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Trop. Med. Infect. Dis. 2017, 2(2), 7; doi:10.3390/tropicalmed2020007

Relative Performance and Predictive Values of Plasma and Dried Blood Spots with Filter Paper Sampling Techniques and Dilutions of the Lymphatic Filariasis Og4C3 Antigen ELISA for Samples from Myanmar

1
College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns, QLD 4870, Australia
2
Department of Medical Research, Myanmar Ministry of Health and Sports, Nay Pyi Taw, Myanmar
*
Author to whom correspondence should be addressed.
Academic Editors: Thewarach Laha and Peter A. Leggat
Received: 11 February 2017 / Revised: 27 March 2017 / Accepted: 4 April 2017 / Published: 11 April 2017
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Abstract

Diagnostic testing of blood samples for parasite antigen Og4C3 is used to assess Wuchereria bancrofti in endemic populations. However, the Tropbio ELISA recommends that plasma and dried blood spots (DBS) prepared using filter paper be used at different dilutions, making it uncertain whether these two methods and dilutions give similar results, especially at low levels of residual infection or resurgence during the post-program phase. We compared results obtained using samples of plasma and DBS taken simultaneously from 104 young adults in Myanmar in 2014, of whom 50 (48.1%) were positive for filariasis antigen by rapid antigen test. Results from DBS tests at recommended dilution were significantly lower than results from plasma tested at recommended dilution, with comparisons between plasma and DBS at unmatched dilutions yielding low sensitivity and negative predictive values of 60.0% and 70.6% respectively. While collection of capillary blood on DBS is cheaper and easier to perform than collecting plasma or serum, and does not need to be stored frozen, dilutions between different versions of the test must be reconciled or an adjustment factor applied. View Full-Text
Keywords: lymphatic filariasis; Og4C3; ELISA; filter paper; DBS; ICT; immunochromatographic test lymphatic filariasis; Og4C3; ELISA; filter paper; DBS; ICT; immunochromatographic test
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MDPI and ACS Style

Masson, J.; Douglass, J.; Roineau, M.; Aye, K.S.; Htwe, K.M.; Warner, J.; Graves, P.M. Relative Performance and Predictive Values of Plasma and Dried Blood Spots with Filter Paper Sampling Techniques and Dilutions of the Lymphatic Filariasis Og4C3 Antigen ELISA for Samples from Myanmar. Trop. Med. Infect. Dis. 2017, 2, 7.

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Trop. Med. Infect. Dis. EISSN 2414-6366 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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