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Bioengineering 2017, 4(2), 43; doi:10.3390/bioengineering4020043

In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique

1
Faculty of Electrical and Electronic Engineering, Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia
2
Biosensor and Bioengineering Laboratory, MiNT-SRC Research Center, Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia
3
Cancer Research Malaysia, 1, Jalan SS12/1A, Subang Jaya 47500, Malaysia
4
Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kota Bahru, Malaysia
5
School of Engineering, Design and Technology, Medical Engineering, University of Bradford, Bradford BD7 1DP, UK
*
Author to whom correspondence should be addressed.
Academic Editors: Julian F. Dye and Naresh Kasoju
Received: 10 April 2017 / Revised: 8 May 2017 / Accepted: 12 May 2017 / Published: 14 May 2017
(This article belongs to the Special Issue Advanced Tissue Engineering Scaffolds)
View Full-Text   |   Download PDF [10036 KB, uploaded 14 May 2017]   |  

Abstract

Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 μL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 μm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies. View Full-Text
Keywords: alginate; aerosol; microencapsulation; microtissues; keratinocytes; oral squamous cell carcinoma alginate; aerosol; microencapsulation; microtissues; keratinocytes; oral squamous cell carcinoma
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MDPI and ACS Style

Leong, W.Y.; Soon, C.F.; Wong, S.C.; Tee, K.S.; Cheong, S.C.; Gan, S.H.; Youseffi, M. In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique. Bioengineering 2017, 4, 43.

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