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Bioengineering 2018, 5(3), 54; https://doi.org/10.3390/bioengineering5030054

Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration

1
Department of Periodontics and Oral Implantology, PMS College of Dental Science and Research Centre, Trivandrum 695028, India
2
Department of Endodontics and Implant Dentistry, S.U.T.A.M.S Medical College, Trivandrum 695028, India
3
Department of Orthodontics and Dentofacial Orthopedics, PMS College of Dental Science and Research Centre, Trivandrum 695028, India
4
Department of Community Dentistry, PMS College of Dental Science and Research Centre, Trivandrum 695028, India
5
Department of Oral and Maxillofacial Surgery, PSM College of Dental Science and Research, Thrissur 680519, India
6
Department of Oral and Maxillofacial Surgery, Amrita School of Dentistry, Cochin 682041, India
7
Department of Prosthodontics, PMS College of Dental Science and Research Centre, Trivandrum 695028, India
*
Author to whom correspondence should be addressed.
Received: 4 June 2018 / Revised: 5 July 2018 / Accepted: 8 July 2018 / Published: 15 July 2018
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Abstract

Background: Current resorbable and non-resorbable membranes act as a physical barrier to avoid connective and epithelial tissue downgrowth into the defect, favoring the regeneration of periodontal tissues. These conventional membranes possess many structural and bio-functional limitations. We hypothesized that the next-generation of guided tissue regeneration (GTR) membranes for periodontal tissue engineering will be a biologically active, spatially designed nanofibrous biomaterial that closely mimics the native extra-cellular matrix (ECM). Methods: GTR membranes made of poly(ε-Caprolactone) with a molecular weight of 80,000 reinforced with different weight concentrations of nano-Hydroxyapatite/Bioactive glass (2%, 5%, 10%, 15%) is fabricated by the method of electrospinning. After fabrication, in vitro properties are evaluated. Results: The electrospun nanofibrous membranes possessed excellent mechanical properties initially and after one month of degradation in phosphate buffer solution (PBS). Moreover, none of the fabricated membranes were found to be cytotoxic at lower concentrations and higher concentrations. Comparing the overall properties, PCL (poly(e-caprolactone)) + BG (Bioactive glass) 2% exhibited superior cell attachment and percentage of viable cells, increased fiber and pore diameter which satisfies the ideal properties needed for GTR membranes. Conclusion: Composite nanofibrous membranes prepared by electrospinning are suitable for use as a GTR membrane and are a useful prototype for further development of a final membrane for clinical use. View Full-Text
Keywords: electrospinning; guided tissue regeneration, periodontal regeneration electrospinning; guided tissue regeneration, periodontal regeneration
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Sunandhakumari, V.J.; Vidhyadharan, A.K.; Alim, A.; Kumar, D.; Ravindran, J.; Krishna, A.; Prasad, M. Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration. Bioengineering 2018, 5, 54.

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