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J. Funct. Morphol. Kinesiol. 2016, 1(1), 69-89; doi:10.3390/jfmk1010069

Effects of Synthetic Anti-Inflammatory Sterol in CB3V-Induced Myocarditis: A Morphological Study on Heart Muscle Tissue

1
Department of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, School of Medicine, University of Catania, 95123 Catania, Italy
2
Department of Clinical and Experimental Medicine, Internal Medicine Division, School of Medicine, University of Catania, 95123 Catania, Italy
3
Yale Child Study Center, Yale School of Medicine, New Haven, CT 06510, USA
4
Hearth Center, “G.B. Morgagni” Mediterranean Foundation, Cardiology Unit, 95123 Catania, Italy
5
Department of Biomedical and Biotechnological Sciences, General Pathology Section, School of Medicine, University of Catania, 95123 Catania, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Giuseppe Musumeci
Received: 25 November 2015 / Revised: 19 January 2016 / Accepted: 2 February 2016 / Published: 18 February 2016
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Abstract

Cell-mediated immune events play a role in the pathogenesis of myocarditis provoked by Group B coxsackievirus (CVB). Studies indicated the synthetic derivative of androstene-3β,7β,17β-triol, HE3286 (17α-ethynyl-5-androstene-3β,7β,17β-triol), may ameliorate the course of immunoinflammatory and autoimmune diseases in rodents. The aim of this study was to evaluate effects of HE3286 on histological signs of CVB-induced myocarditis. BALB/c mice were infected with coxsackie B3 virus (CB3V) and treated by intraperitoneal administration of dexamethasone (Dex) or by oral gavage with HE3286 or with its vehicle, HERF405, for 18 days. Mice were sacrificed and hearts were explanted for histological and immunohistochemical analysis (TNF-α, IL-6, MMP9, ADAM10 and HSP-70). Heart tissues of Dex-treated mice showed a better histological structure compared with mice treated with HERF405. An almost complete resolution of myocarditis was observed in HE3286-treated mice as evidenced by lack of inflammatory infiltration. Immunohistochemical findings confirmed HE3286 had a more pronounced effect than Dex in reducing inflammatory response associated with in situ modulation of cytokine expression and tissue remodeling. Our data demonstrate HE3286 has better results in inhibiting establishment and progression of murine CVB-induced myocarditis than Dex, suggesting this drug may also have a therapeutic role in treatment of CVB-induced myocarditis. View Full-Text
Keywords: myocarditis; heart muscle tissue; coxsackie virus; HE3286; histology; immunohistochemistry myocarditis; heart muscle tissue; coxsackie virus; HE3286; histology; immunohistochemistry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Castrogiovanni, P.; Trovato, F.M.; Szychlinska, M.A.; Loreto, C.; Giunta, S.; Scuderi, S.; Passanisi, R.; Fidone, F.; Fagone, P.; Imbesi, R.; Nicoletti, F.; Castorina, S. Effects of Synthetic Anti-Inflammatory Sterol in CB3V-Induced Myocarditis: A Morphological Study on Heart Muscle Tissue. J. Funct. Morphol. Kinesiol. 2016, 1, 69-89.

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