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Medicines 2017, 4(1), 9; doi:10.3390/medicines4010009

Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage

1
Department of Biofunctional Science, Faculty Morgana Potrich, Mineiros, Goias 75830-000, Brazil
2
Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition (ICTAN–CSIC), Madrid 28040, Spain
3
Institute of Genetics and Biochemistry, Federal University of Uberlandia, Uberlandia, Minas Gerais 38400-712, Brazil
4
Department of Pharmacology, Faculty of Pharmacy, Universidad Complutense de Madrid, Madrid 28040, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: João Rocha
Received: 22 December 2016 / Revised: 27 January 2017 / Accepted: 15 February 2017 / Published: 21 February 2017
(This article belongs to the Special Issue Herbal Medicines and Functional Foods)
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Abstract

Background: Increased oxidative stress by persistent hyperglycemia is a widely accepted factor in vascular damage responsible for type 2 diabetes complications. The plant Vochysia rufa (Vr) has been used in folk medicine in Brazil for the treatment of diabetes. Thus; the protective effect of a Vr stem bark extract against a challenge by a high glucose concentration on EA.hy926 (EA) endothelial cells is evaluated. Methods: Vegetal material is extracted with distilled water by maceration and evaporated until dryness under vacuum. Then; it is isolated by capillary electrophoresis–tandem mass spectrometry. Cell viability is evaluated on EA cells treated with 0.5–100 µg/mL of the Vr extract for 24 h. The extract is diluted at concentrations of 5, 10 and 25 µg/mL and maintained for 24 h along with 30 mM of glucose to evaluate its protective effect on reduced glutathione (GSH); glutathione peroxidase (GPx) and reductase (GR) and protein carbonyl groups. Results: V. rufa stem bark is composed mainly of sugars; such as inositol; galactose; glucose; mannose; sacarose; arabinose and ribose. Treatment with Vr up to 100 µg/mL for 24 h did not affect cell viability. Treatment of EA cells with 30 mM of glucose for 24 h significantly increased the cell damage. EA cells treated with 30 mM of glucose showed a decrease of GSH concentration and increased Radical Oxygen Species (ROS) and activity of antioxidant enzymes and protein carbonyl levels; compared to control. Co-treatment of EA with 30 mM glucose plus 1–10 μg/mL Vr significantly reduced cell damage while 5–25 μg/mL Vr evoked a significant protection against the glucose insult; recovering ROS; GSH; antioxidant enzymes and carbonyls to baseline levels. Conclusion: V. rufa extract protects endothelial cells against oxidative damage by modulating ROS; GSH concentration; antioxidant enzyme activity and protein carbonyl levels. View Full-Text
Keywords: Vochysia rufa; Sapotaceae; endothelial cells; oxidative damage; plant antioxidants Vochysia rufa; Sapotaceae; endothelial cells; oxidative damage; plant antioxidants
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MDPI and ACS Style

Moura de Gouveia, N.; Ramos, S.; Martín, M.Á.; Espindola, F.S.; Goya, L.; Palomino, O.M. Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage. Medicines 2017, 4, 9.

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