Agarose Cryogels: Production Process Modeling and Structural Characterization
Abstract
:1. Introduction
2. Results and Discussion
2.1. Cryogel Formation
2.2. Process Temperature Modeling
2.3. Porosity Estimation of Cryogels
2.4. Mechanical Testing and Image Analysis
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Production Method of Agarose Gels
4.3. Thermal Profiles during the Formation of Cryogels
4.4. Mechanical Test and Image Analysis
5. Modeling
5.1. System Description
5.2. Properties of Materials
5.3. Model Equations
- looking at the boundary at r = 0, for each z, the axisymmetric condition was applied;
- a “Slip” condition was applied to the interface, so ; while on all other walls, u = 0;
- the initial velocity of the fluids was zero;
- the walls were adiabatic;
- a constant temperature of 15 °C was set on the wall and a constant temperature equal to the set point of the cooler, , was set on the wall;
- the known initial temperature, or the one measured at the start of the experiment, was set on the domains: = 10 °C, + 2 °C), = 25 °C, = 85 °C, = 25 °C, = 95 °C, and ;
- on the bottom of the chiller, a specific constraint was imposed on the pressure, specifying that it was constant and equal to the hydrostatic pressure , where is the gravitational acceleration and is the height;
- crystallization could not take place until the average crystallization start temperature was reached. This statement was appropriate because the temperature at which the crystallization began depended on many factors; it was not always the same and was difficult to predict. However, it is also true that, provided that only the operating conditions of the system were changed, it was possible to identify a temperature range in which crystallization occurred and hence the definition of ;
- the properties of the agarose cryogels were the same as water. Since the aim was to model the cryogel production process and the cryogels produced were 98% water, this assumption is reasonable. Moreover, the average freezing times of the water and agarose cryogels, obtained experimentally and shown in Figure 9, confirm this assumption. Here, the average freezing time was calculated as the difference between the time when the crystallization began and the time when the sample was completely frozen.
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variable | Unit of Measurement | Description |
---|---|---|
kg/m3 | Fluid density | |
J/kg | Specific heat capacity of the solid phase | |
J/kg | Specific heat capacity of the liquid phase | |
J/kg | Specific heat capacity | |
J/(m3·s) | Power per unit volume due to the latent heat of fusion | |
J/(m3·s) | Power per unit volume due to nucleation | |
K | Temperature of subcooled solution | |
K | Temperature of fluid freezing front | |
K | Interface temperature between the mold and the agarose solution | |
kg/(m3·s) | Nucleation rate per unit volume | |
- | Volume fraction of the solid phase | |
- | Volume fraction of the liquid phase | |
J/(m·K·s) | Thermal conductivity of the solid phase | |
J/(m·K·s) | Thermal conductivity of the liquid phase | |
kg/m3 | Density of the solid phase | |
kg/m3 | Density of the liquid phase | |
T | K | Temperature |
Pa | Pressure | |
s | Time | |
m/s | Fluid velocity | |
- | Identity matrix | |
Pa | Stress tensor | |
m2/s | Gravitational acceleration | |
m | Height | |
J/(m2·s) | Conductive heat flux | |
J/(m·K·s) | Thermal conductivity | |
J/kg | Latent heat due to phase change | |
Pa·s | Fluid dynamic viscosity | |
Pa·s | Turbulent fluid dynamic viscosity | |
m2/s2 | Turbulent kinetic energy | |
ε | m2/s3 | Turbulent kinetic dissipation rate |
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Mancino, R.; Caccavo, D.; Barba, A.A.; Lamberti, G.; Biasin, A.; Cortesi, A.; Grassi, G.; Grassi, M.; Abrami, M. Agarose Cryogels: Production Process Modeling and Structural Characterization. Gels 2023, 9, 765. https://doi.org/10.3390/gels9090765
Mancino R, Caccavo D, Barba AA, Lamberti G, Biasin A, Cortesi A, Grassi G, Grassi M, Abrami M. Agarose Cryogels: Production Process Modeling and Structural Characterization. Gels. 2023; 9(9):765. https://doi.org/10.3390/gels9090765
Chicago/Turabian StyleMancino, Raffaele, Diego Caccavo, Anna Angela Barba, Gaetano Lamberti, Alice Biasin, Angelo Cortesi, Gabriele Grassi, Mario Grassi, and Michela Abrami. 2023. "Agarose Cryogels: Production Process Modeling and Structural Characterization" Gels 9, no. 9: 765. https://doi.org/10.3390/gels9090765