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Bioengineering 2017, 4(2), 51; doi:10.3390/bioengineering4020051

Development and Characterization of a Parallelizable Perfusion Bioreactor for 3D Cell Culture

1
Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
2
Institute of Chemistry, University of Graz, Heinrichstraße 28/IV, 8010 Graz, Austria
3
Translational Center, University Hospital Wurzburg, Roentgenring 11, 97070 Wuerzburg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Christoph Herwig
Received: 29 March 2017 / Revised: 11 May 2017 / Accepted: 23 May 2017 / Published: 25 May 2017
(This article belongs to the Special Issue Hybrid Modelling and Multi-Parametric Control of Bioprocesses)
View Full-Text   |   Download PDF [9004 KB, uploaded 25 May 2017]   |  

Abstract

The three dimensional (3D) cultivation of stem cells in dynamic bioreactor systems is essential in the context of regenerative medicine. Still, there is a lack of bioreactor systems that allow the cultivation of multiple independent samples under different conditions while ensuring comprehensive control over the mechanical environment. Therefore, we developed a miniaturized, parallelizable perfusion bioreactor system with two different bioreactor chambers. Pressure sensors were also implemented to determine the permeability of biomaterials which allows us to approximate the shear stress conditions. To characterize the flow velocity and shear stress profile of a porous scaffold in both bioreactor chambers, a computational fluid dynamics analysis was performed. Furthermore, the mixing behavior was characterized by acquisition of the residence time distributions. Finally, the effects of the different flow and shear stress profiles of the bioreactor chambers on osteogenic differentiation of human mesenchymal stem cells were evaluated in a proof of concept study. In conclusion, the data from computational fluid dynamics and shear stress calculations were found to be predictable for relative comparison of the bioreactor geometries, but not for final determination of the optimal flow rate. However, we suggest that the system is beneficial for parallel dynamic cultivation of multiple samples for 3D cell culture processes. View Full-Text
Keywords: perfusion bioreactor system; 3D cell culture; dynamic cultivation; fluid shear stress; computational fluid dynamics perfusion bioreactor system; 3D cell culture; dynamic cultivation; fluid shear stress; computational fluid dynamics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Egger, D.; Fischer, M.; Clementi, A.; Ribitsch, V.; Hansmann, J.; Kasper, C. Development and Characterization of a Parallelizable Perfusion Bioreactor for 3D Cell Culture. Bioengineering 2017, 4, 51.

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