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Inventions 2018, 3(1), 11; doi:10.3390/inventions3010011

A Simple Setup to Perform 3D Locomotion Tracking in Zebrafish by Using a Single Camera

1
Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
2
Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan
3
Center of Nanotechnology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
4
Center of Biomedical Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 6 January 2018 / Revised: 28 January 2018 / Accepted: 31 January 2018 / Published: 2 February 2018
(This article belongs to the Section Inventions and innovation in Biotechnology and Materials)
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Abstract

Generally, the measurement of three-dimensional (3D) swimming behavior in zebrafish relies on commercial software or requires sophisticated scripts, and depends on more than two cameras to capture the video. Here, we establish a simple and economic apparatus to detect 3D locomotion in zebrafish, which involves a single camera capture system that records zebrafish movement in a specially designed water tank with a mirror tilted at 45 degrees. The recorded videos are analyzed using idTracker, while spatial positions are calibrated by ImageJ software and 3D trajectories are plotted by Origin 9.1 software. This easy setting allowed scientists to track 3D swimming behavior of multiple zebrafish with low cost and precise spatial position, showing great potential for fish behavioral research in the future. View Full-Text
Keywords: 3D locomotion; behavior; zebrafish; idTracker; ImageJ 3D locomotion; behavior; zebrafish; idTracker; ImageJ
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Audira, G.; Sampurna, B.P.; Juniardi, S.; Liang, S.-T.; Lai, Y.-H.; Hsiao, C.-D. A Simple Setup to Perform 3D Locomotion Tracking in Zebrafish by Using a Single Camera. Inventions 2018, 3, 11.

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