Next Article in Journal
A Validated Stability-Indicating HPLC Method for Simultaneous Determination of Amoxicillin and Enrofloxacin Combination in an Injectable Suspension
Previous Article in Journal
Natural Products as New Treatment Options for Trichomoniasis: A Molecular Docking Investigation
Article Menu

Export Article

Open AccessArticle
Sci. Pharm. 2017, 85(1), 3; doi:10.3390/scipharm85010003

Transmission Electron Microscopy of XDR Mycobacterium tuberculosis Isolates Grown on High Dose of Ofloxacin

1
Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak 3848176941, Iran
2
Department of Biology, Payame Noor University, Tehran 79134-13915, Iran
3
Research Institute for Epidemiology and Microbiology, Minsk 220114, Belarus
4
Institute for Pulmonology and Phthisiology, Minsk 220114, Belarus
*
Author to whom correspondence should be addressed.
Received: 23 September 2016 / Accepted: 3 January 2017 / Published: 2 February 2017
View Full-Text   |   Download PDF [764 KB, uploaded 2 February 2017]   |  

Abstract

The aim of the study was to investigate behavior of resistant Mycobacterium tuberculosis (MTB) isolates under a high dose of ofloxacin and its morphological changes. 19 extensively drug resistant (XDR) clinical isolates of MTB were grown on Löwenstein–Jensen medium containing progressively increasing concentrations of ofloxacin (2, 4, 8, 16, 32 mg/L). Ultra-structure analyses of resistant isolates grown on ofloxacin were conducted by transmission electron microscopy (TEM). Fixation was carried out by 4% glutaraldehyde in 0.1 M sodium cacodylate buffer on 300 mesh carbon formvar copper grid. The samples were negatively stained with uranium acetate suspension. All19XDRMTBisolatesweregrownandformedcoloniessuccessfullyon2,4,8mg/L,sevenisolates on16mg/L,andfourisolateson32mg/Lofloxacin. Morphologicalchangesandunusualformswere detected in 8, 16 and 32 mg/L ofloxacin at 43%, 76.5% and 81% of cells, respectively. Swollen form (protoplast like), ghost-like cell, degraded forms, and in a few cases, detached cytoplasm from cell wall were clearly detected in high drug concentrations in comparison to control. Changes in morphology were increased with increasing ofloxacin concentrations (p < 0.05). Some XDR isolates could be successfully grown on high doses of ofloxacin (32 mg/L), but with changes in morphology. It was concluded that several magnitudes of the drug doses could not prevent growth of drug resistant forms. View Full-Text
Keywords: Mycobacterium tuberculosis; transmission electron microscope; XDR; ofloxacin Mycobacterium tuberculosis; transmission electron microscope; XDR; ofloxacin
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Arjomandzadegan, M.; Sadrnia, M.; Titov, L.; Surkova, L.; Sarmadian, H.; Ghasemikhah, R.; Hosseiny, H. Transmission Electron Microscopy of XDR Mycobacterium tuberculosis Isolates Grown on High Dose of Ofloxacin. Sci. Pharm. 2017, 85, 3.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sci. Pharm. EISSN 2218-0532 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top