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Inventions 2017, 2(1), 3; doi:10.3390/inventions2010003

Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection

1
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan
2
Department of Mechanical Engineering, National Taiwan University, Taipei 10617, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Shoou-Jinn Chang
Received: 2 December 2016 / Revised: 25 January 2017 / Accepted: 3 February 2017 / Published: 8 February 2017
(This article belongs to the Collection Feature Innovation Papers)
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Abstract

Polymerase chain reaction (PCR) has been one of the principal techniques of molecular biology and diagnosis for decades. Conventional PCR platforms, which work by rapidly heating and cooling the whole vessel, need complicated hardware designs, and cause energy waste and high cost. On the other hand, partial heating on the various locations of vessels to induce convective solution flows by buoyancy have been used for DNA amplification in recent years. In this research, we develop a new convective PCR platform, capillary loop convective polymerase chain reaction (clcPCR), which can generate one direction flow and make the PCR reaction more stable. The U-shaped loop capillaries with 1.6 mm inner diameter are designed as PCR reagent containers. The clcPCR platform utilizes one isothermal heater for heating the bottom of the loop capillary and a CCD device for detecting real-time amplifying fluorescence signals. The stable flow was generated in the U-shaped container and the amplification process could be finished in 25 min. Our experiments with different initial concentrations of DNA templates demonstrate that clcPCR can be applied for precise quantification. Multiple sample testing and real-time quantification will be achieved in future studies. View Full-Text
Keywords: convective polymerase chain reaction; capillary loop convective polymerase chain reaction; fluorescence detection convective polymerase chain reaction; capillary loop convective polymerase chain reaction; fluorescence detection
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MDPI and ACS Style

Chou, W.-P.; Lee, C.; Hsu, Z.-J.; Lai, M.-H.; Kuo, L.-S.; Chen, P.-H. Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection. Inventions 2017, 2, 3.

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