Comparison of Cultivars and Seasonal Variation in Blueberry (Vaccinium Species) Leaf Extract on Adult T-Cell Leukemia Cell Line Growth Suppression
Abstract
:1. Introduction
2. Experimental Section
2.1. Plant Materials
Species | Cultivars | Voucher Number Yield (%) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
April | May | June | July | August | September | October | November | December | ||
RB | Homebell | RB121 | RB122 | RB123 | RB124 | RB125 | RB126 | RB127 | RB128 | RB129 |
48.7% | 37.1% | 40.5% | 37.1% | 39.3% | 43.9% | 40.3% | 44.7% | 41.2% | ||
Myers | RB131 | RB132 | RB133 | RB134 | RB135 | RB136 | RB137 | RB138 | RB139 | |
43.3% | 43.2% | 43.7% | 45.7% | 44.9% | 43.3% | 41.9% | 42.5% | 37.0% | ||
Tifblue | RB141 | RB142 | RB143 | RB144 | RB145 | RB146 | RB147 | RB148 | RB149 | |
39.6% | 33.2% | 38.5% | 30.8% | 36.7% | 35.2% | 34.5% | 37.0% | 34.2% |
2.2. Extraction
Species | Cultivars | Voucher Number Yield (%) | ||||
---|---|---|---|---|---|---|
April | June | August | October | December | ||
RB | Climax | RB011 | RB012 | RB013 | RB014 | RB015 |
28.8% | 34.7% | 42.0% | 44.5% | 37.7% | ||
Suwannee | RB021 | RB022 | RB023 | RB024 | RB025 | |
35.6% | 35.0% | 35.5% | 34.2% | 41.6% | ||
Myers | RB031 | RB032 | RB033 | RB034 | RB035 | |
34.1% | 42.8% | 38.5% | 40.9% | 41.6% | ||
Callaway | RB041 | RB042 | RB043 | RB044 | RB045 | |
37.3% | 39.1% | 39.9% | 39.4% | 41.9% | ||
Ethel | RB051 | RB052 | RB053 | RB054 | RB055 | |
32.0% | 40.1% | 43.4% | 39.9% | 34.5% | ||
Southland | RB061 | RB062 | RB063 | RB064 | RB065 | |
25.4% | 40.6% | 40.9% | 41.3% | 35.5% | ||
Bluebelle | RB071 | RB072 | RB073 | RB074 | RB075 | |
28.7% | 37.1% | 41.2% | 40.6% | 43.5% | ||
Gardenblue | RB081 | RB082 | RB083 | RB084 | RB085 | |
36.5% | 46.4% | 41.5% | 42.0% | 38.4% | ||
Woodard | RB091 | RB092 | RB093 | RB094 | RB095 | |
38.2% | 41.9% | 43.0% | 40.0% | 31.1% | ||
Red Pearl | RB101 | RB102 | RB103 | RB104 | RB105 | |
24.2% | 40.5% | 47.0% | 43.3% | 44.2% | ||
Homebell | RB111 | RB112 | RB113 | RB114 | RB115 | |
39.5% | 35.7% | 34.6% | 42.3% | 38.9% | ||
SB | O’Neal | SB011 | SB012 | SB013 | SB014 | SB015 |
25.6% | 34.4% | 32.6% | 32.3% | 26.5% | ||
Reveille | SB021 | SB022 | SB023 | SB024 | SB025 | |
34.2% | 33.1% | 36.0% | 39.0% | 31.2% | ||
Sunshineblue | SB031 | SB032 | SB033 | SB034 | SB035 | |
36.5% | 32.4% | 35.9% | 37.0% | 34.2% | ||
Sharpblue | SB041 | SB042 | SB043 | SB044 | SB045 | |
33.7% | 31.0% | 33.8% | 32.7% | 32.9% | ||
Flodablue | SB051 | SB052 | SB053 | SB054 | SB055 | |
31.1% | 29.4% | 32.0% | 32.7% | 32.0% | ||
NB | Spartan | NB011 | NB012 | NB013 | NB014 | NB015 |
32.7% | 27.9% | 21.4% | 34.9% | 24.3% | ||
Bluecrop | NB021 | NB022 | NB023 | NB024 | NB025 | |
19.0% | 25.2% | 29.9% | 32.5% | 22.1% | ||
Berkeley | NB031 | NB032 | NB033 | NB034 | NB035 | |
25.1% | 33.7% | 32.3% | 32.7% | 30.7% | ||
WB | Shashanbo | WB011 | WB012 | WB013 | WB014 | WB015 |
13.9% | 32.9% | 26.4% | 34.3% | 33.5% |
2.3. Cell Culture
2.4. Cell Proliferation Assay
2.5. Statistical Analysis
3. Results and Discussion
3.1. Seasonal Variation in Cell Inhibition of 80% Ethanol Extracts from Leaves of the Rabbit-Eye Blueberry Species (Vaccinium virgatum Aiton; RB Species) Collected in 2006
3.2. Comparison of Cultivars and Seasonal Variation in the Extracts of Blueberry Leaves Collected in 2008 on the Suppression of ATL Cell Growth
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Kai, H.; Fuse, T.; Kunitake, H.; Morishita, K.; Matsuno, K. Comparison of Cultivars and Seasonal Variation in Blueberry (Vaccinium Species) Leaf Extract on Adult T-Cell Leukemia Cell Line Growth Suppression. Medicines 2014, 1, 3-11. https://doi.org/10.3390/medicines1010003
Kai H, Fuse T, Kunitake H, Morishita K, Matsuno K. Comparison of Cultivars and Seasonal Variation in Blueberry (Vaccinium Species) Leaf Extract on Adult T-Cell Leukemia Cell Line Growth Suppression. Medicines. 2014; 1(1):3-11. https://doi.org/10.3390/medicines1010003
Chicago/Turabian StyleKai, Hisahiro, Takuichi Fuse, Hisato Kunitake, Kazuhiro Morishita, and Koji Matsuno. 2014. "Comparison of Cultivars and Seasonal Variation in Blueberry (Vaccinium Species) Leaf Extract on Adult T-Cell Leukemia Cell Line Growth Suppression" Medicines 1, no. 1: 3-11. https://doi.org/10.3390/medicines1010003