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Sci. Pharm. 2017, 85(2), 24; doi:10.3390/scipharm85020024

Assessment of Antimicrobial and Antioxidant Activities of Nepeta trachonitica: Analysis of Its Phenolic Compounds Using HPLC-MS/MS

1
Department of Chemistry, Faculty of Science, Erzincan University, Erzincan 24100, Turkey
2
Department of Biology, Faculty of Science, Bingol University, Bingol 12000, Turkey
3
Department of Biology, Faculty of Science, Muş Alparslan University, Muş 49250, Turkey
4
Department of Chemistry, Graduate School of Natural and Applied Sciences, Dicle University, Diyarbakır 21280, Turkey
5
Department of Chemistry, Faculty of Science, Atatürk University, Erzurum 25240, Turkey
6
Department of Nursing, School of Health, Muş Alparslan University, Mus 49250, Turkey
*
Author to whom correspondence should be addressed.
Academic Editor: Gernor Eller
Received: 5 February 2017 / Revised: 3 May 2017 / Accepted: 11 May 2017 / Published: 15 May 2017
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Abstract

Continuing our work on the sources of natural bioactive compounds, we evaluated the antimicrobial and antioxidant activities of Nepeta trachonitica as well as its major phenolic content using the high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS) technique. For antioxidant activity, ferric reducing antioxidant power (FRAP) and cupric ion reducing antioxidant capacity (CUPRAC) methods were performed to measure the reducing power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was employed to evaluate the radical scavenging activity of the sample. For antimicrobial activity, three Gram-positive and four Gram-negative microbial species as well as three fungi species were tested. N. trachonitica appeared to have reasonable antioxidant activity and decent antimicrobial activity as indicated by the inhibition of the organisms’ growth. The most susceptible species were Bacillus subtilis ATCC 6633 and Escherichia coli ATCC 11229 among the organisms tested. Ethanol extract of the plant has the highest effect on Saccharomyces cerevisiae but no effect on Yarrowia lipolytica. The HPLC-MS/MS analysis showed that at least 11 major phenolic compounds of N. trachonitica exist, the major ones being rosmarinic acid, chlorogenic acid and quinic acid. The obtained results suggest that N. trachonitica could be a promising source for food and nutraceutical industries because of its antimicrobial and antioxidant properties and phenolic compounds. View Full-Text
Keywords: Nepeta trachonitica; antimicrobial activity; antioxidant activity; phenolic compounds; HPLC-MS/MS Nepeta trachonitica; antimicrobial activity; antioxidant activity; phenolic compounds; HPLC-MS/MS
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MDPI and ACS Style

Köksal, E.; Tohma, H.; Kılıç, Ö.; Alan, Y.; Aras, A.; Gülçin, İ.; Bursal, E. Assessment of Antimicrobial and Antioxidant Activities of Nepeta trachonitica: Analysis of Its Phenolic Compounds Using HPLC-MS/MS. Sci. Pharm. 2017, 85, 24.

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