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Bioengineering 2016, 3(3), 19; doi:10.3390/bioengineering3030019

Multi-Response Optimization of Granaticinic Acid Production by Endophytic Streptomyces thermoviolaceus NT1, Using Response Surface Methodology

1
Microbiology and Microbial Biotechnology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore 721102, India
2
PG Department of Biotechnology, Oriental Institute of Science and Technology, Midnapore 721102, India
3
Department of Microbiology, Vidyasagar University, Midnapore 721102, India
*
Author to whom correspondence should be addressed.
Academic Editor: Christoph Herwig
Received: 5 March 2016 / Revised: 1 May 2016 / Accepted: 20 July 2016 / Published: 27 July 2016
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Abstract

Streptomyces thermoviolaceus NT1, an endophytic isolate, was studied for optimization of granaticinic acid production. It is an antimicrobial metabolite active against even drug resistant bacteria. Different media, optimum glucose concentration, initial media pH, incubation temperature, incubation period, and inoculum size were among the selected parameters optimized in the one-variable-at-a-time (OVAT) approach, where glucose concentration, pH, and temperature were found to play a critical role in antibiotic production by this strain. Finally, the Box–Behnken experimental design (BBD) was employed with three key factors (selected after OVAT studies) for response surface methodological (RSM) analysis of this optimization study.RSM analysis revealed a multifactorial combination; glucose 0.38%, pH 7.02, and temperature 36.53 °C as the optimum conditions for maximum antimicrobial yield. Experimental verification of model analysis led to 3.30-fold (61.35 mg/L as compared to 18.64 mg/L produced in un-optimized condition) enhanced granaticinic acid production in ISP2 medium with 5% inoculum and a suitable incubation period of 10 days. So, the conjugated optimization study for maximum antibiotic production from Streptomyces thermoviolaceus NT1 was found to result in significantly higher yield, which might be exploited in industrial applications. View Full-Text
Keywords: Streptomyces thermoviolaceus; granaticinic acid; antimicrobial; optimization; response surface methodology; Box–Behnken design Streptomyces thermoviolaceus; granaticinic acid; antimicrobial; optimization; response surface methodology; Box–Behnken design
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Roy, S.; Halder, S.K.; Banerjee, D. Multi-Response Optimization of Granaticinic Acid Production by Endophytic Streptomyces thermoviolaceus NT1, Using Response Surface Methodology. Bioengineering 2016, 3, 19.

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