and imperatorin are major active coumarins found in a variety of plants. The aim of the present study was to assess the cytotoxic effects of osthole and imperatorin administered separately and in combination with 5-fluorouracil (5-FU) in human colon carcinoma cells and to identify the action of mechanism. Therefore, Colo205 cells were treated with imperatorin (200 μM), osthole (400 μM), and 5-FU (64 μM) 24 h after cell seeding. Real-time cell analysis by xCELLigence System was used to continuously monitor the cell proliferation and viability on Colo205 cells. Furthermore, the effects of the compound on p38 MAPK acitivity and Akt mRNA levels were evaluated. As a result, osthole showed considerable anti-proliferative activity in Colo205 cells and increased the efficacy of 5-FU by accelerating the cytotoxic effect and decreasing the Akt mRNA levels and inhibiting the p38 MAPK activity. Additionally, it was observed that osthole was more effective than imperatorin inhibiting cell proliferation. The findings indicate that osthole may be a promising anti-cancer agent in the treatment of colon cancer.
Keywords: imperatorin; osthole; 5-fluorouracil; cytotoxicity; xCELLigence imperatorin; osthole; 5-fluorouracil; cytotoxicity; xCELLigence
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Eken, A.; Arslan, A.K.K.; Öztürk, E.; Ökçesiz, A.; Yerer, M.B. Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells. Proceedings 2017, 1, 1003.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Proceedings EISSN 2504-3900 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top