Exploring Biocontrol of Unwanted Fungi by Autochthonous Debaryomyces hansenii Strains Isolated from Dry Meat Products
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Primary Screening: Pathogenic Fungi Colonization Prevention Assay with Biofilms from D. hansenii Strains
2.3. Biotyping
2.4. Secondary Screening: Streak Inhibition Assay
2.5. Radial Inhibition Assay on Solid Media
2.6. Volatile Compounds Inhibition Assay
2.7. Data Quantification and Statistical Analysis
3. Results
3.1. Primary Screening
3.2. Biotyping
3.3. Secondary Screening: Streak Inhibition Assay
3.4. Radial Inhibition Assay
3.5. Volatile Compounds Inhibition Assay
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolation Origin | Debaryomyces hansenii Strains | Aspergillus niger | Penicillium expansum | Penicillium verrucosum | Candida albicans |
---|---|---|---|---|---|
Laboratory control | CBS767 | + + + | + + + | + + + | + + + |
“Chorizo Ibérico” | CB1 | + + + | + + + | + + + | + + + |
CB2 | + | + + + | + + + | + + + | |
CRC1 | + | + + + | + + + | + + + | |
CRC2 | + | + + + | + + + | + + + | |
CRF1 | + + | + + + | + + + | + + + | |
CRF2 | + + | + + + | + + + | + + + | |
CRF3 | + | + | + | + | |
“Lomo Ibérico” | LB1 | + + + | + + + | + + + | + + + |
LB2 | + + + | + + + | + + + | + + + | |
LB3 | + | + + + | + + + | + + + | |
LR1 | + + + | + + + | + + + | + + + | |
LR2 | + + + | + + + | + + + | + + + | |
LRB1 | + + + | + + + | + + + | + + + | |
LRB2 | + + + | + + + | + + + | + + + | |
LRB3 | + + + | + + + | + + + | + + + | |
LRB4 | + + + | + + + | + + + | + + + | |
LRC1 | + + + | + + + | + + + | + + + | |
LRC2 | + + + | + + + | + + + | + + + | |
LRF1 | + + | + + + | + + + | + + + | |
LRF2 | + + + | + + + | + | + | |
“Salchichón Ibérico” | SRC1 | + + + | + + + | + + + | + + + |
SRC2 | + + + | + + + | + + + | + + + | |
SRF1 | + + + | + + + | + + + | + + + |
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Chacón-Navarrete, H.; Ruiz-Pérez, F.; Ruiz-Castilla, F.J.; Ramos, J. Exploring Biocontrol of Unwanted Fungi by Autochthonous Debaryomyces hansenii Strains Isolated from Dry Meat Products. J. Fungi 2022, 8, 873. https://doi.org/10.3390/jof8080873
Chacón-Navarrete H, Ruiz-Pérez F, Ruiz-Castilla FJ, Ramos J. Exploring Biocontrol of Unwanted Fungi by Autochthonous Debaryomyces hansenii Strains Isolated from Dry Meat Products. Journal of Fungi. 2022; 8(8):873. https://doi.org/10.3390/jof8080873
Chicago/Turabian StyleChacón-Navarrete, Helena, Francisco Ruiz-Pérez, Francisco J. Ruiz-Castilla, and José Ramos. 2022. "Exploring Biocontrol of Unwanted Fungi by Autochthonous Debaryomyces hansenii Strains Isolated from Dry Meat Products" Journal of Fungi 8, no. 8: 873. https://doi.org/10.3390/jof8080873